Results 71 to 80 of about 12,036,045 (356)

From omics to AI—mapping the pathogenic pathways in type 2 diabetes

FEBS Letters, EarlyView.
Integrating multi‐omics data with AI‐based modelling (unsupervised and supervised machine learning) identify optimal patient clusters, informing AI‐driven accurate risk stratification. Digital twins simulate individual trajectories in real time, guiding precision medicine by matching patients to targeted therapies.
Siobhán O'Sullivan, Lu Qi, Pierre Zalloua   +2 more
wiley   +1 more source

Effects of light-curing time on the cytotoxicity of a restorative composite resin on odontoblast-like cells

Journal of Applied Oral Science, 2010
This in vitro study evaluated the cytotoxicity of an experimental restorative composite resin subjected to different light-curing regimens. METHODS: Forty round-shaped specimens were prepared and randomly assigned to four experimental groups (n=10), as ...
Andreza Maria Fábio Aranha, Elisa Maria Aparecida Giro, Josimeri Hebling, Fernanda Campos Rosetti Lessa, Carlos Alberto de Souza Costa   +4 more
doaj   +1 more source

Mycobacterium tuberculosis sulfurtransferase SseA is activated by its neighboring gene product Rv3284

FEBS Letters, EarlyView.
Tuberculosis remains a global health challenge and new therapeutic targets are required. Here, we characterized SseA, a sulfurtransferase from Mycobacterium tuberculosis involved in macrophage infection, and its interaction with the newly identified protein SufEMtb that activates SseA enzymatic activity.
Giulia Di Napoli, Alex Fissore, Edoardo Salladini, Eleonora Raccuia, Simonetta Oliaro‐Bosso, Alessia Ruggiero, Rita Berisio, Milagros Medina, Adrian Velazquez‐Campoy, Salvatore Adinolfi, Mauro Marengo   +10 more
wiley   +1 more source

Serum-Free Cryopreservation of Five Mammalian Cell Lines in Either a Pelleted or Suspended State

Biological Procedures Online, 2004
Herein we have explored two practical aspects of cryopreserving cultured mammalian cells during routine laboratory maintenance. First, we have examined the possibility of using a serum-free, hence more affordable, cryopreservative.
Corsini Joe, Hacker Christy, Bare Charles   +2 more
doaj   +1 more source

Meganuclease-mediated Inhibition of HSV1 Infection in Cultured Cells.

Molecular Therapy, 2011
Herpes simplex virus type 1 (HSV1) is a major health problem. As for most viral diseases, current antiviral treatments are based on the inhibition of viral replication once it has already started.
S. Grosse, N. Huot, C. Mahiet, S. Arnould, S. Barradeau, D. L. Clerre, I. Chion-Sotinel, Cécile Jacqmarcq, B. Chapellier, A. Ergani, C. Desseaux, F. Cédrone, E. Conseiller, F. Pâques, M. Labetoulle, J. Smith   +15 more
semanticscholar   +1 more source

Archaeal protein containing domain of unknown function 2193 undergoes oligomeric reconfiguration upon iron–sulfur cluster binding

FEBS Letters, EarlyView.
This work presents the characterization of MvoDUF2193, a Methanococcus voltae (Mvo) protein from the domain of unknown function (DUF) 2193 family. We demonstrate that MvoDUF2193 binds a single [4Fe–4S] cluster per subunit and that cluster occupancy regulates the transition from an apo tetramer to a [4Fe–4S] monomeric form. This structural transition is
Emily M. Dieter, James Larson, Monika Tokmina‐Lukaszewska, Jin Xiong, Jared Green, Yisong Guo, William E. Broderick, Brian Bothner, Joan B. Broderick   +8 more
wiley   +1 more source

Differential Response to Cisplatin between Co-cultured Cells and Pure Cultured Cells Based on Single-cell RNA Sequencing of Three-dimensional-cultured Breast Cancer Cells

Frontiers in Bioscience-Landmark
Objective: The current study aimed to develop an experimental approach for the direct co-culture of three-dimensional breast cancer cells using single-cell RNA sequencing (scRNA-seq ...
Shuqing Yang, Peixian Chen, Xiaofan Mao, KaiRong Lin, Wei Li, Tiancheng He, Huiqi Huang, AiGuo Wu, Wei Luo, Guolin Ye, Guangyu Yao, Dan Zhou   +11 more
doaj   +1 more source

Enhanced extracellular production of trans-resveratrol in Vitis vinifera suspension cultured cells by using cyclodextrins and methyljasmonate

Plant Cell Reports, 2011
In this work, the effect of different inducing factors on trans-resveratrol extracellular production in Monastrell grapevine suspension cultured cells is evaluated. A detailed analysis provides the optimal concentrations of cyclodextrins, methyljasmonate
S. Belchí-Navarro, L. Almagro, D. Lijavetzky, R. Bru, M. Pedreño   +4 more
semanticscholar   +1 more source

ERBIN limits epithelial cell plasticity via suppression of TGF‐β signaling

FEBS Letters, EarlyView.
In breast and lung cancer patients, low ERBIN expression correlates with poor clinical outcomes. Here, we show that ERBIN inhibits TGF‐β‐induced epithelial‐to‐mesenchymal transition in NMuMG breast and A549 lung adenocarcinoma cell lines. ERBIN suppresses TGF‐β/SMAD signaling and reduces TGF‐β‐induced ERK phosphorylation.
Chao Li, Gerard van der Zon, Peter ten Dijke, Tong Shen   +3 more
wiley   +1 more source

Label-free classification of cultured cells through diffraction imaging

Biomedical Optics Express, 2011
Automated classification of biological cells according to their 3D morphology is highly desired in a flow cytometer setting. We have investigated this possibility experimentally and numerically using a diffraction imaging approach.
K. Dong, Yuanming Feng, K. Jacobs, Jun Q. Lu, R. S. Brock, Li V. Yang, F. E. Bertrand, M. Farwell, Xin-Hua Hu   +8 more
semanticscholar   +1 more source