Results 161 to 170 of about 2,862 (193)

Primary Establishment of LAMP Method for Cyprinid Herpesvirus 2 Detection

Applied Mechanics and Materials, 2014
A new loop-mediated isothermal amplification (LAMP) method for the detection of Cyprinid herpesvirus 2 (CyHV-2) was developed. Following the synthesis in vitro of a 413 bp DNA fragment of CyHV2 DNA-dependent DNA polymerase gene by overlap extension PCR using 11 overlapping oligo primers designed according to the gensome sequences information of CyHV2 ...
Ze Xiao Yang, Gui Li Li, Yin Wang, Xue Ping Yao, Kai Yu Wang, Shan Zhen Peng, Bo Wang, Hou Xun Ya, Ran Yang Ren   +8 more
openaire   +1 more source

Generation and application of a monoclonal antibody specific for the ORF121 of cyprinid herpesvirus 2

Journal of Fish Diseases, 2021
AbstractCyprinid herpesvirus 2 (CyHV‐2) is a viral pathogen worldwide and causing high mortality on goldfish and silver crucian carp (Carassius auratus gibelio). In order to establish a stable and sensitive immunological diagnostic approach, the recombinant ORF121 protein encoded by the CyHV‐2 ORF121 gene, was selected as a capture antigen to identify ...
Wa Gao, Lupin Zhao, Yihua Zheng, Kaixuan Wu, Feiyang Xu, Hao Wang, Liqun Lu, Yousheng Jiang   +7 more
openaire   +2 more sources

Reactivation of cyprinid herpesvirus 2 (CyHV-2) in asymptomatic surviving goldfish Carassius auratus (L.) under immunosuppression

Fish & Shellfish Immunology, 2020
Cyprinid herpesvirus 2 (CyHV-2) is a highly contagious pathogen of goldfish (Carassius auratus) and Prussian carp (Carassius auratus gibelio) causing herpesviral hematopoietic necrosis. Our previous study revealed that CyHV-2 can persistently infect the kidney and spleen of goldfish that recovered from a primary infection.
Chang, Wei, Taichi, Kakazu, Qiu Yuan, Chuah, Mikio, Tanaka, Goshi, Kato, Motohiko, Sano   +5 more
openaire   +2 more sources

Goldfish Hematopoietic Necrosis Herpesvirus (Cyprinid Herpesvirus 2) in the USA: Molecular Confirmation of Isolates from Diseased Fish

Journal of Aquatic Animal Health, 2006
Abstract Moribund goldfish Carassius auratus from private collections and breeding facilities were tested for hematopoietic necrosis herpesvirus 2 (also known as cyprinid herpesvirus 2 (CyHV-2)) by degenerate polymerase chain reaction (PCR) of the viral polymerase gene followed by sequencing. The degenerate PCR method produced a fragment
A. E. Goodwin, L. Khoo, S. E. LaPatra, C. Bonar, D. W. Key, M. Garner, M. V. Lee, L. Hanson   +7 more
openaire   +1 more source

In vitro characteristics of cyprinid herpesvirus 2: effect of kidney extract supplementation on growth

Diseases of Aquatic Organisms, 2015
Herpesviral hematopoietic necrosis caused by goldfish hematopoietic necrosis virus (now identified as cyprinid herpesvirus 2, CyHV-2) has contributed to economic losses in goldfish Carassius auratus culture and is becoming a major obstacle in Prussian carp C. gibelio aquaculture in China.
Tomoya, Shibata, Azusa, Nanjo, Masato, Saito, Keisuke, Yoshii, Takafumi, Ito, Teruyuki, Nakanishi, Takashi, Sakamoto, Motohiko, Sano   +7 more
openaire   +2 more sources

Development of a Polymerase Chain Reaction Assay to Detect Cyprinid Herpesvirus 2 in Goldfish

Journal of Aquatic Animal Health, 2009
Abstract Cyprinid herpesvirus 2 (CyHV2) has been associated with epidemic mortality among cultured populations of goldfish Carassius auratus. As the principal target tissues are hematopoietic cells in the kidney and spleen, the disease is designated herpesviral hematopoietic necrosis (HVHN).
Thomas B, Waltzek, Tomofumi, Kurobe, Andrew E, Goodwin, Ronald P, Hedrick   +3 more
openaire   +2 more sources

First detection of Cyprinid Herpesvirus 2 (CyHV‐2) in goldfish (Carassius auratus) in France

Journal of Fish Diseases, 2015
AbstractMassive mortalities of Carassius auratus (L.) occurred in a farm in France during summer 2014. Fish presented anorexia, loss of scales and large amounts of mucus on the gills. Necrosis of the distal tip of the filament and the lamellae, combined with fusion of the lamellae, was observed, as well as necrosis in the hematopoietic organs and in ...
P-M, Boitard, M, Baud, S, Labrut, C, de Boisséson, M, Jamin, L, Bigarré   +5 more
openaire   +2 more sources

Cyprinid herpesvirus 2 (CyHV-2) ORF67 inhibits IFN expression by competitively obstructing STING phosphorylation

Fish & Shellfish Immunology
Cyprinid herpesvirus 2 (CyHV-2) caused hematopoietic necrosis in crucian carp (Carassius auratus) has resulted in huge economic losses to the carp aquaculture. Interferon (IFN) response serves as a crucial line of defense for fish against CyHV-2 infection; however, CyHV-2 frequently overcomes this defense, resulting in damage and even substantial ...
Bao-Jie Cui, Can Zhang, Chu-Jing Zhou, Zhuo-Cong Li, Xiao Xu, Yang-Yang Wang, Dan-Dan Chen, Li Zhou, Long-Feng Lu, Shun Li   +9 more
openaire   +2 more sources

Incursions of Cyprinid herpesvirus 2 in goldfish populations in Australia despite quarantine practices

Aquaculture, 2014
The international trade in ornamental fish is considered a major factor for the transboundary spread of aquatic pathogens that can affect both wild and farmed fish populations. Nearly 18 million ornamental fish were imported into Australia in 2007, including approximately 3.9 million goldfish.
Joy A. Becker, Alison Tweedie, Anneke Rimmer, Matthew Landos, Mark Lintermans, Richard J. Whittington   +5 more
openaire   +1 more source

Combined transcriptomic/proteomic analysis of crucian carp Carassius auratus gibelio in cyprinid herpesvirus 2 infection

Fish & Shellfish Immunology, 2018
Cyprinid herpesvirus 2 (CyHV-2) is a pathogen of herpesviral hematopoietic necrosis disease of crucian carp. Our study aimed to investigate the molecular mechanisms and immune response at the mRNA and protein levels in head kidney during CyHV-2 infection.
Min Liu, Ting Wu, Shuang Li, Panpan Wei, Yuye Yan, Wei Gu, Wen Wang, Qingguo Meng   +7 more
openaire   +2 more sources