Results 121 to 130 of about 437,976 (307)

The influence of serum-free culture conditions on skeletal muscle differentiation in a tissue-engineered model

, 2008
The influence of differentiation medium (DM) components on C2C12 murine myoblast differentiation has only been studied in monolayer cultures. Serum-free formulations have been applied that omit the use of sera with unknown composition.
Baaijens, FPT Frank, Boonen, KJM Kristel, Oomens, CWJ Cees, Bouten, C.V.C., Bouten, C.V.C.; id_orcid, Gawlitta, D Debby, Bouten, CVC Carlijn, Boonen, K.J.M., Oomens, C.W.J., Baaijens, F.P.T., Gawlitta, D.   +10 more
core   +1 more source

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

FEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim, Brittany Taylor, Shannon Bolten, Christian L. Eberly, Mahliya Abdurahman, T. Michael Creed, Acong Yang, Taylor L. Hatchet, Tristan Dyson, Shuo Gu, Curt I. Civin, Tami J. Kingsbury   +11 more
wiley   +1 more source

Developing Serum-Free Media Via Bioprocessing For Cultivated Seafood Products

, 2022
Global food production management has become a challenge with an anticipated population of 10 billion people by 2050 and the ongoing COVID-19 epidemic. Seafood is a vital food source due to its widespread consumption, excellent nutrient profile, and low ...
Batish, Inayat
core  

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

FEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Attachment promoting compounds significantly enhance cell proliferation and purity of bovine satellite cells grown on microcarriers in the absence of serum

Frontiers in Bioengineering and Biotechnology
IntroductionTo bring cultivated beef to the market, a scalable system that can support growth of bovine satellite cells (bSCs) in a serum-free and preferably also animal-free medium is of utmost importance.
Vincent Bodiou, Vincent Bodiou, Anitha Ajith Kumar, Edoardo Massarelli, Tessa van Haaften, Mark J. Post, Mark J. Post, Panagiota Moutsatsou   +7 more
doaj   +1 more source

The reflection of language in culture and identity: A case of Basotho in the Free State Province and Lesotho. [PDF]

, 2018
Published ThesisThe research investigates the relationship between language, culture and identity among the Basotho residing in the Free State Province of South Africa and Lesotho. The study attempts to reveal how each of the elements namely; language,
Mokuoane, Matsie Cassandra Ntsana
core  

Increased myelination efficiency by transplanted OPCs in older slices and serum-free media.

, 2013
Representative images comparing transplantation of CMFDA-labelled OPCs into 3 DIV organotypic slices cultured in serum-containing media (A–C), 16 DIV organotypic cerebellar slices cultured in serum-containing media (D–F), and 16 DIV organotypic ...
Sarah-Jane Bull (315171), Jenea M. Bin (315169), Jack P. Antel (315172), Timothy E. Kennedy (205904), Soo Yuen Leong (315170)   +4 more
core   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source