Effect of αs1-casein (CSN1S1) genotype on milk CSN1S1 content in Malagueña and Murciano-Granadina goats [PDF]
There is substantial evidence showing that the polymorphism of the goat αs1-casein (CSN1S1) gene has a major effect on milk protein, casein and fat content as well as on cheese yield. However, its influence on the synthesis rate of CSN1S1 has been less studied, with measurements only available in French breeds.
Caravaca, F +11 more
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Comparative analysis of gene sequence of goat CSN1S1 F and N alleles and characterization of CSN1S1 transcript variants in mammary gland [PDF]
In this paper, we report the full characterization, at DNA and RNA level, of the defective goat CSN1S1 F and N alleles and an extensive comparison with the A allele counterpart. By utilizing genomic DNA as template, we amplified the whole CSN1S1 gene plus 1972/3 nucleotides of the 5' region plus 610 nucleotides of the 3' region of the goat CSN1S1 N and
RAMUNNO, LUIGI +7 more
core +6 more sources
Genomics and proteomics of deleted ovine CSN1S1∗I [PDF]
Abstract Ovine α s1 -casein ( CSN1S1 ) allele I ( CSN1S1 ∗ I ) was characterized at the molecular genetic and protein level. Sequencing of CSN1S1 cDNA and mature protein showed the absence of exon 7 from CSN1S1 ∗ I in comparison with the C ″ genetic variant of the C phenotype. This allelic aberration is correlated with a sequence difference in
GIAMBRA I. J. +3 more
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Genetic polymorphism at the CSN1S1 gene in Girgentana dairy goat breed [PDF]
The aim of this work was to evaluate the variability of the as1-casein locus in the endangered Girgentana dairy goat breed in order to define genetic improvement and a conservation program for this breed. The study was performed on 200 dairy goats by means of different PCR protocols. The most frequent alleles were A (0.590) and F (0.290) followed by B (
S. Mastrangelo +3 more
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Association of the CSN1S1 gene polymorphism with milk composition traits in Jamunapari goat [PDF]
The genetic polymorphism of the CSN1S1 (casein alpha-s1) locus was investigated in Jamunapari goat breed by Sodium Dodicyle Sulphate – Poly Acrylamide Gel Electrophoresis (SDS-PAGE) and Polymerase Chain Reaction – Restriction Fragment Length Polymorphism (PCR-RFLP). A total of 122 milk and blood samples were collected. The SDS-PAGE pattern revealed the
Pallavi Singh +3 more
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The autochthonous agerolese breed: genetic structure at CSN1S1 and CSN3 loci. [PDF]
This work arises as targets the genetic characterization at CSN1S1 and CSN3 loci, using techniques of molecular biology, of the agerolese cattle, an autochthonous genetic type reared in Campania, which is at risk of extinction.In particular, we proceeded to the identification of the CSN1S1 G and CSN3 B allele carriers, whose choice was dictated by the ...
APICELLA, ELISA +5 more
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A reliable method for characterization of the goat CSN1S1 E allele
Livestock Science, 2009Abstract Goat αs 1 -casein, coded by the CSN1S1 gene, is a highly polymorphic protein. The E allele is characterized by the insertion of a long interspersed nuclear element (LINE) in the 19th exon of the gene. This insertion is probably responsible for the reduced levels of αs 1 -casein found in milk from animals expressing the E allele.
DETTORI, Maria Luisa +5 more
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Short communication: CSN1S1-CSN3 (αS1-κ-casein) composite genotypes affect detailed milk protein composition of Mediterranean water buffalo [PDF]
The aim of the study was to investigate the effect of composite CSN1S1-CSN3 [α(S1)-κ-casein (CN)] genotype on milk protein composition in Mediterranean water buffalo.
Paolo Carnier +2 more
exaly +2 more sources
Individuazione e caratterizzazione dei retroposoni nel gene CSN1S1 di capra. [PDF]
Il genoma bovino contiene tre tipi di elementi di origine retroposonica: Bov-B (1,8%), Bov-A2 (0,5%) e Bov-tA (1,6%). Gli elementi Bov-B sono LINEs il cui 5’ è caratteristicamente rappresentato da un elemento PstI. Probabilmente, da questi si è originato il Bov-A, componente sia del Bov-tA (etero-dimero di tRNAGly, pseudogene per un tRNA e un Bov-A ...
COSENZA, GIANFRANCO +6 more
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Molecular characterization of the goat CSN1S101 allele
Journal of Dairy Research, 2003Caseins (αs1, β, αs2, e κ) represent about 80% of the whole protein content of ruminant milk. Each of these proteins is encoded by single copy genes (CSN1S1, CSN2, CSN1S2 and CSN3, respectively) clustered on a ∼200-kb segment of chromosome 6 (Ferretti et al. 1990; Gallagher et al. 1994) in the order: CSN1S1, CSN2, CSN1S2 and CSN3 (Mercier & Vilotte,
COSENZA, GIANFRANCO +5 more
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