Results 71 to 80 of about 2,133,258 (309)

High-throughput three-dimensional spheroid tumor model using a novel stamp-like tool

Journal of Tissue Engineering, 2019
Spheroid culture is a widely used three-dimensional culture technology that simulates the three-dimensional structure of tumors in vivo and has been considered a good model for tumor research.
Wei Liao, Jieqing Wang, Jiecheng Xu, Fuyu You, Mingxin Pan, Xiaoping Xu, Jun Weng, Xu Han, Shao Li, Yang Li, Kangyan Liang, Qing Peng, Yi Gao   +12 more
doaj   +1 more source

A simple, versatile and sensitive cell-based assay for prions from various species. [PDF]

PLoS ONE, 2011
Detection and quantification of prion infectivity is a crucial step for various fundamental and applied aspects of prion research. Identification of cell lines highly sensitive to prion infection led to the development of cell-based titration procedures ...
Zaira E Arellano-Anaya, Jimmy Savistchenko, Jacinthe Mathey, Alvina Huor, Caroline Lacroux, Olivier Andréoletti, Didier Vilette   +6 more
doaj   +1 more source

Growth and photosynthesis responses of microcystin (MC)- and non-MC-producing Microcystis strains during co-culture with the submerged macrophyte Myriophyllum spicatum

Water Science and Technology, 2022
The growth and photosynthetic responses of microcystin (MC)- and non-MC-producing Microcystis to the submerged macrophyte Myriophyllum spicatum were investigated under plant-Microcystis co-culture conditions (PMC + , PMC−), in comparison with their ...
Yunni Gao, Hui Yang, Jing Dong, Man Zhang, Xiaofei Gao, Jingxiao Zhang, Mei Li, Xuejun Li   +7 more
doaj   +1 more source

History and sensitivity comparison of two standard whole-sediment toxicity tests with crustaceans : the amphipod Hyalella azteca and the ostracod Heterocypris incongruens microbiotest [PDF]

, 2015
The review first details the development of the test procedures with Hyalella azteca which historically emerged as one of the recommended test species for whole-sediment assays and its gradual standardization and endorsement by national and international
Blaise, C, De Cooman, W, Detemmerman, L, Elst, R, Janssen, Colin, Persoone, Guido   +5 more
core   +2 more sources

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Comparison of Integrated Cell Culture -RT-PCR & Cell Culture Methods for Detection of Enteroviruses

Iranian Journal of Public Health, 2009
Background: Generally, sewage exposed water could be potentially contaminated with enteroviruses. For this reason, en­terovirus isolation from sewage specimens is one of the most sensitive indicators for virus circulation in the population. We evalu­ated
M Kargar , S Sadeghipour , B Zarei Mahmoudabadi , R Nategh   +3 more
doaj  

Inhibitors of the interferon response enhance virus replication in vitro.

PLoS ONE, 2014
Virus replication efficiency is influenced by two conflicting factors, kinetics of the cellular interferon (IFN) response and induction of an antiviral state versus speed of virus replication and virus-induced inhibition of the IFN response.
Claire E Stewart, Richard E Randall, Catherine S Adamson   +2 more
doaj   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Ethical implications of culturally (in)sensitive practice in psychotherapy [PDF]

Psihološka Istraživanja, 2016
Until the 80s of the 20th century psychotherapy was based on the assumption that the universality of human experience was of greater importance for the psychotherapy process than specific cultural heritage of the client and the therapist, and therefore ...
Petrović Nikola M.
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source