Purification and Some Properties of Cyclomaltodextrin Glucanotransferase fromBacillus circulans
Agricultural and Biological Chemistry, 1988 Cyclomaltodextrin glucanotransferase was purified from B. circulans C31 through two successive steps of starch and Biogel column chromatography. The enzyme was purified up to 90-fold with a 30% yield. Its molecular weight was around 103,000.
Piamsook PONGSAWASDI, Mitsuo YAGISAWA
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Potential cyclodextrin glycosyltransferase producer from locally isolated bacteria [PDF]
, 2016 Cyclodextrin glycosyltransferase (CGTase) is one of the most important groups of microbial amylolytic enzymes that have been used for degradation of starch to yield cyclodextrin (CD) via cyclization reaction.
Abd-Aziz, Suraini +4 more
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Studies on cyclomaltodextrin glucanotransferase and Coupling sugar.
Journal of the Japanese Society of Starch Science, 1987 The topics from the results of the studies conducted by the author and collaborators during the two decades are reviewed. The studies are mainly related to cyclodextrin glycosyltransferase (CGT-ase) and include the following subjects.1) Screening of glucosyltransferase from soil bacteria.2) B. megaterium CGT-ase purification and action on starch and co-
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Synthesis and Structure Analysis of Glucosylpsicose Produced by Cyclomaltodextrin Glucanotransferase
Journal of Applied Glycoscience, 2008 Glucosylpsicose was synthesized by transglycosylation with α-cyclodextrin and D-psicose using cyclomaltodextrin glucanotransferase (EC 2.4.1.19; CGTase). The structure of glucosylpsicose was analyzed by HPLC to determine sugar composition and molecular mass, by methylation analysis using GC-MS and by α- and β-glucosidase treatments to determine linkage,
Hisaka Oshima +3 more
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Extremophilic Microorganisms as a Source of Emerging Enzymes for the Food Industry: A Review
Food Science &Nutrition, Volume 13, Issue 1, January 2025.Enzymes such as amylases, lipases, proteases, xylanases, laccases, pullulanase, chitinases, pectinases, esterases, isomerases, and dehydrogenases could be derived from extremophilic organisms like thermophiles, psychrophiles, acidophiles, alkaliphiles, and halophiles. These enzymes or extremozymes are applicable in the production of safe foods (such as
Tolulope Joshua Ashaolu +4 more
wiley +1 more source
Cyclodextrin Formation by the Thermostable α-Amylase of Thermoanaerobacterium thermosulfurigenes EM1 and Reclassification of the Enzyme as a Cyclodextrin Glycosyltransferase [PDF]
, 1995 Extensive characterization of the thermostable α-amylase of Clostridium thermosulfurogenes EM1, recently reclassified as Thermoanaerobacterium thermosulfurigenes, clearly demonstrated that the enzyme is a cyclodextrin glycosyltransferase (CGTase ...
Bahl, Hubert +6 more
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An alkaliphilic cyclodextrin glycosyltransferase from a new Bacillus agaradhaerens WN-I strain isolated from an Egyptian soda lake: Purification and properties [PDF]
, 2013 Alkaliphilic bacteria were isolated from soil and water samples obtained from Egyptian soda lakes in the Wadi Natrun area. Screening for cyclodextrin glycosyltransferase- producing alkaliphilic bacteria resulted in the isolation of 15 positive strains ...
Al-Salamah, AA, Bahi, H, Ibrahim, ASS
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Recombinant cyclodextran glucanotransferase mutants [PDF]
, 1998 A recombinant cyclodextrin glucanotransferase mutant is provided, which mutant produces lowered amounts of β-cyclodextrins and γ-cyclodextrins compared to wild-type cyclodextrin ...
Chang, Hai-Yin, Nikolov, Zivko L.
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Isolation, Purification and Biochemical Characterization of CGTase from Bacillus halodurans [PDF]
, 2012 A novel Cyclomaltodextrin glucanotransferase (CGTase) producer, Bacillus halodurans was isolated from soil obtained from sugarcane fi elds. CGTase was produced in bulk through submerged batch fermentation in Horikoshi’s Media II.
Akshata M. Byadgi +5 more
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Improvement of 2-O-α-D-Glucopyranosyl-L-Ascorbic Acid Biosynthesis Using Ultrasonic Radiation [PDF]
, 2015 Purpose: To improve 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G) production using ultrasonic radiation (UR) treatment.Methods: The production of AA-2G using UR or ultrasonic radiation with shaking (URS) at 150 rpm, at varying power (100 − 500 W ...
Bashari, MO +4 more
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