Results 71 to 80 of about 437,976 (307)

Selection of medium for serum-free primary culture of adult rat hepatocytes.

, 1990
To select a suitable medium for serum-free primary culture of adult rat hepatocytes, ten commercially-available synthetic media were compared for their ability to maintain the cells under serum-free and serum-supplemented conditions with special ...
Sato, Jiro, Miyazaki, Masahiro, Bai, Liyan   +2 more
core   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Serum- and xeno-free culture of human umbilical cord perivascular cells for pediatric heart valve tissue engineering

Stem Cell Research & Therapy, 2023
Background Constructs currently used to repair or replace congenitally diseased pediatric heart valves lack a viable cell population capable of functional adaptation in situ, necessitating repeated surgical intervention.
Shouka Parvin Nejad, Monica Lecce, Bahram Mirani, Nataly Machado Siqueira, Zahra Mirzaei, J. Paul Santerre, John E. Davies, Craig A. Simmons   +7 more
doaj   +1 more source

Keratin 19 as a prognostic marker and contributing factor of metastasis and chemoresistance in high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Keratin 19 (KRT19) is overexpressed in high‐grade serous ovarian cancer with high levels of Kallikrein‐related peptidases (KLK) 4–7 and is associated with poor survival. In vivo analyses demonstrate that elevated KRT19 increases peritoneal tumour burden.
Sophia Bielesch, Isabel Vogel, Sina Nokodian, Johanna Moeller, Antonia Blechschmidt, Vicky Hecht, Vanessa Kuentzel, Katharina Thiedig, Melissa Schwab, Oliver Schilling, Holger Bronger, Marion Kiechle, Viktor Magdolen, Tobias Dreyer   +13 more
wiley   +1 more source

Serum‐Free Japanese Encephalitis Virus Production in a Single‐Use Fixed‐Bed Bioreactor

Engineering in Life Sciences
Fixed‐bed bioreactors for anchorage‐dependent cells are an obvious choice for development because of their large‐scale capabilities, allowing manufacturing with reduced cost and footprint.
Marco Kress, Robert Schlegl, Alois Jungbauer   +2 more
doaj   +1 more source

Chitosan Rate of Uptake in HEK293 Cells is Influenced by Soluble versus Microparticle State and Enhanced by Serum-Induced Cell Metabolism and Lactate-Based Media Acidification

Molecules, 2013
Chitosan is a biocompatible polysaccharide composed of glucosamine and N-acetylglucosamine. The polymer has a unique behavior of fluctuating between soluble chains at pH 6 and insoluble microparticles at pH 7.
Marc Lavertu, Mario Jolicoeur, Geneviève Lavallée, Nicolas Tran-Khanh, Jessica Guzmán-Morales, Caroline D. Hoemann   +5 more
doaj   +1 more source

Serum-free culturing of mammalian cells - adaptation to and cryopreservation in fully defined media

ALTEX, 2007
Long term storage of living cells is a central issue in cell biology and medicine. In addition to the cryoprotectant dimethyl sulphoxide (DMSO), foetal bovine serum (FBS) is often added to the freezing medium for the cryoconservation of serum dependent cell lines.
González Hernández Y, Fischer RW
openaire   +3 more sources

Upregulation of Adipogenesis and Chondrogenesis in MSC Serum-Free Culture

, 2011
Serum-free media have been shown to be effective in the expansion of mesenchymal stem cells (MSCs). However, the effects may go beyond cell expansion as the differentiation potentials of the cells may be modified, thus influencing their efficacy for ...
Vivek Madhukar Tanavde, Eng Hin Lee, James Hoi Hui, Saey Tuan Barnabas Ho   +3 more
core   +1 more source

Children and New Media: Youth Media Participation. A Case Study of Egypt and Finland [PDF]

, 2011
This article focuses on a single case study; the first findings of a qualitative part of the Youth Media Participation (YMP) project in Argentina, Egypt, Finland and India (2009-11) on 10-18 year-old children’s participation through media.
Tayie, Sami, Tayie, Samy, Hirsjärvi, Irma, Sami Tayie, Irma Hirsjärvi   +4 more
core   +1 more source

CCDC80 suppresses high‐grade serous ovarian cancer migration via negative regulation of B7‐H3

Molecular Oncology, EarlyView.
PAX8 is a lineage‐specific master regulator of transcription in high‐grade serous ovarian cancer (HGSC) progression. We show for the first time that PAX8 facilitates proliferation and metastasis by repressing the cell autonomous tumor suppressor CCDC80 and inducing B7‐H3 expression.
Aya Saleh, Nitzan Medina‐Itzhaki, Milena Chekov, Eden Gal‐Swisa, Roba Gabesh‐Wahabi, Inbar Savyon, Inna Naroditsky, Hilary A. Kenny, Basem Fares, Lina Korsensky, Einav Girsh, Liron Berger, Ruth Perets   +12 more
wiley   +1 more source