Results 51 to 60 of about 437,976 (307)

Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells [PDF]

, 2017
Wnt signalling proteins are essential for culture of human organ stem cells in organoids, but most Wnt protein formulations are poorly active in serum-free media.
Hui, Lijian, Clevers, Hans, Pharmaceutics, Cornelissen, Jan J., Vries, Robert, Hans Clevers, van den Brink, Stieneke, van der Laan, Luc J W, Cornelissen, Dirk-Jan, Begthel, Harry, van der Laan, Luc J. W., Louis van Bloois, Harry Begthel, Verstegen, Monique M.A.; id_orcid, Luis J. Cruz, Nesrin Tüysüz, Vries, Robert R G, De Jonge, Jeroen; id_orcid, de Jonge, Jeroen, Mastrobattista, Enrico, van Bloois, Louis, Van Der Laan, Luc J.W., Stieneke van den Brink, Lijian Hui, Ten Berge, Derk, Eric Braakman, Enrico Mastrobattista, Derk ten Berge, Verstegen, Monique M.A., Robert Vries, Cruz, Luis J, Cornelissen, Jan J, Braakman, Eric, Luc J. W. van der Laan, Monique M. A. Verstegen, Jeroen de Jonge, Cruz, Luis J., Van Der Laan, Luc J.W.; id_orcid, Tüysüz, Nesrin, Jan J. Cornelissen, Afd Pharmaceutics, Verstegen, Monique M. A., Verstegen, Monique M A   +42 more
core   +3 more sources

Optimization of Culture Media for Human Umbilical Cord-Derived Mesenchymal Stem Cell Production

Stem Cells International
The development of robust and scalable culture systems is essential for the clinical-scale production of human umbilical cord (UC)-derived mesenchymal stem/stromal cells (MSCs) (UC-MSCs).
Wanglong Chu, Muyun Liu, Yan Shangguan, Fangtao He, Xiuping Zeng, Tao Guo, Tongjing Li, Fen Zhang, Qingfang Wang, Jianfu Wu, Zhenzhong Zhong, Xiao Liang   +11 more
doaj   +1 more source

Increasing sustainability and reproducibility of in vitro toxicology applications: serum-free cultivation of HepG2 cells

Frontiers in Toxicology
Fetal Bovine Serum (FBS) is an important ingredient in cell culture media and the current standard for most cells in vitro. However, the use of FBS is controversial for several reasons, including ethical concerns, political, and societal pressure, as ...
Luisa Marie Pfeifer, Janike Sensbach, Frederic Pipp, Daniela Werkmann, Philip Hewitt   +4 more
doaj   +1 more source

A study of antibody secretion from hybridoma cells [PDF]

, 1988
The secretion of monoclonal antibody in both batch and continuous cultures was studied. It was found that when hybridoma cells were continuously cultivated in a simple lab scale fermenter, they secreted less antibody than cells grown in stationary ...
Holland, P.N.
core  

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Low calcium levels in serum-free media maintain chondrocyte phenotype in monolayer culture and reduce chondrocyte aggregation in suspension culture

, 2005
SummaryObjectiveExtracellular calcium influences chondrocyte differentiation and synthesis of extracellular matrix. Previously, calcium concentrations ranging from 0.1mM to 2mM have been used in vitro and these studies indicated that low calcium ...
Gigout, A., A. Gigout, M. Jolicoeur, M.D. Buschmann, Jolicoeur, Mario, Jolicoeur, M., Buschmann, M.D., Buschmann, Michael D.   +7 more
core   +1 more source

Serum-free alginate-C2C12 cells microcapsule culture as an alternative source of animal protein

, 2023
Due to the climate change crisis, and environmental impacts of the traditional meat sector, the production of artificial animal protein based on in vitro cell culture technology is proposed as an alternative.
Bruheim, Per, Jang, Mi, Scheffold, Jana, Kjesbu, Joachim Sebastian   +3 more
core   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Development of serum-free media for lepidopteran insect cell lines

, 2007
Lepidopteran insect cell culture technology has progressed to the point of becoming an essential part of one of the most successful eukaryotic expression systems and is increasingly used industrially on a large scale.
Spiros N. Agathos, Agathos, Spiros N.
core   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source