The stringent response in Myxococcus xanthus is regulated by SocE and the CsgA C-signaling protein [PDF]
Myxococcus xanthus fruiting body development is induced by amino acid limitation. The decision to grow or develop is established by the RelA-dependent stringent response and A-signaling. We identified two new members of this regulatory hierarchy, socE and the C-signaling gene csgA.
Eugene W. Crawford, Lawrence J. Shimkets
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To counteract the negative effect of salt overconsumption on health, strategies have been developed to reduce the salt content in food products. Among them, two promising strategies based on odour-induced saltiness enhancement and the heterogeneous ...
Marion Emorine +6 more
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Recombinant Proteins: A Molecular Tool to Understand Marine Adhesion and to Advance Biomaterials. [PDF]
The production of recombinant proteins represents a fundamental step in the characterisation of marine invertebrate adhesives and in the development of bio‐inspired glues. The association of these proteins with other components such as ions, proteins, polysaccharides, or polymers enables the fabrication of biomaterials for various healthcare ...
Whaite A +4 more
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Single molecule studies of the bacterial curli protein CsgA reveal a structurally dynamic monomeric structure [PDF]
Abstract Functional amyloid forms the scaffold that supports bacterial communities, making them an important antimicrobial target. In particular, the subunit of curli fibrils, CsgA, has recently had its fibril assembly deciphered by cryoEM. However, the folding of CsgA has yet to be careful examined.
Daniel A. N. Foster, Derek R. Dee
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CsgA, an extracellular protein essential for Myxococcus xanthus development [PDF]
CsgA mutants of Myxococcus xanthus appear to be defective in producing an extracellular molecule essential for the developmental behaviors of this bacterium. The csgA gene encodes a 17.7-kilodalton polypeptide whose function and cellular location were investigated with immunological probes. Large quantities of the CsgA gene product were obtained from a
L J, Shimkets, H, Rafiee
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CsgA Production by Escherichia coli O157:H7 Alters Attachment to Abiotic Surfaces in Some Growth Environments [PDF]
ABSTRACT The role of curli expression in attachment of Escherichia coli O157:H7 to glass, Teflon, and stainless steel (SS) was investigated through the creation of csgA knockout mutants in two isolates of E. coli O157:H7.
Rebecca M. Goulter-Thorsen +4 more
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The curli nucleator protein, CsgB, contains an amyloidogenic domain that directs CsgA polymerization [PDF]
Curli are functional amyloid fibers assembled by enteric bacteria such as Escherichia coli and Salmonella spp. In E. coli , the polymerization of the major curli fiber subunit protein CsgA into an amyloid fiber depends on the minor curli subunit protein, CsgB.
Neal D. Hammer +2 more
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Tuning Functional Amyloid Formation Through Disulfide Engineering
Many organisms produce “functional” amyloid fibers, which are stable protein polymers that serve many roles in cellular biology. Certain Enterobacteriaceae assemble functional amyloid fibers called curli that are the main protein component of the biofilm
Anthony Balistreri +3 more
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Myxococcus CsgA, Drosophila Sniffer, and human HSD10 are cardiolipin phospholipases [PDF]
Myxococcus xanthus development requires CsgA, a member of the short-chain alcohol dehydrogenase (SCAD) family of proteins. We show that CsgA and SocA, a protein that can replace CsgA function in vivo, oxidize the 2′-OH glycerol moiety on cardiolipin and phosphatidylglycerol to produce diacylglycerol (DAG), dihydroxyacetone, and orthophosphate.
Boynton, Tye O'Hara +1 more
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Influence of Prebiotic Fructans on Retronasal Aroma from Elderly Individuals
This study investigates for the first time the role of fructans with prebiotic effects (oligofructose and inulin) on retronasal aroma among elderly individuals.
Carolina Muñoz-González +4 more
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