Results 1 to 10 of about 311,317 (138)

Effect of Concentrated Fibroblast-Conditioned Media on In Vitro Maintenance of Rat Primary Hepatocyte. [PDF]

PLoS ONE, 2016
The effects of concentrated fibroblast-conditioned media were tested to determine whether hepatocyte function can be maintained without direct contact between hepatocytes and fibroblasts.
Dayeong Jeong, Chungmin Han, Inhye Kang, Hyun Taek Park, Jiyoon Kim, Hayoung Ryu, Yong Song Gho, Jaesung Park   +7 more
doaj   +4 more sources

Optimized exosome isolation protocol for cell culture supernatant and human plasma [PDF]

Journal of Extracellular Vesicles, 2015
Extracellular vesicles represent a rich source of novel biomarkers in the diagnosis and prognosis of disease. However, there is currently limited information elucidating the most efficient methods for obtaining high yields of pure exosomes, a subset of ...
Richard J. Lobb, Melanie Becker, Shu Wen Wen, Christina S. F. Wong, Adrian P. Wiegmans, Antoine Leimgruber, Andreas Möller   +6 more
doaj   +6 more sources

Fibrotic effect of monoculture, conditioned media and coculture of fibroblast NIH3T3 cells on collagen secretion and mRNA TGF-β expressions [PDF]

Pharmaciana, 2023
Fibrosis is common profound in diabetes condition, they characterized by accumulation of extracellular matrix in tissue then cause tissue injury. The study aim to investigate effect of monoculture, conditioned media, and coculture fibroblast of NIH3T3 ...
Rizki Awaluddin, Dwi Aris Agung Nugrahaningsih, Eti Nurwening Solikhah, Prasetyastuti   +3 more
doaj   +1 more source

The impact of the limbal niche interactions on the self-renewal capability of limbal epithelial stem cells [PDF]

Frontiers in Cell and Developmental Biology
IntroductionThe corneal homeostasis is maintained by limbal epithelial stem cells (LESCs), which reside in the limbal niche. This microenvironment comprises the cells, the extracellular matrix (ECM), and their interactions that balance the quiescent and ...
Sara Aghazadeh, Qiuyue Peng, Fereshteh Dardmeh, Jesper Østergaard Hjortdal, Vladimir Zachar, Hiva Alipour   +5 more
doaj   +2 more sources

Selecting Normalizers for MicroRNA RT-qPCR Expression Analysis in Murine Preimplantation Embryos and the Associated Conditioned Culture Media

Journal of Developmental Biology, 2023
Normalizing RT-qPCR miRNA datasets that encompass numerous preimplantation embryo stages requires the identification of miRNAs that may be used as stable reference genes.
David C. Hawke, Andrew J. Watson, Dean H. Betts   +2 more
doaj   +1 more source

Human esophageal myofibroblasts increase squamous epithelial thickness via paracrine mechanisms in an in vitro model of gastroesophageal reflux disease

PLoS ONE, 2020
The pathogenesis of esophageal injury in gastroesophageal reflux disease (GERD) is incompletely understood. We modeled exposure of human esophageal myofibroblasts (HEMFs) to gastroesophageal reflux by repeated treatment with pH 4.5 and pH 4.5 bile salts ...
Liping Hu, Chunying Zhang, Kevin Yang, Meng Li, Anisa Shaker, Nicholas Clemons   +5 more
doaj   +3 more sources

Characterisation of human embryonic stem cells conditioning media by 1H-nuclear magnetic resonance spectroscopy. [PDF]

PLoS ONE, 2011
BACKGROUND: Cell culture media conditioned by human foreskin fibroblasts (HFFs) provide a complex supplement of protein and metabolic factors that support in vitro proliferation of human embryonic stem cells (hESCs).
David A MacIntyre, Darío Melguizo Sanchís, Beatriz Jiménez, Rubén Moreno, Miodrag Stojkovic, Antonio Pineda-Lucena   +5 more
doaj   +1 more source

Growth Factors Released from Advanced Platelet-Rich Fibrin in the Presence of Calcium-Based Silicate Materials and Their Impact on the Viability and Migration of Stem Cells of Apical Papilla

Dentistry Journal, 2023
Advanced platelet-rich fibrin (A-PRF) provides the scaffold and growth factors necessary for stem cells to proliferate and differentiate in successful regenerative endodontic procedures.
Cristine Smoczer, Kenneth R. Yuth, Mazin A. Askar, Laura A. Young, Susan B. Paurazas   +4 more
doaj   +1 more source

Expression, Tissue Distribution and Function of miR-21 in Esophageal Squamous Cell Carcinoma [PDF]

, 2013
Objective:MiR-21 is an oncomir expressed by malignant cells and/or tumor microenvironment components. In this study we focused on understanding the effects of stromal miR-21 on esophageal malignant cells.Design:MiR-21 expression was evaluated in formalin-
Calin, G.A., Mowla, S.J., Naghshvar, F., Nouraee, N., Omidi, A.A., Samaei, N.M., Semnani, S., Van Roosbroeck, K., Vasei, M.   +8 more
core   +10 more sources

Laser-Modified Ti Surface Improves Paracrine Osteogenesis by Modulating the Expression of DKK1 in Osteoblasts

Journal of Functional Biomaterials, 2023
Titanium surface modifications are widely used to modulate cellular behavior by recognition of topographical cues. However, how those modifications affect the expression of mediators that will influence neighboring cells is still elusive.
Jorge Felipe Lima Teixeira, João Antônio Chaves de Souza, Fernando Augusto Cintra Magalhães, Guilherme José Pimentel Lopes de Oliveira, José Bernardo de Santis, Carlos Alberto de Souza Costa, Pedro Paulo Chaves de Souza   +6 more
doaj   +1 more source