Results 11 to 20 of about 730,822 (283)
Establishment of porcine embryonic stem cells in simplified serum free media and feeder free expansion [PDF]
Stem Cell Research & TherapyBackground The establishment of stable porcine embryonic stem cells (pESCs) can contribute to basic and biomedical research, including comparative developmental biology, as well as assessing the safety of stem cell-based therapies.
Hyerin Choi +11 more
doaj +3 more sources
Serum-Free Media and the Immunoregulatory Properties of Mesenchymal Stem Cells In Vivo and In Vitro
Cellular Physiology and Biochemistry, 2014 Background: Mesenchymal stem cells are capable of self-renewal and multi-lineage differentiation. They are used extensively to treat several diseases. Traditionally, mesenchymal stem cells are cultured in serum-containing media, typically supplemented ...
Mei Wu +8 more
doaj +2 more sources
A simple and robust serum-free media for the proliferation of muscle cells [PDF]
Food Research International, 2023 Cultivated meat production requires an efficient, robust and highly optimized serum-free cell culture media for the needed upscaling of muscle cell expansion. Existing formulations of serum-free media are complex, expensive and have not been optimized for muscle cells. Thus, we undertook this work to develop a simple and robust serum-free media for the
Stig Skrivergaard +2 more
exaly +3 more sources
Multi-response optimization of defined and undefined serum-free media for enhanced proliferation of immortalized lamb muscle cells [PDF]
Scientific ReportsThe development of serum-free media (SFM) is critical for advancing the scalability and ethical viability of cultivated meat. To our knowledge, this study represents the first systematic, species-specific optimization of serum-free media for immortalized
Arian Amirvaresi, Reza Ovissipour
doaj +2 more sources
Molecular Therapy: Methods & Clinical Development, 2018 In vitro erythroid differentiation from primary human cells is valuable to develop genetic strategies for hemoglobin disorders. However, current erythroid differentiation methods are encumbered by modest transduction rates and high baseline fetal ...
Naoya Uchida +6 more
doaj +2 more sources
Serum-free media supplements carry miRNAs that co-purify with extracellular vesicles [PDF]
Journal of Extracellular Vesicles, 2019 Recent studies on extracellular RNA raised awareness that extracellular vesicles (EVs) isolated from cultured cells may co-purify RNAs derived from media supplements such as fetal bovine serum (FBS) confounding EV-associated RNA.
Martin Auber +4 more
doaj +2 more sources
Journal of Ultrasonography, 2018 Objective: Moderately elevated level of thyroid-stimulating hormone accompanied by normal serum concentrations of free thyroxine, suggesting subclinical hypothyroidism, is the most common hormonal abnormality in obese children.
Małgorzata Rumińska +5 more
doaj +2 more sources
Stem Cell Research & TherapyBackground Cell culture media are essential for cell expansion and many cells still depend on blood-derived supplements for optimal growth. From a regulatory perspective, these materials are ideally xeno-free, serum-free or even chemically defined ...
Willem Delabie +5 more
doaj +2 more sources
Supply Chain Disruptions during COVID-19 Pandemic Uncover Differences in Keratinocyte Culture Media
JID Innovations, 2022 Various culture media are used to propagate keratinocytes (KCs) in vitro. The COVID-19 pandemic resulted in supply chain shortages necessitating substitutions to standard laboratory protocols, which resulted in many laboratories having to use culture ...
Mary C. Moran +3 more
doaj +1 more source
Extracellular small non-coding RNA contaminants in fetal bovine serum and serum-free media. [PDF]
Sci Rep, 2019 In the research field of extracellular vesicles (EVs), the use of fetal bovine serum (FBS) depleted of EVs for in vitro studies is advocated to eliminate the confounding effects of media derived EVs.
Mannerström B +5 more
europepmc +3 more sources