Results 41 to 50 of about 730,822 (283)

Media Activism as Movement? Collective Identity Formation in the World Forum of Free Media

, 2017
More than simply tools used by social movements to reach other substantive aims, media are increasingly becoming subjects of activism. This article contributes to advancing understanding of such media-focused activism through a case study of the World ...
Hilde C. Stephansen, Stephansen, Hilde C., Stephansen, H.C.   +2 more
core   +2 more sources

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

A low percentage of autologous serum can replace bovine serum to engineer human nasal cartilage [PDF]

, 2008
For the generation of cell-based therapeutic products, it would be preferable to avoid the use of animal-derived components. Our study thus aimed at investigating the possibility to replace foetal bovine serum (FBS) with autologous serum (AS) for the ...
Farhadi, J., J Farhadi, Barbero, A., F Wolf, M Haug, Haug, M., C Candrian, Wolf, F., Martin, I., Candrian, C., I Martin, A Barbero   +11 more
core  

Serum-free medium containing bFGF and PDGF facilitates isolation and expansion of canine Ad-MSCs.

, 2019
A. Schematic to evaluate serum-free medium containing bFGF and PDGF for direct derivation and expansion of canine Ad-MSCs. Freshly isolated canine Ad-MSCs from 3 additional donors (donors 4–6) were placed directly into serum-free medium containing bFGF ...
Lynne Boxer (6407117), Laxminarayana R. Devireddy (6407108), Rudell Screven (6407111), Michael Myers (3954902), Zhuoming Liu (6407114)   +4 more
core   +1 more source

Exploring ethical, sustainable and effective foetal bovine serum alternatives for in vitro mammalian cell culture

Frontiers in Toxicology
Foetal bovine serum (FBS) has been widely used as a nutrient-rich supplement in mammalian cell culture for over 6 decades; however, its usage has increasingly raised various concerns and challenges related to quality variations, unethical collection ...
Huiyuan Meng, Philip J. R. Day, Philip J. R. Day, Philip J. R. Day   +3 more
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Platelet lysate as a serum substitute for 2D static and 3D perfusion culture of stromal vascular fraction cells from human adipose tissue [PDF]

, 2009
Fetal bovine serum (FBS) and fibroblast growth factor (FGF)-2 are key supplements for the culture of stromal vascular fraction (SVF) cells from adipose tissue, both for typical monolayer (2D) expansion and for streamlined generation of osteogenic ...
Alini, Mauro, Verrier, Sophie, Droeser, Raoul, Bocelli-Tyndall, Chiara, Davenport, Michael, Martin, Ivan, Scherberich, Arnaud, Müller, Andreas M., Schaefer, Dirk J.   +8 more
core  

Serum-free media for articular chondrocytes in vitro expansion

Chinese Medical Journal, 2013
Background In vitro chondrocyte expansion is a major challenge in cell-based therapy for human articular cartilage repair. Classical culture conditions usually use animal serum as a medium supplement, which raises a number of undesirable questions.
Xin-xin, Shao, Neil A, Duncan, Lin, Lin, Xin, Fu, Ji-ying, Zhang, Chang-long, Yu   +5 more
openaire   +2 more sources

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source

Censorship in Australia: Intrusions into media freedom flying beneath the international free expression radar

, 2015
Australia has ranked among the top 30 nations in recent world press freedom surveys published by Reporters Without Borders (RSF) and Freedom House and is broadly regarded as a substantially free Western liberal democracy.
Pearson, Mark, Fernandez, Joseph M
core   +2 more sources