Detection and characterization of Sp1 binding activity in human chondrocytes and its alterations during chondrocyte dedifferentiation. [PDF]
We have detected DNA binding activity for a synthetic oligonucleotide containing an Sp1 consensus sequence in nuclear extracts from human chondrocytes. Changes in the levels of Sp1 oligonucleotide binding activity were examined in nuclear extracts from ...
Dharmavaram, Rita M.+3 more
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Demonstration of astrocytes in cultured amniotic fluid cells of three cases with neural-tube defect [PDF]
We have investigated the origin of rapidly adhering (RA) cells in three cases of neural tube defects (two anencephali, one encephalocele). We were able to demonstrate the presence of glial fibrillary acidic (GFA) protein in variable percentages (4–80 ...
AD Smith+18 more
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Genomics, proteomics, vaccinology, transgenics, stem cell—advances in all these areas critically stack on the shoulders of tissue culture, our ability to cultivate an organism's living cells in plastic dishes. Nutritional trial and error for decades of painstaking cell gardening laid the groundwork for the several thousand human primary cell explants ...
openaire +3 more sources
Aims: This in vitro study aimed to determine the mode of cell death of co-cultured dental mesenchymal stem cells following exposure to different irrigating solutions.
Eshagh Ali Saberi+3 more
doaj +1 more source
Microelectrode arrays of diamond-insulated graphitic channels for real time detection of exocytotic events from cultured chromaffin cells and slices of adrenal glands [PDF]
A microstructured graphitic 4x4 multielectrode array was embedded in a single crystal diamond substrate (4x4 {uG-SCD MEA) for real-time monitoring of exocytotic events from cultured chromaffin cells and adrenal slices.
Battiato, A.+7 more
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Primary osteoblast-like cells from patients with end-stage kidney disease reflect gene expression, proliferation, and mineralization characteristics ex vivo. [PDF]
Osteocytes regulate bone turnover and mineralization in chronic kidney disease. As osteocytes are derived from osteoblasts, alterations in osteoblast function may regulate osteoblast maturation, osteocytic transition, bone turnover, and skeletal ...
Bowen, Richard E+6 more
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Fibrin gel as a scaffold for skin substitute – production and clinical experience
The purpose of this study was to create a fibrin-based human skin substitute in vitro with epidermal and dermal component and to assess its healing potential in deep partial and full thickness burns.
Antun Kljenak+9 more
doaj +1 more source
A method for isolating and culturing placental cells from failed early equine pregnancies [PDF]
Early pregnancy loss occurs in 6–10% of equine pregnancies making it the main cause of reproductive wastage. Despite this, reasons for the losses are known in only 16% of cases.
A.J. McGladdery+31 more
core +1 more source
Long-term cultures of murine fetal liver retain very early B lymphoid phenotype. [PDF]
Long-term cultures of murine fetal liver have been successfully established using a modification of our in vitro bone marrow culture system (14, 15).
Denis, KA+3 more
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