Lysosomal cysteine endopeptidases mediate interleukin 1-stimulated cartilage proteoglycan degradation [PDF]
Biochemical Journal, 1992 The peptidyl diazomethane inactivator of cysteine endopeptidases, benzyloxycarbonyl-Tyr-Ala-CHN2, was tested as an inhibitor of interleukin 1 alpha-stimulated release of proteoglycan from bovine nasal septum cartilage explants. Like the previously tested epoxidyl peptide proinhibitor trans-epoxysuccinyl-leucylamido-(3-methyl)butane ethyl ester, it ...
David J. Buttle, Jeremy Saklatvala
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Expression and Secretion of Barley Cysteine Endopeptidase B and Cellobiohydrolase I in Trichoderma reesei [PDF]
Applied and Environmental Microbiology, 1997 Localization of expression and secretion of a heterologous barley cysteine endopeptidase (EPB) and the homologous main cellobiohydrolase I (CBHI) in a Trichoderma reesei transformant expressing both proteins were studied. The transformant was grown on solid medium with Avicel cellulose and lactose to induce the cbh1 promoter for the synthesis of the ...
Marko Nykänen +4 more
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Two New Cysteine Endopeptidases Obtained from the Latex of Araujia hortorum Fruits [PDF]
Journal of Protein Chemistry, 2001 Two new endopeptidases were purified to homogeneity from the latex of Araujia hortorum fruits by a simple purification procedure involving ultracentrifugation and ion exchange chromatography. Molecular weights of araujiain h II and araujiain h III were 23,718 and 23546 (mass spectrometry), respectively.
Walter David Obregón +5 more
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Inhibition of interleukin 1-stimulated cartilage proteoglycan degradation by a lipophilic inactivator of cysteine endopeptidases [PDF]
Biochemical Journal, 1992 Inactivators of cysteine endopeptidases were tested as inhibitors of the cytokine-stimulated release of proteoglycan from cartilage. The test system consisted of bovine nasal septum cartilage maintained in organ culture, and the stimulus was provided by recombinant human interleukin 1 alpha.
David J. Buttle +3 more
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Heparin Modulates the Endopeptidase Activity of Leishmania mexicana Cysteine Protease Cathepsin L-Like rCPB2.8 [PDF]
PLoS ONE, 2013 Cysteine protease B is considered crucial for the survival and infectivity of the Leishmania in its human host. Several microorganism pathogens bind to the heparin-like glycosaminoglycans chains of proteoglycans at host-cell surface to promote their attachment and internalization.
Wagner Alves de Souza Júdice +11 more
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A Cysteine Endopeptidase (“Dionain”) Is Involved in the Digestive Fluid ofDionaea muscipula(Venus’s Fly-trap) [PDF]
Bioscience, Biotechnology, and Biochemistry, 2011 The carnivorous plant Dionaea muscipula (Venus's flytrap) secretes proteinases into the digestive fluid to digest prey proteins. In this study, we obtained evidence that the digestive fluid contains a cysteine endopeptidase, presumably belonging to the papain family, through inhibitor studies and partial amino acid sequencing of the major SDS-PAGE band
Kenji Takahashi +6 more
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Endopeptidase PepO Regulates the SpeB Cysteine Protease and Is Essential for the Virulence of Invasive M1T1 Streptococcus pyogenes [PDF]
Journal of Bacteriology, 2018 ABSTRACT Streptococcus pyogenes (group A Streptococcus [GAS]) causes a wide range of human infections. The pathogenesis of GAS infections is dependent on the temporal expression of numerous secreted and surface-associated virulence factors that interact with host proteins.
Stephan Brouwer +6 more
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Identification of the active site of legumain links it to caspases, clostripain and gingipains in a new clan of cysteine endopeptidases [PDF]
FEBS Letters, 1998 We show by site‐directed mutagenesis that the catalytic residues of mammalian legumain, a recently discovered lysosomal asparaginycysteine endopeptidase, form a catalytic dyad in the motif His‐Gly‐spacer‐Ala‐Cys. We note that the same motif is present in the caspases, aspartate‐specific endopeptidases central to the process of apoptosis in animal cells,
Jinq-May Chen +3 more
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Frontiers in Immunology, 2023 Glioblastoma is the most common primary malignant tumor of the central nervous system, which has the characteristics of strong invasion, frequent recurrence, and rapid progression.
Hao Liu +19 more
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Frozen fresh blood plasma preserves the functionality of native human α2-macroglobulin
Scientific Reports, 2023 Human α2-macroglobulin (hα2M) is a large homotetrameric protein involved in the broad inhibition of endopeptidases. Following cleavage within a bait region, hα2M undergoes stepwise transitions from its native, expanded, highly flexible, active ...
Soraia R. Mendes +2 more
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