Results 151 to 160 of about 3,438 (209)

The distribution of cryoprotective agents into lipid interfaces

Cryobiology, 1977
Abstract Capillary rise measurements indicate that the cryoprotective agents dimethyl sulfoxido, glycerol, sucrose, hydroxy ethyl starch, polyvinyl pyrrolidone, and dextran are surface active, suggesting the possibility that they distribute into the lipids of the cell membrane.
David Harris, Robert J. Williams
openaire   +3 more sources

Dimethylacetamide, a New Cryoprotective Agent for Platelets

Transfusion, 1971
Dimethylacetamide (DMAC) was used as cryoprotective agent for the preservation of platelets in the frozen state. The addition of dextrose to the platelet suspension greatly increased the cryoprotective effects of this agent. Rat platelets preserved with 5 per cent DMAC and 5 per cent dextrose showed a higher percentage of morphologically intact cells ...
J S Kim, J. Cavins, Isaac Djerassi, Albert J. Roy   +3 more
openaire   +3 more sources

Dielectric properties of supercooled cryoprotectant agents

Physics in Medicine and Biology, 1996
The dielectric properties of glycerol, propylene glycol, ethylene glycol and dimethyl sulphoxide have been measured in various aqueous concentrations generally sufficient to allow supercooling rather than freezing. The temperature range investigated is from -80 degrees C to +20 degrees C and the frequency range is 100 MHz to 2 GHz.
S Evans, S C Michelson
openaire   +3 more sources

Synthetic Polyampholytes as Macromolecular Cryoprotective Agents

Langmuir, 2018
A series of polyampholytes based on different molar ratios on N, N-dimethylaminopropyl methacrylamide (DMAPMA), acrylic acid (AA), and optionally, N- tert-butylacrylamide ( t-BuAAm), were prepared by free radical copolymerization, and tested as DMSO-free cryoprotective agents for 3T3 fibroblast cells by using a standard freeze-rethaw protocol ...
J. Zhao, M. A. Johnson, R. Fisher, N. A. D. Burke, H. D. H. Stöver   +4 more
openaire   +3 more sources

Cryoprotectant agent toxicity in porcine articular chondrocytes

Cryobiology, 2010
Large articular cartilage defects have proven difficult to treat and often result in osteoarthritis of the affected joint. Cryopreservation of articular cartilage can provide an increased supply of tissues for osteochondral allograft but cryoprotective agents are required; however, few studies have been performed on the toxicity of these agents.
Law, G. K., Elliott, J. A., Forbes, J. F., Weiss, A. D., McGann, L. E., Jomha, N. M.   +5 more
openaire   +4 more sources

Cryoprotective agent toxicity interactions in human articular chondrocytes [PDF]

Cryobiology, 2012
Background Vitrification is a method of cryopreservation by which cells and tissues can be preserved at low temperatures using cryoprotective agents (CPAs) at high concentrations (typically ⩾6.0 M) to limit the harmful effects of ice crystals that can form during cooling processes.
Law, G. K., Forbes, J. F., Elliott, J. A., McGann, L. E., Jomha, N. M., Prasad, V., Almansoori, K. A.   +6 more
openaire   +3 more sources

The role of cryoprotective agents as hydroxyl radical scavengers

Cryobiology, 1978
Abstract The classic cryoprotective agents dimethylsulfoxide and glycerol are hydroxyl radical scavengers. In addition the cryoprotective agents tetramethylurea, dimethylformide, dimethylurea and monomethylurea act as hydroxyl radical scavengers as shown by the inhibition of ethylene production from methional and the inhibition of methane production ...
James S. Miller, David G. Cornwell
openaire   +3 more sources

Osmometric Measurements of Cryoprotective Agent Permeation into Tissues

2020
Quantification of the amount of cryoprotective agent (CPA) in a tissue is an essential step in the design of successful cryopreservation protocols. This chapter details two inexpensive methods to measure cryoprotective agent permeation into tissues as functions of time.
Nadr M. Jomha, Leila Laouar, Janet A.W. Elliott, Nadia Shardt, Kezhou Wu, Kezhou Wu   +5 more
openaire   +3 more sources

Cryoprotectant agents for ovarian tissue vitrification: Systematic review

Cryobiology, 2021
Studies on the cryopreservation of ovarian tissue usually compare slow freezing versus vitrification and aim to optimize protocols, evaluate combinations or concentrations of cryoprotectant agents (CPAs), exposure time, and the addition of synthetic polymers.
Taynná El Cury-Silva, Monique E.G. Nunes, Maíra Casalechi, Fabio V. Comim, Jhenifer K. Rodrigues, Fernando M. Reis   +5 more
openaire   +3 more sources

Thermal Expansion Measurements of Cryoprotective Agents

Advances in Bioengineering, 2003
As part of an ongoing effort to characterize the mechanical behavior of biological tissues in the cryogenic temperature range, the current study focuses on the thermal expansion measurements of cryoprotective agents. This study focuses particularly on the upper part of the cryogenic temperature range, where the cryoprotectant behaves like low viscous ...
Yoed Rabin, Ernest Bell
openaire   +2 more sources