A functional motor unit in the culture dish : co-culture of spinal cord explants and muscle cells [PDF]
, 2012 Human primary muscle cells cultured aneurally in monolayer rarely contract spontaneously because, in the absence of a nerve component, cell differentiation is limited and motor neuron stimulation is missing(1). These limitations hamper the in vitro study
Arnold, Anne-Sophie +2 more
core +1 more source
Magnetofection potentiates gene delivery to cultured endothelial cells [PDF]
, 2003 Modification of cellular functions by overexpression of genes is increasingly practised for research of signalling pathways, but restricted by limitations of low efficiency. We investigated whether the novel technique of magnetofection (MF) could enhance
Gloe, Torsten +4 more
core +1 more source
Tolerance to mutations in the foot-and-mouth disease virus integrin-binding RGD region is different in cultured cells and in vivo and depends on the capsid sequence context. [PDF]
, 2008 Engineered RNAs carrying substitutions in the integrin receptor-binding Arg-Gly-Asp (RGD) region of foot-and-mouth disease virus (FMDV) were constructed (aa 141-147 of VP1 capsid protein) and their infectivity was assayed in cultured cells and suckling
Baranowski, Eric +4 more
core +3 more sources
Hybrid cell lines producing monoclonal antibodies directed against Treponema [PDF]
, 1985 Continuous hybrid cell lines for producing monoclonal antibodies directed against antigens of Treponema pallidum have been developed. The hybrid cell lines were established by fusing differentiated lymphoid cells primed with antigens of Treponema ...
Kettman, John R., Norgard, Michael V.
core +1 more source
Annals of Hepatology, 2007 HepG2 human hepatoma cells were incubated for 24 or 48 h with various concentrations of YHK solution. After 24 h incubation, cell proliferation and cytotoxicity were determined by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl ...
Francesco Marotta +5 more
doaj +1 more source
Journal of Animal Science and Technology, 2023 To improve culture efficiency of Hanwoo myosatellite cells, these cells were cultured at different temperatures. Hanwoo myosatellite cells were compared with C2C12 cells to observe proliferation and differentiation at culture temperatures of 37°C and 39 ...
Sehyuk Oh +10 more
doaj +1 more source
Platelet lysate as a serum substitute for 2D static and 3D perfusion culture of stromal vascular fraction cells from human adipose tissue [PDF]
, 2009 Fetal bovine serum (FBS) and fibroblast growth factor (FGF)-2 are key supplements for the culture of stromal vascular fraction (SVF) cells from adipose tissue, both for typical monolayer (2D) expansion and for streamlined generation of osteogenic ...
Alini, Mauro +8 more
core +1 more source
Fibrin gel as a scaffold for skin substitute – production and clinical experience
Acta Clinica Croatica, 2016 The purpose of this study was to create a fibrin-based human skin substitute in vitro with epidermal and dermal component and to assess its healing potential in deep partial and full thickness burns.
Antun Kljenak +9 more
doaj +1 more source
Long-term cultures of murine fetal liver retain very early B lymphoid phenotype. [PDF]
, 1984 Long-term cultures of murine fetal liver have been successfully established using a modification of our in vitro bone marrow culture system (14, 15).
Denis, KA +3 more
core
Organoids in pediatric cancer research
FEBS Letters, EarlyView.Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley +1 more source