Immunoaffinity Purification and Characterization of Cyclodextrin Glycosyltransferase from Bacillus circulans A11 [PDF]
The purified IgG fraction of anti-CGTase was coupled to CNBr-activated Sepharose 4B and used as immunoaffinity gel to purify CGTase from Bacillus circulans A11. The enzyme was successfully purified to approximately 155 folds with a 45% yield and specific
Anchalee Tongsima +6 more
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Safety evaluation of the food enzyme dextranase from Collariella gracilis strain ATCC-16153 [PDF]
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Andryszkiewicz, Magdalena +20 more
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Optimization of process parameters of cyclodextrin glycosyltransferase isolated from novel mutated Bacillus sp. TPR71HNA6 by Taguchi orthogonal array method [PDF]
Cyclodextrin glycosyl transferase CGTase) production by Bacillus SP. TPR71HNA6 under shake flask culture was optimized following Taguchi orthogonal array (OA) design of experiment. An OA layout of L16 was constructed with five most influensive ...
Prabhakar, Tadimalla +3 more
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Produção da enzima ciclodextrina glicosil transferase de Bacillus firmus cepa 37 em Escherichia coli [PDF]
Orientador: Prof. Dr. Marco Aurélio Schuler de OliveiraDissertação (mestrado em Bioquímica)-Universidade Estadual de Maringá, 2023RESUMO: O amido é um carboidrato polimérico formado por muitas unidades de glicose unidas covalentemente, sendo o principal ...
Junqueira, Larissa Aparecida Ricardini
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Polysaccharide-synthesizing Glycosyltransferases and Carbohydrate Binding Modules: the case of Starch Synthase III [PDF]
Glycosyltransferases (GTs) are a ubiquitous group of enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds.
Busi, María Victoria +2 more
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Purification and characterization of a thermostable cyclodextrin glycosyltransferase from Thermoanaerobacter sp. P4 [PDF]
Cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) from a thermophilic anaerobic bacterium, Thermoanaerobacter sp. P4, was purified by ammonium sulfate precipitation followed by α-cyclodextrin epoxy activated-sepharose 6B column chromatography ...
Avci, Ayse, Dönmez, Sedat
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A quick and reliable assay was developed to measure the cyclization activity of β-cyclodextrin-forming cyclomaltodextrin glucanotransferase. Beta-cyclodextrin formed in the reaction was measured directly in situ through as a complex with phenolphthalein. Using this assay, it was shown that the crude enzyme from an alkalophilic Bacillus sp. (ATCC 21783)
Takahiro KANEKO +3 more
openaire +2 more sources
Usefulness of Organic Acid Produced by Exiguobacterium sp. 12/1 on Neutralization of Alkaline Wastewater [PDF]
The aim of this study was to investigate the role of organic acids produced by Exiguobacterium sp. strain 12/1 (DSM 21148) in neutralization of alkaline wastewater emanated from beverage industry.
Bisht, Gopal +4 more
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Molecular identification of a cyclodextrin glycosyltransferase-producinmicroorganism and phylogenetic assessment of enzymatic activities [PDF]
Cyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries.
Caminata Landriel, Soledad +5 more
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This paper presents results on the effect of fermentation variables on CGTase production from Bacillus stearothermophilus HR1. Initial screening of temperature, pH and agitation speed was determined using a 2-level full factorial design.
Abd. Rahman, Roshanida +5 more
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