Results 31 to 40 of about 730,822 (283)

A Review of Fetal Bovine Serum in the Culture of Mesenchymal Stromal Cells and Potential Alternatives for Veterinary Medicine

Frontiers in Veterinary Science, 2022
Fetal bovine serum (FBS) remains widely used as a supplement in cell culture media used in the isolation and expansion of mesenchymal stromal cells (MSC) despite longstanding practical, clinical, and ethical concerns over its use.
Cara R. Pilgrim, Kiera A. McCahill, Jenna G. Rops, Jaustin M. Dufour, Keith A. Russell, Thomas G. Koch   +5 more
doaj   +1 more source

Serum-Free Suspension Adaptation of HEK-293T Cells: Basis for Large-Scale Biopharmaceutical Production

Brazilian Archives of Biology and Technology, 2021
Human Embryonic Kidney 293T cells (HEK-293T) are the most common host for viral vector production and are also widely employed for recombinant protein production.
Ana Luiza Oliveira Lomba, Mariane Cariati Tirapelle, Rafael Tagé Biaggio, Mário Soares Abreu-Neto, Dimas Tadeu Covas, Virginia Picanço-Castro, Kamilla Swiech, Amanda Mizukami   +7 more
doaj   +1 more source

Comparative Study of Culture Conditions for Maintaining CYP3A4 and ATP-Binding Cassette Transporters Activity in Primary Cultured Human Hepatocytes

Journal of Pharmacological Sciences, 2011
The aim of this study was to determine suitable culture conditions for maintaining the activity of cytochrome p450 (CYP) 3A4 and drug transporters in primary cultured human hepatocytes.
Yuko Takeba, Naoki Matsumoto, Sachiko Takenoshita-Nakaya, Yoshie Harimoto, Toshio Kumai, Yuichi Kinoshita, Hiroshi Nakano, Takehito Ohtsubo, Shinichi Kobayashi   +8 more
doaj   +1 more source

Optimization of serum-free fermentation processes for antibody production

, 1991
Büntemeyer H, Lütkemeyer D, Lehmann J. Optimization of serum-free fermentation processes for antibody production. Cytotechnology. 1991;5(1):57-67.Serum free fermentation procedures of cell cultures have got a wide application in production of ...
Lehmann, Jürgen, Büntemeyer, Heino, Lütkemeyer, Dirk   +2 more
core   +2 more sources

Chitosan Rate of Uptake in HEK293 Cells is Influenced by Soluble versus Microparticle State and Enhanced by Serum-Induced Cell Metabolism and Lactate-Based Media Acidification

Molecules, 2013
Chitosan is a biocompatible polysaccharide composed of glucosamine and N-acetylglucosamine. The polymer has a unique behavior of fluctuating between soluble chains at pH 6 and insoluble microparticles at pH 7.
Marc Lavertu, Mario Jolicoeur, Geneviève Lavallée, Nicolas Tran-Khanh, Jessica Guzmán-Morales, Caroline D. Hoemann   +5 more
doaj   +1 more source

Growth of v-src-transformed cells in serum-free medium through the induction of growth factors [PDF]

, 2012
The v-src oncogene is one of only two oncogenes capable of transforming mouse embryo fibroblasts (MEFs) lacking the IGF-IR gene (R-cells). R-/v-src cells grow robustly in the absence of serum, suggesting the hypothesis that they may produce one or more ...
Deangelis, Tiziana, Baserga, Renato, Quong, Andrew, Morrione, Andrea, Morrione|   +4 more
core   +1 more source

A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in the absence of serum starvation [PDF]

, 2022
Cultured meat production requires the robust differentiation of satellite cells into mature muscle fibres without the use of animal-derived components.
Dogan, A., Klevernic, I., Messmer, T., Furquim, C., Ovchinnikova, E., Flack, J.E., Cruz, H., Post, M.J.   +7 more
core   +1 more source

Design of a bubble-swarm bioreactor for animal cell culture

, 1994
Gudermann F, Lütkemeyer D, Lehmann J. Design of a bubble-swarm bioreactor for animal cell culture. Cytotechnology. 1994;15(1-3):301-309.A stationary bubble-swarm has been used to aerate a mammalian cell culture bioreactor with an extremely low gas flow ...
Lehmann, Jürgen, Gudermann, F., Lütkemeyer, Dirk   +2 more
core   +2 more sources

Effect of Epidermal Growth Factor on In Vitro Maturation, Fertilization and Early Embryonic Development of Cattle Oocytes

International Journal of Bio-Resource and Stress Management
The present experiment was conducted during January, 2018 to March, 2019 in the laboratory, Dept. of Veterinary Physiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam (781022), India to investigate the ...
Dipannita Baishya, Arundhati Bora, Champak Barman   +2 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source