Results 21 to 30 of about 437,976 (307)

Development of serum-free media for the culture and transfer of human blastocysts [PDF]

Human Reproduction, 1998
The culture and transfer of the blastocyst stage embryo has several advantages for assisted reproduction in the human. However, due to inadequacies of present culture conditions in human in-vitro fertilization (IVF), embryos are routinely transferred to the uterus on either day 2 or day 3 of development around the 4- to 8-cell stage, with resultant ...
openaire   +2 more sources

The necessity for standardization of glioma stem cell culture: a systematic review

Stem Cell Research & Therapy, 2020
Background The cancer stem cell hypothesis is an old idea which has been revived in recent years for many cancers, including gliomas. However, this concept has become controversial due to a series of studies with conflicting results. Methods A systematic
Lei Zhang, Hongwei Yu, Yuhui Yuan, John S. Yu, Zhenkun Lou, Yixue Xue, Yunhui Liu   +6 more
doaj   +1 more source

In vitro culture of bovine fibroblasts using select serum-free media supplemented with Chlorella vulgaris extract

BMC Biotechnology, 2023
Standard cell culture practices require the addition of animal-derived serum to culture media to achieve adequate cell growth. Typically, 5–10% by volume of fetal bovine serum (FBS) is used, which accounts for a vast majority of the media cost while also
Galileo Defendi-Cho, Timothy M. Gould
doaj   +1 more source

Optimization of serum-free fermentation processes for antibody production

, 1991
Büntemeyer H, Lütkemeyer D, Lehmann J. Optimization of serum-free fermentation processes for antibody production. Cytotechnology. 1991;5(1):57-67.Serum free fermentation procedures of cell cultures have got a wide application in production of ...
Lehmann, Jürgen, Büntemeyer, Heino, Lütkemeyer, Dirk   +2 more
core   +2 more sources

Comparing the immunomodulatory properties of equine BM-MSCs culture expanded in autologous platelet lysate, pooled platelet lysate, equine serum and fetal bovine serum supplemented culture media

Frontiers in Veterinary Science, 2022
Joint injury often leads to cartilage damage and posttraumatic inflammation, which drives continued extracellular matrix degradation culminating in osteoarthritis.
Kayla M. Even, Angela M. Gaesser, Sarah A. Ciamillo, Renata L. Linardi, Kyla F. Ortved   +4 more
doaj   +1 more source

Design of a bubble-swarm bioreactor for animal cell culture

, 1994
Gudermann F, Lütkemeyer D, Lehmann J. Design of a bubble-swarm bioreactor for animal cell culture. Cytotechnology. 1994;15(1-3):301-309.A stationary bubble-swarm has been used to aerate a mammalian cell culture bioreactor with an extremely low gas flow ...
Lehmann, Jürgen, Gudermann, F., Lütkemeyer, Dirk   +2 more
core   +2 more sources

The impact of serum-free culture on HEK293 cells: From the establishment of suspension and adherent serum-free adaptation cultures to the investigation of growth and metabolic profiles [PDF]

, 2022
Serum-free cultures are preferred for application in clinical cell therapy and facilitate the purification processes of bioproducts, such as vaccines and recombinant proteins.
Bruheim, Per, Pete, Ellen Sofie, Jang, Mi   +2 more
core   +1 more source

Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization

Stem Cells International, 2017
To achieve safer patient treatments, serum-free cell culture conditions have to be established for cell therapies. In previous studies, we demonstrated that serum-free culture favored the proliferation of MSCA-1+ osteoprogenitors derived from the jaw ...
Eva Brauchle, Daniel Carvajal Berrio, Melanie Rieger, Katja Schenke-Layland, Siegmar Reinert, Dorothea Alexander   +5 more
doaj   +1 more source

Influence of cell type and cell culture media on the propagation of foot-and-mouth disease virus with regard to vaccine quality

Virology Journal, 2018
Background Suspension culture of BHK cells allows large-scale virus propagation and cost-efficient vaccine production, while the shift to animal-component-free cell culture media without serum is beneficial for the quality and downstream processing of ...
Veronika Dill, Bernd Hoffmann, Aline Zimmer, Martin Beer, Michael Eschbaumer   +4 more
doaj   +1 more source

In vitro generation of monocyte-derived macrophages under serum-free conditions improves their tumor promoting functions. [PDF]

PLoS ONE, 2012
The tumor promoting role of M2 macrophages has been described in in vivo models and the presence of macrophages in certain tumor types has been linked to a poor clinical outcome.
Flora Rey-Giraud, Mathias Hafner, Carola H Ries   +2 more
doaj   +1 more source