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Cryoprotection—Permeant vs nonpermeant additives
Cryobiology, 1974Abstract Chinese hamster ovary cells (CHO) were preserved at low temperatures with permeating and nonpermeating solutes. By making use of the temperature dependence of the diffusion of glycerol into cells it was possible to use this compared as either one or the other of these 2 classes of cryoprotectant.
R, Taylor +3 more
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Cryoprotectants for frozen red meats
Meat Science, 1998This present research explores the sensory characteristics of several cryoprotectant carbohydrates that could be added to frozen red meat. The cryoprotectants were sucrose, dsorbitol, maltodextrin DE 24-38 and synthetic polydextrose. The sweetness of aqueous solutions of sorbitol, maltodextrin and polydextrose was compared to standard 0.8% sucrose ...
A, Tomaniak, I, Tyszkiewicz, J, Komosa
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New cryoprotectant for cryorefractive surgery
Journal of Cataract and Refractive Surgery, 1991Cryorefractive surgeries, keratomileusis, keratophakia, and epikeratophakia cause destruction of keratocytes, which may result in postoperative corneal haze. We examined the effects of two cryoprotectants on keratocyte survival following freeze injury. We compared the ability of CPTES and the standard cryoprotectant KM-26 to prevent keratocyte death by
K, Kratz-Owens +2 more
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Cryoprotective additives and hypertonic hemolysis
Cryobiology, 1970Abstract The effects of three cryoprotective additives, dimethyl sulfoxide, sucrose and polyvinylpyrrolidone, on hemolysis of human red cells in hypertonic solutions of sodium chloride have been examined at room temperature. At the mole fractions of sodium chloride studied, polyvinylpyrrolidone reduced the level of hypertonic hemolysis; dimethyl ...
J, Farrant, A E, Woolgar
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Biochemistry of Cryoprotectants
1991The role of polyhydric alcohols in cryoprotection is probably the most extensively studied feature of insect cold hardiness. The importance of glycerol as a cryoprotectant was first recognized by R. W. Salt after he and others linked the presence of high levels of glycerol with winter hibernation, diapause, or freezing survival (Salt, 1957, 1959, 1961;
Kenneth B. Storey, Janet M. Storey
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Cryoprotectant toxicity in Caenorhabditis elegans
Cryobiology, 2019We have looked at the effects of the cryoprotectant M22 upon viability in the model organism C. elegans. M22 is a well-known vitrification solution which has been successfully used in the laboratory to preserve organs destined for transplantation. M22 reduces survival of C. elegans in a concentration-dependent manner. M22 at concentrations of 10% (v/v)
Patricia M, Tedesco +2 more
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Development and Application of Cryoprotectants
2018Cryopreservation involves the preservation of biological materials, including cells, embryos, tissues, and organs, at ultra-low temperatures (in a state of suspended animation), for a long period of time, and in a way that allows them to be restored whenever required.
Robin, Rajan, Kazuaki, Matsumura
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Cryoprotectants and cryoprotective effect in cryopreservation of gamete cells
2007Gamet hücrelerinin dondurulmasında kullanılan koruyucu maddeler (kryoprotektanlar) soğuk şoku ve donma esnasında gelişen diğer hasarlara karşı koruma sağlarlar. Kryoprotektanların hücrede toksik etkiye sahip olmaları, bu maddeler üzerindeki çalışmaları yoğunlaştırmış, ortama katılan eksternal kryoprotektanlarla, toksik etkimeler azaltılmaya ...
BUCAK, Mustafa Numan, TEKİN, Necmettin
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