Results 91 to 100 of about 730,822 (283)

AgNP suspension characterization in water or serum-free cell culture media.

, 2013
AgNP suspension characterization in water or serum-free cell culture media.
Frank A. Witzmann (430777), Ramakrishna Podila (456098), Jared M. Brown (456099), Pu Chun Ke (456097), Jonathan H. Shannahan (456095), Xianyin Lai (456096)   +5 more
core   +1 more source

Serum-and protein-free media formulations for the Chinese hamster ovary cell line DUKXB11 [PDF]

, 2004
The production of therapeutic proteins in mammalian cell lines is of outstanding importance. The maintenance of most mammalian cell lines in culture requires the addition of serum to the culture medium.
Matischak, K., Schröder, M., Friedl, P., Friedl, Peter   +3 more
core   +1 more source

Evaluation of the viability and functionality of human peripheral blood mononuclear cells cryopreserved up to 2 years in animal-protein-free freezing media compared to the FBS-supplemented reference medium

Frontiers in Immunology
BackgroundCryopreservation of peripheral blood mononuclear cells (PBMCs) is crucial for consistent analysis in immunological studies and clinical trials. Traditional freezing media often contain fetal bovine serum (FBS), which raises ethical concerns and
Frédérique Jantet-Blaudez, Joseline Ruiz, Sylviane Gautheron, Anke Pagnon   +3 more
doaj   +1 more source

Heterozygous loss‐of‐function alleles associate the conserved 3′‐5′ exoribonuclease EXOSC10 with hypersensitivity to the anticancer drug 5‐fluorouracil

Molecular Oncology, EarlyView.
EXOSC10, an essential nuclear RNA exosome‐associated 3′‐5′ exoribonuclease, is inhibited by the anticancer drug 5‐fluorouracil (5‐FU), and EXOSC10 depletion increases 5‐FU sensitivity. The colon‐cancer variant EXOSC10S402T, located in a proteolysis motif, is stable and nuclear but nonfunctional in vivo.
Radhika Sain, Yann Le Page, Frédéric Percevault, Emmanuelle Becker, Luc Negroni, Almudena Fernandez, Marta Cantero, Diego Muñoz‐Santos, Lluís Montoliu, Cyrille Garnier, Michael Primig   +10 more
wiley   +1 more source

Particle size distribution in water and serum-free cell culture media.

, 2015
Particle size distribution in water and serum-free cell culture media.
Andrea Valsesia (820299), Isaac Ojea-Jiménez (820301), Sabrina Gioria (820302), François Rossi (559475), Agnieszka Kinsner-Ovaskainen (742576), Mara Ceridono (820297), Blanka Halamoda-Kenzaoui (820296), Pascal Colpo (820298), Patricia Urbán (820300), Douglas Gilliland (559473)   +9 more
core   +1 more source

Cell‐cycle‐specific lesion evolution rather than inhibition of double‐strand‐break repair underpins cisplatin radiosensitization

Molecular Oncology, EarlyView.
We analyze cisplatin–DNA adducts (CDAs) and double‐strand breaks (DSBs) in a cell‐cycle‐dependent manner. We find that CDAs form similarly across all cell cycle phases. DSBs arise only in S‐phase. CDAs might not directly impair DSB repair, but S‐phase DSB lesions evolve in the presence of CDAs and disrupt repair in G2, also causing radiosensitization ...
Ye Qiu, Xixi Lin, Emil Mladenov, Veronika Mladenova, Jürgen Thomale, Ali Sak, Yao Wang, Yunxuan Deng, Eleni Gkika, Martin Stuschke, George Iliakis   +10 more
wiley   +1 more source

Performance of serum-supplemented and serum-free media in IFN gamma Elispot Assays for human T cells

, 2010
The choice of serum for supplementation of media for T cell assays and in particular, Elispot has been a major challenge for assay performance, standardization, optimization, and reproducibility.
Janetzki, S., Hayes, P., Currier, J.R., Kaempgen, E., Souza, V., Caterini, J., Lennerz, V., Price, L., Ferrari, G., Britten, C.M., Hoos, A., Burg, S.H. van der, Gouttefangeas, C., Nihlmark, K.   +13 more
core  

Neuroblastoma cells form tumorspheres in serum-free media.

, 2013
(A) LA-N-5 neuroblastoma cells plated in serum supplemented media show adherent growth while those plated in serum-free media (with EGF and bFGF) formed non-adherent tumorspheres. (B) Dissociated bulk and tumorsphere cells were subjected to FACS analysis.
Nancy Ratner (375065), Yoshinaga Saeki (262986), Jon P. Williams (221752), Timothy P. Cripe (221759), Jonathan Grossheim (375063), Jose A. Cancelas (375064), William H. Baird (375061), Bryan Mitton (375062), Yonatan Y. Mahller (375060)   +8 more
core   +1 more source

Identification of albumin-bound fatty acids as the major factor in serum-induced lipid accumulation by cultured cells

Journal of Lipid Research, 1970
Factors responsible for the high lipogenic activity of rabbit serum were investigated using an assay procedure based on the gravimetric determination of the 24 hr increase in cell lipid.
COSMO G. MACKENZIE, JULIA B. MACKENZIE, OSCAR K. REISS, JUDITH A. WISNESKI   +3 more
doaj   +1 more source

Development of serum-free media for the culture and transfer of human blastocysts [PDF]

Human Reproduction, 1998
The culture and transfer of the blastocyst stage embryo has several advantages for assisted reproduction in the human. However, due to inadequacies of present culture conditions in human in-vitro fertilization (IVF), embryos are routinely transferred to the uterus on either day 2 or day 3 of development around the 4- to 8-cell stage, with resultant ...
openaire   +2 more sources