Results 161 to 170 of about 3,438 (209)

Perfusion of rabbit kidneys with cryoprotective agents

Cryobiology, 1972
Abstract Rabbit kidneys were perfused at 5 or 37 °C for 2 hr with 2 m solutions of ethylene glycol, glycerol, or dimethylsulfoxide. It was found that each cryoprotectant caused an initial decrease in vascular resistance which was greater at 5 than at 37 °C but that dimethylsulfoxide caused a subsequent increase in resistance which was due to ...
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A Dilution-Filtration System for Removing Cryoprotective Agents

Journal of Biomechanical Engineering, 2011
In most cryopreservation applications, the final concentrations of cryoprotective agents (CPAs) must be reduced to biocompatible levels. However, traditional methods for removing CPAs usually have disadvantages of operation complexity, time consumption, and ease of contamination, especially for the applications involving large volumes of cell ...
Weiping Ding, Zhiquan Shu, Shelly Heimfeld, Xiaoming Zhou, Jae Hyun Chung, Zhong Liu, Carolyn Liu, Pingan Du, Dayong Gao   +8 more
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Recent insights on the role of cryoprotective agents in vitrification

Cryobiology, 1989
In recent efforts to produce cryoprotective solutions which cause either complete, or almost complete, vitrification of the cell or tissue material, increasingly complex cocktails of solutes have invariably been used. Why some of these solutes are so much more effective in suppressing ice formation than other, related solutes has never been clear.
Maria Forsyth, Douglas R. MacFarlane
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Deep eutectic solvents as cryoprotective agents for mammalian cells

Journal of Materials Chemistry B, 2022
A deep eutectic solvent is an effective cryoprotective agent for several human cell lines.
Saffron J. Bryant, Miyah N. Awad, Aaron Elbourne, Andrew J. Christofferson, Andrew V. Martin, Nastaran Meftahi, Calum J. Drummond, Tamar L. Greaves, Gary Bryant   +8 more
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Effect of cryoprotective agents on rat cutaneous nerves

Cryobiology, 1975
Abstract Desheathed rat cutaneous nerves were exposed to various concentrations of ethylene glycol (EG), glycerol and dimethyl sulfoxide (DMSO) at temperatures of 1, 24, and 38 °C for periods of time ranging from 5 to 60 min. Measurements of the percent recovery of the original action potential (AP) were determined after removal of the cryoprotective
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Permeation of several cryoprotectant agents into porcine articular cartilage

Cryobiology, 2009
Objective: Osteochondral allografting is an effective method to treat large osteochondral defects but difficulties in tissue preservation have significantly limited the application of this technique. Successful cryopreservation of articular cartilage (AC) could improve the clinical availability of osteochondral tissue and enhance clinical outcomes but ...
Rekieh, K., Abazari, A., Elliott, J.A., Jomha, N.M., Law, G.K., McGann, L.E.   +5 more
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Platelet Preservation by Freezing. Use of Dimethylsulfoxide as Cryoprotective Agent

Transfusion, 1974
Variables important in the preservation of platelets by freezing with dimethylsulfoxide (DMSO) as cryoprotective agent were studied in normal volunteers and thrombocytopenic patients. Use of 5 per cent DMSO and a freezing rate of 1–3 C/min‐ute yielded optimal preservation of platelet viability.
N. L. Abdou, Frank H. Gardner, Seyed N. Sayar, Scott Murphy   +3 more
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The mechanism of action of low temperature and cryoprotective agents on immunoproteins

Cryobiology, 1978
Abstract The freezing and thawing of specific antitoxic serum proteins without a cryoprotective agent results in a reduction of titer. A freezing regimen has been chosen and the cryoprotectant PEO-400 has been added to prevent a reduction in activity of antitetanus serum after 6 months of storage in liquid nitrogen. The study of dielectric properties
A.A. Tsutsayeva, I.I. Gavrilova, V.M. Markova, L.V. Ivanov, G.G. Medvedyeva, Y.A. Itkin, V.A. Moiseyev, A.L. Markovsky, N.S. Pushkar   +8 more
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Identification of new cryoprotective agents for cultured mammalian cells

In Vitro, 1983
Thirty-one compounds have been identified that act as cryoprotective agents for cultured mammalian cells. Eight compounds were comparable to dimethylsulfoxide (DMSO) in cryoprotective effectiveness. Many of the cryoprotective compounds studied also (a) promote cell fusion and (b) induce cell differentiation in erythroleukemia and other cell systems ...
Robert J. Klebe, Melodee G. Mancuso
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Evaluation of ethylene glycol as a cryoprotective agent for blood platelets

Cryobiology, 1973
Abstract (1) Ethylene glycol appears to penetrate blood platelets rapidly and is nontoxic in high concentrations. (2) Higher concentrations are tolerated when potassium is present in the suspending medium. (3) Although ethylene glycol satisfied the basic theoretical criteria for a penetrating cryoprotectant, it failed to result in sufficiently high ...
Richard A. Kahn, Linda J. Flinton
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