Results 11 to 20 of about 437,976 (307)
Stem Cell Research & TherapyBackground Cell culture media are essential for cell expansion and many cells still depend on blood-derived supplements for optimal growth. From a regulatory perspective, these materials are ideally xeno-free, serum-free or even chemically defined ...
Willem Delabie +5 more
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In vitro cytotoxicity testing of airborne formaldehyde collected in serum-free culture media [PDF]
Toxicology and Industrial Health, 2005 The purpose of this study was to identify a suitable sampling model for on-site toxicity assessment of soluble air contaminants such as formaldehyde, a well known industrial and indoor air contaminant. The in vitro cytotoxicity of formaldehyde, the selected model for soluble air contaminants, was studied using the MTS (tetrazolium salt) assay in two ...
Khalil, C. +4 more
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Use of serum-free media for peripheral blood mononuclear cell culture and the impact on T and B cell readouts [PDF]
Frontiers in ToxicologyIntroductionAs part of a wider programme of work developing next-generation risk assessment approaches (NGRA) using non-animal methods (NAMs) for safety assessment of materials, Unilever SEAC is exploring the use of a peripheral blood mononuclear cell ...
Stella Cochrane +2 more
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Stem Cell Research & TherapyBackground The establishment of stable porcine embryonic stem cells (pESCs) can contribute to basic and biomedical research, including comparative developmental biology, as well as assessing the safety of stem cell-based therapies.
Hyerin Choi +11 more
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Applications and analysis of hydrolysates in animal cell culture
Bioresources and Bioprocessing, 2021 Animal cells are used in the manufacturing of complex biotherapeutic products since the 1980s. From its initial uses in biological research to its current importance in the biopharmaceutical industry, many types of culture media were developed: from ...
Yin Ying Ho +5 more
doaj +1 more source
Journal of Pharmacological Sciences, 2011 The aim of this study was to determine suitable culture conditions for maintaining the activity of cytochrome p450 (CYP) 3A4 and drug transporters in primary cultured human hepatocytes.
Yuko Takeba +8 more
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Culture of viable human blastocysts in defined sequential serum-free media [PDF]
Human Reproduction, 1998 In human in-vitro fertilization (IVF), embryos are routinely transferred to the uterus on either day 2 or day 3 of development, resulting in a 10-15% implantation rate. However, in other mammalian species, the transfer of cleavage stage embryos, which normally reside in the oviduct, to the uterus results in a significantly lower implantation rate ...
D K, Gardner, M, Lane
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Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium
Stem Cell Research & Therapy, 2019 Background Serum-free xenogen-free defined media and continuous controlled physiological cell culture conditions have been developed for stem cell therapeutics, but the effect of these conditions on the relative potency of the cell product is unknown. As
Seth Mount +11 more
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Serum-Free Cryopreservation of Five Mammalian Cell Lines in Either a Pelleted or Suspended State
Biological Procedures Online, 2004 Herein we have explored two practical aspects of cryopreserving cultured mammalian cells during routine laboratory maintenance. First, we have examined the possibility of using a serum-free, hence more affordable, cryopreservative.
Corsini Joe +2 more
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LIF-free embryonic stem cell culture in simulated microgravity. [PDF]
PLoS ONE, 2009 BackgroundLeukemia inhibitory factor (LIF) is an indispensable factor for maintaining mouse embryonic stem (ES) cell pluripotency. A feeder layer and serum are also needed to maintain an undifferentiated state, however, such animal derived materials need
Yumi Kawahara +5 more
doaj +1 more source