Results 51 to 60 of about 57,341 (305)

Cryoprotectant-free cryopreservation of mammalian cells by superflash freezing

open access: yesProceedings of the National Academy of Sciences of the United States of America, 2019
Significance Cryopreservation is routinely used for the long-term storage of cells in various areas of academic, industrial, and clinical research.
Y. Akiyama   +4 more
semanticscholar   +1 more source

Effects of extender type, sperm volume, cryoprotectant concentration, cryopreservation and time duration on motility, survival and fertilisation rates of Mekong giant catfish sperm [PDF]

open access: yesMaejo International Journal of Science and Technology, 2010
The objectives of this study are to evaluate the effects of some basic factors, namely extender type, sperm volume, cryoprotectant concentration, cryopreservation and storage time, on the quality of Mekong giant catfish (MGC) sperm. The following results
Kriangsak Mengumphan
doaj  

Effect of Some Biopolymers on the Rheological Behavior of Surimi Gel

open access: yesMolecules, 2012
The objective of this study was to investigate the effect of selected biopolymers on the rheological properties of surimi. In our paper, we highlight the functional properties and rheological aspects of some starch mixtures used in surimi.
Takahiro Noda   +5 more
doaj   +1 more source

Vitrification Ability of Combined and Single Cryoprotective Agents

open access: yesPlants, 2021
Cryoprotective agents (CPA) are an important part of many current vitrification methods. The vitrification ability of CPAs influences the probability of the glass transition and water crystallization occurrence.
Milos Faltus   +2 more
doaj   +1 more source

Single cis‐elements in brassinosteroid‐induced upregulated genes are insufficient to recruit both redox states of the BIL1/BZR1 DNA‐binding domain

open access: yesFEBS Letters, EarlyView.
Phytohormone brassinosteroid‐induced gene regulation by the transcription factor BIL1/BZR1 involves redox‐dependent DNA‐binding alternation and interaction with the transcription factor PIF4. The reduced BIL1/BZR1 dimer binds preferred cis‐elements, while oxidation alters its oligomerization state and disrupts DNA‐binding ability.
Shohei Nosaki   +4 more
wiley   +1 more source

Role of Cryoprotectants in Preventing Quality Loss of Goat Oocytes after Warming by Examining Insulin-Like Growth Factor-1 and Bone Morphogenetic Protein-15 Expression

open access: yesJurnal Medik Veteriner
This research aimed to assess the efficacy of ethylene glycol-sucrose as an alternative cryoprotectant compared to commercial cryoprotectants by focusing on the expression of insulin-like growth factor-1 (IGF-1) and bone morphogenetic protein-15 (BMP-15)
Zahra Shabira   +6 more
doaj   +1 more source

Effects of the slow cooling during cryopreservation on the survival and morphology of Taiwan shoveljaw carp (Varicorhinus barbatulus) spermatozoa [PDF]

open access: yes, 2009
Over the past decades, pollution, overfishing, and habitat degradation have driven the population size of Taiwan shoveljaw carp down markedly in Taiwan.
Andrabi   +38 more
core   +2 more sources

Cryoprotectant Toxicity: Facts, Issues, and Questions

open access: yesRejuvenation Research, 2015
High levels of penetrating cryoprotectants (CPAs) can eliminate ice formation during cryopreservation of cells, tissues, and organs to cryogenic temperatures. But CPAs become increasingly toxic as concentration increases.
B. Best
semanticscholar   +1 more source

Growth Differentiation Factor 15 Elevation in the Central Nervous System Is Associated With Failure to Thrive in Alexander Disease

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Alexander disease (AxD) is a severe neurodegenerative disorder caused by gain‐of‐function mutations in the gene for GFAP, which lead to protein aggregation and a primary astrocytopathy. Symptoms vary, but failure to thrive (FTT) and frequent emesis are common and cause significant morbidity. Here we investigate GDF15, a member of the
Tracy L. Hagemann   +6 more
wiley   +1 more source

Lyophilization of Liposomal Formulations: Still Necessary, Still Challenging

open access: yesPharmaceutics, 2018
Nowadays, the freeze-drying of liposome dispersions is still necessary to provide a solid dosage form intended for different routes of administration (i.e., parenteral, oral, nasal and/or pulmonary).
Silvia Franzé   +4 more
doaj   +1 more source

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