Lethal impacts of cigarette smoke in cultured tobacco cells
Tobacco Induced Diseases, 2011 Background In order to understand and generalize the toxic mechanism of cigarette smoke in living cells, comparison of the data between animal systems and other biological system such as microbial and plant systems is highly beneficial.
Masaru Yukihiro +2 more
doaj +1 more source
Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer
FEBS Letters, EarlyView.Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani +4 more
wiley +1 more source
Impaired bone marrow homing of cytokine-activated CD34+ cells in the NOD/SCID model [PDF]
, 2004 The reduced engraftment potential of hematopoietic stem/progenitor cells (HSPCs) after exposure to cytokines may be related to the impaired homing ability of actively cycling cells. We tested this hypothesis by quantifying the short-term horning of human
Ahmed, F +8 more
core
Hedgehog Signalling in Androgen Independent Prostate Cancer [PDF]
, 2008 Objectives: Androgen-deprivation therapy effectively shrinks hormone-naïve prostate cancer, both in the prostate and at sites of distant metastasis.
Bisson, Isabelle +7 more
core +1 more source
Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing
FEBS Letters, EarlyView.Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley +1 more source
Three dimensional neuronal cell cultures more accurately model voltage gated calcium channel functionality in freshly dissected nerve tissue. [PDF]
PLoS ONE, 2012 It has been demonstrated that neuronal cells cultured on traditional flat surfaces may exhibit exaggerated voltage gated calcium channel (VGCC) functionality.
Yinzhi Lai, Ke Cheng, William Kisaalita
doaj +1 more source
Differing calcification processes in cultured vascular smooth muscle cells and osteoblasts [PDF]
, 2019 © 2019 Published by Elsevier Inc.Arterial medial calcification (AMC) is the deposition of calcium phosphate mineral, often as hydroxyapatite, inthe medial layer of the arteries.
Arnett, Timothy R +6 more
core +5 more sources
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source
Induction of αCaMKII Expression by KCl in Cultured Primary Cerebellar Granule Neurons [PDF]
Caspian Journal of Neurological SciencesBackground: The cerebellum specializes in functions, such as motor learning. One of the abundant types of neuronal cells present in the cerebellum is the cerebellar granule (CG) cell.
Ramya R Prabhu
doaj
Distinct cell stress responses induced by ATP restriction in quiescent human fibroblasts
Frontiers in Genetics, 2016 Quiescence is the prevailing state of many cell types under homeostatic conditions. Yet, surprisingly little is known about how quiescent cells respond to energetic and metabolic challenges.
Nirupama Yalamanchili +5 more
doaj +1 more source