Results 51 to 60 of about 730,822 (283)

Performance of serum-free broth media for growth of Renibacterium salmoninarum [PDF]

open access: yesDiseases of Aquatic Organisms, 1998
Growth of Renibacterium salmoninarum was compared in 14 different broth media; 13 serum-free, and 1 that contained newborn calf serum, KDM2+M. Supplementation with 1% v/v R. salmoninarum MCO4M metabolite was evaluated for 6 of the media that do not utilize it as part of their ingredients.
C E, Starliper, W B, Schill, J, Mathias
openaire   +2 more sources

Epigenetic blind spots – the role of DNA methylation dynamics in stem cell‐based models of embryogenesis

open access: yesFEBS Letters, EarlyView.
Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil   +4 more
wiley   +1 more source

Investigating the mass of Soya Bean Kazein which has the same content of protein with 2,7g Tryptose Phosphate Broth [PDF]

open access: yes, 2011
Cell cultures are used rather frequently due to its being economic, easy to control and allowing industrial products to yield. The most common area of usage of cell culture is the vaccine production and vaccine of Foot and Mouth Disease is the one of ...
Cengiz, Göksu
core  

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

open access: yesFEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai   +4 more
wiley   +1 more source

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

open access: yesFEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Serum-Free Cryopreservation of Five Mammalian Cell Lines in Either a Pelleted or Suspended State

open access: yesBiological Procedures Online, 2004
Herein we have explored two practical aspects of cryopreserving cultured mammalian cells during routine laboratory maintenance. First, we have examined the possibility of using a serum-free, hence more affordable, cryopreservative.
Corsini Joe   +2 more
doaj   +1 more source

PARK(ing) time–How park deficiency affects the biological clock in a Drosophila model of Parkinson's disease

open access: yesFEBS Letters, EarlyView.
Drosophila park mutants serve as a model for Parkinson's disease. We used this strain to investigate the connection between oxidative stress and the circadian clock mechanism. We showed that increased oxidative stress affects the physiology of pacemaker cells, disrupting their daily structural plasticity. Lack of rhythmic signaling from pacemaker cells
Kamila Zientara   +3 more
wiley   +1 more source

Comparison of CD34+ cell growth, differentiation and transduction in serum vs serum-free media.

open access: yes, 2013
(A) Cell expansion and viability after 4 days in culture in various media. Data collated from independent experiments (n = 3 for IMDM/FBS, Stemline and X-vivo 10; n = 2 for HPGM and CellGro; n = 1 for Stemspan), and normalized to IMDM/FBS control.
Sylvie Shen (371528)   +4 more
core   +1 more source

Serum-free long-term expansion and functional maintenance of pig satellite cells via targeted genetic engineering for cultured meat

open access: yesFood Materials Research
Cultured meat represents a promising alternative for future meat consumption. However, the progress of research and industrialization has been hindered by the absence of serum-free media capable of supporting the long-term expansion of specific cells ...
Renpeng Guo   +8 more
doaj   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

open access: yesFEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee   +7 more
wiley   +1 more source

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