Results 31 to 40 of about 437,976 (307)

Effect of Pre-Incubation of Electrospun Silk Fibroin Scaffold in Complete and Serum-Free Media on Survival and Proliferation of Rat Bone Marrow Mesenchymal Stem Cells

Medical Laboratory Journal, 2022
Background and objectives: Developing scaffolds is important for tissue engineering and repairing damaged tissues. The present study aimed to investigate effects of pre-incubation of an electrospun silk fibroin scaffold in complete and serum-free media ...
Maryam Janitermi, esmail fattahi, seyed Gholam ali Jorsaraei   +2 more
doaj  

Effect of Epidermal Growth Factor on In Vitro Maturation, Fertilization and Early Embryonic Development of Cattle Oocytes

International Journal of Bio-Resource and Stress Management
The present experiment was conducted during January, 2018 to March, 2019 in the laboratory, Dept. of Veterinary Physiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam (781022), India to investigate the ...
Dipannita Baishya, Arundhati Bora, Champak Barman   +2 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Fibroblastic Transformation of Corneal Keratocytes by Rac Inhibition is Modulated by Extracellular Matrix Structure and Stiffness

Journal of Functional Biomaterials, 2015
The goal of this study was to investigate how alterations in extracellular matrix (ECM) biophysical properties modulate corneal keratocyte phenotypes in response to specific wound healing cytokines and Rho GTPases.
W. Matthew Petroll, Neema Lakshman
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Cultures Through Time: Forging a Xeno-Free Future for Cell Culture-Based Virology

Vaccines
As a cornerstone of modern science, cell lines are the foundational platforms for key medical advances. They enable vaccinology (through viral propagation and attenuation), gene therapy (via vector development), and biopharmaceutical production (via ...
Arvind Singh Kaulsay, Nurshariza Abdullah, Nur Amelia Azreen Adnan   +2 more
doaj   +1 more source

Cultivation, cryopreservation and resuscitation of Theileria annulata transformed cells in serum-free media

Frontiers in Veterinary Science, 2022
IntroductionTropical theileriosis is a protozoan disease caused by Theileria annulata that affects cattle in Northern Africa, the Middle East and Asia where vector ticks of the genus Hyalomma occur.
Khawla Elati, Khawla Elati, Khawla Elati, Erich Zweygarth, Erich Zweygarth, Moez Mhadhbi, Mohamed Aziz Darghouth, Ard M. Nijhof, Ard M. Nijhof   +8 more
doaj   +1 more source

Platelet lysate as a serum substitute for 2D static and 3D perfusion culture of stromal vascular fraction cells from human adipose tissue [PDF]

, 2009
Fetal bovine serum (FBS) and fibroblast growth factor (FGF)-2 are key supplements for the culture of stromal vascular fraction (SVF) cells from adipose tissue, both for typical monolayer (2D) expansion and for streamlined generation of osteogenic ...
Alini, Mauro, Verrier, Sophie, Droeser, Raoul, Bocelli-Tyndall, Chiara, Davenport, Michael, Martin, Ivan, Scherberich, Arnaud, Müller, Andreas M., Schaefer, Dirk J.   +8 more
core  

Serum-free medium containing bFGF and PDGF facilitates isolation and expansion of canine Ad-MSCs.

, 2019
A. Schematic to evaluate serum-free medium containing bFGF and PDGF for direct derivation and expansion of canine Ad-MSCs. Freshly isolated canine Ad-MSCs from 3 additional donors (donors 4–6) were placed directly into serum-free medium containing bFGF ...
Lynne Boxer (6407117), Laxminarayana R. Devireddy (6407108), Rudell Screven (6407111), Michael Myers (3954902), Zhuoming Liu (6407114)   +4 more
core   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source