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Exploring cost reduction strategies for serum free media development [PDF]

open access: yesnpj Science of Food
Cultivated meat production offers solutions in addressing global food security and sustainability challenges. However, serum-free media (SFM) used in cultivating the cells are expensive, contributing to at least 50% of variable operating costs.
Jun Ping Quek   +7 more
doaj   +4 more sources

Comparative analysis of mesenchymal stem cells cultivated in serum free media [PDF]

open access: yesScientific Reports, 2022
Stem cells are attractive candidates for the regeneration of tissue and organ. Mesenchymal stem cells (MSCs) have been extensively investigated for their potential applications in regenerative medicine and cell therapy. For developing effective stem cell
Joo Youn Lee   +14 more
doaj   +5 more sources

Use of serum-free media for peripheral blood mononuclear cell culture and the impact on T and B cell readouts [PDF]

open access: yesFrontiers in Toxicology
IntroductionAs part of a wider programme of work developing next-generation risk assessment approaches (NGRA) using non-animal methods (NAMs) for safety assessment of materials, Unilever SEAC is exploring the use of a peripheral blood mononuclear cell ...
Stella Cochrane   +2 more
doaj   +4 more sources

Cultivation, cryopreservation and resuscitation of Theileria annulata transformed cells in serum-free media [PDF]

open access: yesFrontiers in Veterinary Science, 2022
IntroductionTropical theileriosis is a protozoan disease caused by Theileria annulata that affects cattle in Northern Africa, the Middle East and Asia where vector ticks of the genus Hyalomma occur.
Khawla Elati   +8 more
doaj   +2 more sources

The Comparison of Commercial Serum-Free Media for Hanwoo Satellite Cell Proliferation and the Role of Fibroblast Growth Factor 2 [PDF]

open access: yesFood Science of Animal Resources, 2023
Fetal bovine serum (FBS), which contains various nutrients, comprises 20% of the growth medium for cell-cultivated meat. However, ethical, cost, and scientific issues, necesitates identification of alternatives.
In-sun Yu   +3 more
doaj   +2 more sources

The effect of serum-free media on the metabolic yields and growth rates of C2C12 cells in the context of cultivated meat production

open access: yesFuture Foods, 2023
Successful commercialization of cultivated meat products as alternative sources of dietary protein for a growing global population will require development of efficient and inexpensive serum-free cell culture media.
Edward N. O'Neill   +6 more
doaj   +3 more sources

The Characterization of Serum-Free Media on Human Mesenchymal Stem Cell Fibrochondrogenesis [PDF]

open access: yesBioengineering
Developing fibrochondrogenic serum-free media is important for regenerating diseased and injured fibrocartilage but no defined protocols exist. Towards this goal, we characterized the effect of four candidate fibrochondrogenic serum-free media containing
Ka Yu Carissa Kwan   +7 more
doaj   +2 more sources

Serum-free media for the growth of primary bovine myoblasts. [PDF]

open access: yesCytotechnology, 2020
AbstractThe demand for meat is expected to exceed production capacity by livestock in the coming decennia. Therefore, cultured beef might be a viable alternative to traditional livestock-derived beef. One of the problems however is the sustainability of cultured beef through the use of fetal bovine serum.
Kolkmann AM   +4 more
europepmc   +5 more sources

Development of peptone-based serum-free media to support Vero CCL-81 cell proliferation and optimize SARS-CoV2 viral production [PDF]

open access: yesHeliyon
Background: Developing an optimal media for Vero cell lines is crucial as it directly influences cell survival, proliferation, and virus production. The use of serum in cell culture raises safety concerns in biological production.
Suryo Kuncorojakti   +10 more
doaj   +2 more sources

The effect of macromolecular crowders as a supplement to serum free media on human corneal stromal cells proliferation and marker expression [PDF]

open access: yesScientific Reports
Native human corneal stromal keratocytes (HCSKs) deposit extracellular matrix (ECM) to maintain corneal homeostasis, repair, and regeneration. Adding macromolecular crowders (MMCs) to culture media encourages HCSKs to produce ECM in vitro.
Weaam Ahmed Sultan, Che J. Connon
doaj   +2 more sources

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