Results 21 to 30 of about 730,822 (283)

Posology and Serum-/Xeno-Free Engineered Adipose Stromal Cells Cell Sheets

open access: yesFrontiers in Cell and Developmental Biology, 2022
Well-characterized adipose stem cells and chemically defined culture media are important factors that control the production of the cell sheet, used in translational medicine.
Jun Ochiai   +4 more
doaj   +1 more source

Simplified Approaches for the Production of Monocyte-Derived Dendritic Cells and Study of Antigen Presentation in Bovine

open access: yesFrontiers in Veterinary Science, 2022
Dendritic cells are sentinels of the immune system responsible for the initiation of adaptive immune mechanisms. In that respect, the study of these cells is essential for a full understanding of host response to infectious agents and vaccines.
Patricia Cunha   +6 more
doaj   +1 more source

Evaluating the Potential of Marine Invertebrate and Insect Protein Hydrolysates to Reduce Fetal Bovine Serum in Cell Culture Media for Cultivated Fish Production

open access: yesBiomolecules, 2022
The use of fetal bovine serum (FBS) and the price of cell culture media are the key constraints for developing serum-free cost-effective media. This study aims to replace or reduce the typical 10% serum application in fish cell culture media by applying ...
Inayat Batish   +3 more
doaj   +1 more source

Cell-Based Fish: A Novel Approach to Seafood Production and an Opportunity for Cellular Agriculture

open access: yesFrontiers in Sustainable Food Systems, 2019
Cellular agriculture is defined as the production of agricultural products from cell cultures rather than from whole plants or animals. With growing interest in cellular agriculture as a means to address public health, environmental, and animal welfare ...
Natalie Rubio   +5 more
doaj   +1 more source

Development of a Serum-Free Medium To Aid Large-Scale Production of Mycoplasma-Based Therapies

open access: yesMicrobiology Spectrum, 2023
To assist in the advancement of the large-scale production of safe Mycoplasma vaccines and other Mycoplasma-based therapies, we developed a culture medium free of animal serum and other animal components for Mycoplasma pneumoniae growth.
Raul Burgos   +4 more
doaj   +1 more source

The role of recombinant proteins in the development of serum-free media [PDF]

open access: yesCytotechnology, 2006
Early developments in serum-free media led to a variety of formulations in which components normally provided in serum and required for growth (insulin, transferrin, lipid supplements, trace elements) and poorly defined components (extracts, hydrolysates) were added to defined basal media. These additives were mostly animal-derived.
Joanne, Keenan   +2 more
openaire   +2 more sources

Keratinocytes propagated in serum-free, feeder-free culture conditions fail to form stratified epidermis in a reconstituted skin model.

open access: yesPLoS ONE, 2013
Primary human epidermal stem cells isolated from skin tissues and subsequently expanded in tissue culture are used for human therapeutic use to reconstitute skin on patients and to generate artificial skin in culture for academic and commercial research.
Rebecca Lamb, Carrie A Ambler
doaj   +1 more source

Serum-free lentiviral vector production is compatible with medium-resident nuclease activity arising from adherent HEK293T host cells engineered with a nuclease-encoding transgene

open access: yesHeliyon, 2023
At present lentiviral vector production for cell and gene therapy commonly involves transient plasmid transfection of mammalian cells cultivated in serum-containing media and addition of exogenous nuclease to reduce host cell and plasmid DNA impurities ...
Sadfer Ali   +5 more
doaj   +1 more source

Serum-free cell culture: the serum-free media interactive online database

open access: yesALTEX, 2010
Fetal bovine serum (FBS) is a ubiquitously used essential supplement in cell culture media. However, there are serious scientific and ethical concerns about the use of FBS regarding its harvest and production. During the last three decades, FBS could be substituted by other supplements or by the use of defined chemical components in serum-free cell ...
Daniel, Brunner   +5 more
openaire   +3 more sources

In vitro development of Cryptosporidium parvum in serum-free media [PDF]

open access: yesLetters in Applied Microbiology, 2007
To compare commercially available serum-free media with common, standard, growth medium for their ability to support growth of Cryptosporidium parvum in HCT-8 cell cultures.Twelve serum-free media formulations with or without additional supplements were tested against a standard growth medium containing 2% FBS in HCT-8 cell cultures.
K M, Woods, S J, Upton
openaire   +2 more sources

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