Results 71 to 80 of about 730,822 (283)

Increasing sustainability and reproducibility of in vitro toxicology applications: serum-free cultivation of HepG2 cells

Frontiers in Toxicology
Fetal Bovine Serum (FBS) is an important ingredient in cell culture media and the current standard for most cells in vitro. However, the use of FBS is controversial for several reasons, including ethical concerns, political, and societal pressure, as ...
Luisa Marie Pfeifer, Janike Sensbach, Frederic Pipp, Daniela Werkmann, Philip Hewitt   +4 more
doaj   +1 more source

Vasculogenesis from Human Dental Pulp Stem Cells Grown in Matrigel with Fully Defined Serum-Free Culture Media

Biomedicines, 2020
The generation of vasculature is one of the most important challenges in tissue engineering and regeneration. Human dental pulp stem cells (hDPSCs) are some of the most promising stem cell types to induce vasculogenesis and angiogenesis as they not only ...
Jon Luzuriaga, Jon Irurzun, Igor Irastorza, Fernando Unda, Gaskon Ibarretxe, Jose R. Pineda   +5 more
doaj   +1 more source

Engineered extracellular vesicles enriched with the miR‐214/199a cluster enhance the efficacy of chemotherapy in ovarian cancer

Molecular Oncology, EarlyView.
Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang, Ayesha Alvero, Yi Qin, Mingjin Wang, Alexandra Fox, Yanfeng Li, Michael Millman, Amy Kemper, Gil Mor, Xian Shuang Liu, Michael Chopp, Zheng Gang Zhang, Yi Zhang   +12 more
wiley   +1 more source

Attachment promoting compounds significantly enhance cell proliferation and purity of bovine satellite cells grown on microcarriers in the absence of serum

Frontiers in Bioengineering and Biotechnology
IntroductionTo bring cultivated beef to the market, a scalable system that can support growth of bovine satellite cells (bSCs) in a serum-free and preferably also animal-free medium is of utmost importance.
Vincent Bodiou, Vincent Bodiou, Anitha Ajith Kumar, Edoardo Massarelli, Tessa van Haaften, Mark J. Post, Mark J. Post, Panagiota Moutsatsou   +7 more
doaj   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Keratin 19 as a prognostic marker and contributing factor of metastasis and chemoresistance in high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Keratin 19 (KRT19) is overexpressed in high‐grade serous ovarian cancer with high levels of Kallikrein‐related peptidases (KLK) 4–7 and is associated with poor survival. In vivo analyses demonstrate that elevated KRT19 increases peritoneal tumour burden.
Sophia Bielesch, Isabel Vogel, Sina Nokodian, Johanna Moeller, Antonia Blechschmidt, Vicky Hecht, Vanessa Kuentzel, Katharina Thiedig, Melissa Schwab, Oliver Schilling, Holger Bronger, Marion Kiechle, Viktor Magdolen, Tobias Dreyer   +13 more
wiley   +1 more source

Serum-free freezing media support high cell quality and excellent ELISPOT assay performance across a wide variety of different assay protocols. [PDF]

, 2012
Robust and sensitive ELISPOT protocols are commonly applied concomitant with the development of new immunotherapeutics. Despite the knowledge that individual serum batches differ in their composition and may change properties over time, serum is still ...
Welters, Marij J.P., Filbert, Helene, Janetzki, Sylvia, Burg, Sjoerd H. van der, Ottensmeier, Christian, Britten, Cedrik M., Attig, Sebastian, Sahin, Ugur, Gouttefangeas, Cécile, Welters, Marij J. P., Bidmon, Nicole, van der Burg, Sjoerd H., Renard, Bernhard Y.   +12 more
core   +1 more source

Dual PI3K/AKT and CDK4/6 inhibition reveals selective sensitivity in an SHH medulloblastoma stem cell model

Molecular Oncology, EarlyView.
Targeted therapy was evaluated in SHH medulloblastoma using neuroepithelial stem cell (NES) and tumor‐derived NES‐like (tNES) models in 2D monolayers and 3D spheroids. PI3K, AKT, and CDK4/6 inhibitors had minimal effects in NES but markedly reduced viability and growth and induced apoptosis in tNES cells, revealing distinct therapeutic vulnerabilities.
Monika Lukoseviciute, Madeleine Birgersson, Paolo Ceriani, Margareta Wilhelm, Ourania N Kostopoulou   +4 more
wiley   +1 more source

Increased myelination efficiency by transplanted OPCs in older slices and serum-free media.

, 2013
Representative images comparing transplantation of CMFDA-labelled OPCs into 3 DIV organotypic slices cultured in serum-containing media (A–C), 16 DIV organotypic cerebellar slices cultured in serum-containing media (D–F), and 16 DIV organotypic ...
Sarah-Jane Bull (315171), Jenea M. Bin (315169), Jack P. Antel (315172), Timothy E. Kennedy (205904), Soo Yuen Leong (315170)   +4 more
core   +1 more source

Optimization of Culture Media for Human Umbilical Cord-Derived Mesenchymal Stem Cell Production

Stem Cells International
The development of robust and scalable culture systems is essential for the clinical-scale production of human umbilical cord (UC)-derived mesenchymal stem/stromal cells (MSCs) (UC-MSCs).
Wanglong Chu, Muyun Liu, Yan Shangguan, Fangtao He, Xiuping Zeng, Tao Guo, Tongjing Li, Fen Zhang, Qingfang Wang, Jianfu Wu, Zhenzhong Zhong, Xiao Liang   +11 more
doaj   +1 more source