Results 141 to 150 of about 8,999,056 (423)

AAV2/8 vectors purified from culture medium with a simple and rapid protocol transduce murine liver, muscle, and retina efficiently.

Human Gene Therapy Methods, 2013
During the production of some adeno-associated virus (AAV) serotypes, a large amount of vectors is found in the medium of producing cells. For their purification, previous protocols used tangential flow filtration (TFF) of the medium followed by ...
M. Dòria, Antonella Ferrara, A. Auricchio   +2 more
semanticscholar   +1 more source

Proteomics as a quality control tool of pharmaceutical probiotic bacterial lysate products [PDF]

, 2013
Probiotic bacteria have a wide range of applications in veterinary and human therapeutics. Inactivated probiotics are complex samples and quality control (QC) should measure as many molecular features as possible.
A Albalat, A Thomsen, AC Ouwehand, AV Simionato, Benoit Foligne, BGVV Arbeitsgruppe, C Santini, D Theodorescu, DM Carty, DM Good, E Dehlink, E Schiffer, F Gaggia, F Indrio, Günter Klein, H Mischak, H Mischak, Harald Mischak, J Hoffmann, J Jantos-Siwy, J Jähne, J Siwy, Janosch Hoffmann, JM Hamilton-Miller, Joost P. Schanstra, Justyna Siwy, K Savijoki, Kurt Zimmermann, L Sanchez-Hernandez, L Zhang, M Cazzola, MC Collado, ME Sanders, N Neuhoff, P Enck, P Hommerson, P Zürbig, P Zürbig, PM Sherman, R Haselberg, RGK Leuschner, S Lau, S Wittke, T Giese, T Volz, T Volz, Y Ohashi, Y Sun   +47 more
core   +3 more sources

Combined spatially resolved metabolomics and spatial transcriptomics reveal the mechanism of RACK1‐mediated fatty acid synthesis

Molecular Oncology, Volume 19, Issue 6, Page 1668-1686, June 2025.
The authors analyzed the spatial distributions of gene and metabolite profiles in cervical cancer through spatial transcriptomic and spatially resolved metabolomic techniques. Pivotal genes and metabolites within these cases were then identified and validated.
Lixiu Xu, Jinqiu Li, Junqi Ma, Ayshamgul Hasim   +3 more
wiley   +1 more source

Data Mining for the Characterization of a Paper Prototype Obtained with Bacterial Cellulose Derived from Banana and Pineapple By-Products

Applied Sciences
The primary objective of this research is to evaluate the feasibility of two of the most prevalent agricultural residues in Ecuador, banana peels and pineapple peels, as a carbon source in the culture medium of Komagataeibacter hansenii for the ...
Juan Diego Valenzuela-Cobos, Simón Pérez-Martínez, Manuel Fiallos-Cárdenas, Fabricio Guevara-Viejó   +3 more
doaj   +1 more source

Mathematical modelling of fibre-enhanced perfusion inside\ud a tissue-engineering bioreactor [PDF]

, 2009
We develop a simple mathematical model for forced flow of culture medium through a porous scaffold in a tissue- engineering bioreactor. Porous-walled hollow fibres penetrate the scaffold and act as additional sources of culture medium.
Bailey, Clare, Booth, Richard, Cartmell, Sarah, Chaudhuri, Julian B, Chini, Louise Parsons, Cummings, Linda J, Dyson, Rosemary, Ellis, Marianne J, Kuiper, Nicola J, Mawasse, Lina, Michael, Vipin, Naire, Shailesh, Payvandi, Sevil, Rong, Zimei, Waters, Sarah L, Whittaker, Robert J, Woollard, Hannah   +16 more
core  

Production of selenium-enriched yeast (Kluyveromyces marxianus) biomass in a whey-based culture medium [PDF]

, 2018
Two important aspects of agriculture intensification are the reduction in the concentration of specific soil minerals that affects livestock production and the increase of agricultural by-products, which produce environmental pollution.
Calafat, Mario Jose, Gigli, Isabel, Gurdo, Nicolás, Noseda, Diego Gabriel   +3 more
core   +1 more source

Integrative systems‐level analysis reveals a contextual crosstalk between hypoxia and global metabolism in human breast tumors

Molecular Oncology, Volume 19, Issue 6, Page 1725-1736, June 2025.
Breast tumor samples scored for metabolic deregulation (M1 to M3) were given a hypoxia score (HS). The highest HS occurred in patients with strongest metabolic deregulation (M3), supporting tumor aggressiveness. HS correlated with the highest number of metabolic pathways in M1. This suggests hypoxia to be an early event in metabolic deregulation.
Raefa Abou Khouzam, Salem Chouaib, Mohammad Askandar Iqbal   +2 more
wiley   +1 more source

Mathematical Modeling of Perifusion Cell Culture Experiments on GnRH Signaling [PDF]

arXiv, 2015
The effects of pulsatile GnRH stimulation on anterior pituitary cells are studied using perifusion cell cultures, where constantly moving medium over the immo- bilized cells allows intermittent GnRH delivery. The LH content of the outgoing medium serves as a readout of the GnRH signaling pathway activation in the cells.
arxiv  

Optimization of BY-2 cell suspension culture medium for the production of a human antibody using a combination of fractional factorial designs and the response surface method.

Plant Biotechnology Journal, 2013
We have developed a strategy for the optimization of plant cell suspension culture media using a combination of fractional factorial designs (FFDs) and response surface methodology (RSM).
N. Vasilev, U. Grömping, A. Lipperts, N. Raven, R. Fischer, S. Schillberg   +5 more
semanticscholar   +1 more source

The impact of organisational culture on WiMax adoption by Saudi SMEs [PDF]

, 2011
Although there is some research that examines Worldwide Inter-operability for Microwave Access (WiMax) adoption, the role of the organisational culture by Small and Medium Enterprises (SMEs) has not been studied in the context of the Kingdom of Saudi ...
Abousaber, I, Papazafeiropoulou, A
core