Functionality of Chimeric E2 Glycoproteins of BVDV and CSFV in Virus Replication
An intriguing difference between the E2 glycoprotein of CSFV and the other groups of pestiviruses (nonCSFV) is a lack of two cysteine residues on positions cysteine 751 and 798. Other groups of pestivirus are not restricted to one species as swine, whereas CSFV is restricted to swine and wild boar. We constructed chimeric CSFV/BVDV E2 genes based on a
van Gennip, H.G.P. +3 more
openaire +2 more sources
Infectivity of PCV2 and CSFV in different porcine cell lines.
3D4/31, ST and PK15 cell lines were inoculated with PCV2 at MOI = 1, 4, 7 and 10 or with CSFV at MOI = 0.03 and 0.1, respectively. After culture for 72 h, cells were fixed and stained for IFA, and then four fields were randomly chosen to count the ...
Boli Hu (750407) +9 more
core +1 more source
Porcine circovirus 2 (PCV2) remains a major swine pathogen, and interpretation of capsid protein (Cap)–based serology is challenging in vaccinated populations because vaccine‐induced Cap antibodies overlap with replication‐associated serological signals.
Mingxi Gou +8 more
wiley +1 more source
Subcellular localization of viral proteins in PK15 cells coinfected with PCV2 and CSFV.
PK15-CSFV cells were seeded on glass bottom dishes and inoculated with PCV2 at MOI = 10. At indicated time points after PCV2 infection, cells were fixed and immunostained for PCV2 Cap (green) and CSFV E2 protein (red) as well as DAPI-stained for the ...
Boli Hu (750407) +9 more
core +1 more source
Emerging Challenges and Advances in Porcine Circovirus: A Decade in Review
Over the past decade, porcine circoviruses (PCVs) have continued to pose a significant threat to global swine health, and pivotal discoveries have significantly reshaped our understanding of their biology and control. Extensive genomic surveillance has expanded porcine circovirus 2 (PCV2) genotyping from four to at least eight lineages, with PCV2d now ...
Jiawei Zheng +4 more
wiley +1 more source
Isolation and Pathogenicity of a Getah Virus With a Novel Deletion in the 3′ UTR
The spread of Getah virus (GETV) in China has become increasingly severe due to long‐term neglect and the lack of vaccines, posing a threat to animal safety and public health. In this study, we isolated and identified a novel GETV strain, designated GETV‐CX7.
Yawei Sun +11 more
wiley +1 more source
Enzyme-linked immunosorbent assay using a virus type-specific peptide based on a subdomain of envelope protein e(rns) for serologic diagnosis of pestivirus infections in swine [PDF]
Peptides deduced from the C-terminal end (residues 191 to 227) of pestivirus envelope protein Erns were used to develop enzyme-linked immunosorbent assays (ELISAs) to measure specifically antibodies against different types of pestiviruses.
Langedijk, J.P. +5 more
core +1 more source
METTL14 promotes CSFV replication.
(A and C) PK-15, IPEC-J2, and ST cells were transfected with siMETTL14 or siCtrl (A) or pEGFP-METTL14 (0.2, 0.5, and 1 μg) (C) and infected with CSFV (MOI = 1). At 24 hpi, cells were extracted RNA or lysated for RT-qPCR or virus titration.
Bing-qian Zhao (18275791) +8 more
core +1 more source
Crystallographic statistics for CSFV NS4A37NS3 crystal.
Crystallographic statistics for CSFV NS4A37NS3 crystal.
Félix A. Rey (3254583) +3 more
core +1 more source
Effects of astragalus polysaccharide on the adhesion-related immune response of endothelial cells stimulated with CSFVin vitro [PDF]
BackgroundAstragalus polysaccharide (APS) has immunomodulatory activities on porcine peripheral blood mononuclear cells. The immunomodulatory effects of APS on porcine endothelial cells (ECs) expose to classical swine fever virus (CSFV) remain unknown ...
Liuan Li +3 more
core +3 more sources

