Results 21 to 30 of about 8,999,056 (423)

Comprehensive evaluation of methods for small extracellular vesicles separation from human plasma, urine and cell culture medium

Journal of Extracellular Vesicles, 2020
One of the challenges that restricts the evolving extracellular vesicle (EV) research field is the lack of a consensus method for EV separation. This may also explain the diversity of the experimental results, as co‐separated soluble proteins and ...
L. Dong, Richard C. Zieren, Kengo Horie, Chi-Ju Kim, E. Mallick, Yuezhou Jing, Mingxiao Feng, M. Kuczler, J. Green, Sarah R Amend, K. Witwer, T. D. de Reijke, Yoon‐Kyoung Cho, K. Pienta, W. Xue   +14 more
semanticscholar   +1 more source

A novel three step protocol to isolate extracellular vesicles from plasma or cell culture medium with both high yield and purity

Journal of Extracellular Vesicles, 2020
Extracellular vesicles (EV) are membrane encapsulated nanoparticles that can function in intercellular communication, and their presence in biofluids can be indicative for (patho)physiological conditions.
Xiaogang Zhang, Ellen G. F. Borg, A. Liaci, H. Vos, W. Stoorvogel   +4 more
semanticscholar   +1 more source

Rational design of an acidic erythritol (ACER) medium for the enhanced isolation of the environmental pathogen Burkholderia pseudomallei from soil samples

Frontiers in Microbiology, 2023
The soil bacterium Burkholderia pseudomallei causes melioidosis, a potentially fatal and greatly underdiagnosed tropical disease. Detection of B. pseudomallei in the environment is important to trace the source of infections, define risk areas for ...
Karoline Assig, Sabine Lichtenegger, Linh N. H. Bui, Bettina Mosbacher, Anh T. N. Vu, Daniel Erhart, Trung T. Trinh, Ivo Steinmetz   +7 more
doaj   +1 more source

Osteostatin potentiates the bioactivity of mesoporous glass scaffolds containing Zn2+ ions in human mesenchymal stem cell cultures [PDF]

Acta Biomaterialia. 89 (2019) 359-371, 2021
There is an urgent need of biosynthetic bone grafts with enhanced osteogenic capacity. In this study, we describe the design of hierarchical meso-macroporous 3D-scaffolds based on mesoporous bioactive glasses (MBGs), enriched with the peptide osteostatin and Zn2+ ions, and their osteogenic effect on human mesenchymal stem cells (hMSCs) as a preclinical
arxiv   +1 more source

Culture Strategies for Isolation of Fastidious Leptospira Serovar Hardjo and Molecular Differentiation of Genotypes Hardjobovis and Hardjoprajitno

Frontiers in Microbiology, 2017
The Leptospira serovar Hedjo belongs to the serogroup sejroe and this serovar is the most prevalent in bovine herds worldwide. The sejroe serogroup is the most frequently detected by serology in Brazilian cattle herds suggesting that it is due serovar ...
Roberta T. Chideroli, Daniela D. Gonçalves, Suelen A. Suphoronski, Alice F. Alfieri, Amauri A. Alfieri, Admilton G. de Oliveira, Julio C. de Freitas, Ulisses de Padua Pereira   +7 more
doaj   +1 more source

In Vitro Propagation of Pyracantha angustifolia (Franch.) C.K. Schneid.

Horticulturae, 2022
Narrow-leaf firethorn or pyracantha (Pyracantha angustifolia (Franch.) C.K. Schneid.), from the family Rosaceae, is a species of large and thorny evergreen shrub.
Behzad Kaviani, Bahareh Deltalab, Dariusz Kulus, Alicja Tymoszuk, Hamideh Bagheri, Taha Azarinejad   +5 more
doaj   +1 more source

A new cell primo-culture method for freshwater benthic diatom communities [PDF]

, 2009
A new cell primo-culture method was developed for the benthic diatom community isolated from biofilm sampled in rivers. The approach comprised three steps: (1) scraping biofilm from river pebbles, (2) diatom isolation from biofilm, and (3) diatom ...
A Berard, BA Whitton, C Butterwick, C Nalewajko, E Navarro, E Rott, E. Pinelli, F Kasai, F Peres, F-J DeNoyelles, F. Delmas, FE Round, H Dam Van, H Guasch, H Guasch, H Guasch, H Lange-Bertalot, HG Peterson, HW Nichols, J Prygiel, J. Silvestre, JX Tang, K Gustavson, KJ Nelson, M. Coste, P McCormick, R Casotti, RJ Stevenson, RRL Guillard, S Sabater, SA Badr, T Hayakawa, T. Debenest, U Dorigo, U Schreiber   +34 more
core   +3 more sources

Cell Types Used for Cultured Meat Production and the Importance of Myokines

Foods, 2021
The world’s population continues to increase, meaning we require more consistent protein supply to meet demand. Despite the availability of plant-based protein alternatives, animal meat remains a popular, high-quality protein source.
Sibhghatulla Shaikh, Eunju Lee, Khurshid Ahmad, Syed-Sayeed Ahmad, Heejin Chun, Jeongho Lim, Yongho Lee, Inho Choi   +7 more
doaj   +1 more source

Improving the metabolic fidelity of cancer models with a physiological cell culture medium

Science Advances, 2019
Plasmax, a physiological cell culture medium, prevents metabolic artifacts imposed on cancer cells by commonly used media. Currently available cell culture media may not reproduce the in vivo metabolic environment of tumors.
J. Vande Voorde, Tobias Ackermann, Nadja Pfetzer, D. Sumpton, G. Mackay, G. Kalna, C. Nixon, K. Blyth, E. Gottlieb, S. Tardito   +9 more
semanticscholar   +1 more source

Cultivation of Autochthonous Microalgae for Biomass Feedstock: Growth Curves and Biomass Characterization for Their Use in Biorefinery Products

Energies, 2021
In this work, the biomass productivity for biorefinery products and growth curves of three autochthonous microalgae collected in different reservoirs (“Scenedesmus sp.” (SSP), mixture of Scenedesmus sp., Chlorella minutissima, Chlorellas sp.
Juan Félix González, Teresa Belén Cuello, Antonio José Calderón, Manuel Calderón, Jerónimo González, Diego Carmona   +5 more
doaj   +1 more source