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Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assays [PDF]
Frontiers in ToxicologyThe replacement, reduction, and refinement (3Rs) of animal experiments is a central objective in modern toxicology. Human cell-based in vitro assays have become key tools to implement these principles by providing mechanistically driven and human ...
Julia Vanessa Spänle +5 more
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The effect of macromolecular crowders as a supplement to serum free media on human corneal stromal cells proliferation and marker expression [PDF]
Scientific ReportsNative human corneal stromal keratocytes (HCSKs) deposit extracellular matrix (ECM) to maintain corneal homeostasis, repair, and regeneration. Adding macromolecular crowders (MMCs) to culture media encourages HCSKs to produce ECM in vitro.
Weaam Ahmed Sultan, Che J. Connon
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Multi-response optimization of defined and undefined serum-free media for enhanced proliferation of immortalized lamb muscle cells [PDF]
Scientific ReportsThe development of serum-free media (SFM) is critical for advancing the scalability and ethical viability of cultivated meat. To our knowledge, this study represents the first systematic, species-specific optimization of serum-free media for immortalized
Arian Amirvaresi, Reza Ovissipour
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Future Foods, 2023 Successful commercialization of cultivated meat products as alternative sources of dietary protein for a growing global population will require development of efficient and inexpensive serum-free cell culture media.
Edward N. O'Neill +6 more
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Supply Chain Disruptions during COVID-19 Pandemic Uncover Differences in Keratinocyte Culture Media
JID Innovations, 2022 Various culture media are used to propagate keratinocytes (KCs) in vitro. The COVID-19 pandemic resulted in supply chain shortages necessitating substitutions to standard laboratory protocols, which resulted in many laboratories having to use culture ...
Mary C. Moran +3 more
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Posology and Serum-/Xeno-Free Engineered Adipose Stromal Cells Cell Sheets
Frontiers in Cell and Developmental Biology, 2022 Well-characterized adipose stem cells and chemically defined culture media are important factors that control the production of the cell sheet, used in translational medicine.
Jun Ochiai +4 more
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Frontiers in Veterinary Science, 2022 Fetal bovine serum (FBS) remains widely used as a supplement in cell culture media used in the isolation and expansion of mesenchymal stromal cells (MSC) despite longstanding practical, clinical, and ethical concerns over its use.
Cara R. Pilgrim +5 more
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PLoS ONE, 2013 Primary human epidermal stem cells isolated from skin tissues and subsequently expanded in tissue culture are used for human therapeutic use to reconstitute skin on patients and to generate artificial skin in culture for academic and commercial research.
Rebecca Lamb, Carrie A Ambler
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Biomedicines, 2020 The generation of vasculature is one of the most important challenges in tissue engineering and regeneration. Human dental pulp stem cells (hDPSCs) are some of the most promising stem cell types to induce vasculogenesis and angiogenesis as they not only ...
Jon Luzuriaga +5 more
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Serum-free cell culture: the serum-free media interactive online database
ALTEX, 2010 Fetal bovine serum (FBS) is a ubiquitously used essential supplement in cell culture media. However, there are serious scientific and ethical concerns about the use of FBS regarding its harvest and production. During the last three decades, FBS could be substituted by other supplements or by the use of defined chemical components in serum-free cell ...
Daniel, Brunner +5 more
openaire +3 more sources