Results 141 to 150 of about 11,913 (180)

The pH dependence of the thermodynamics of the interaction of 3'-cytidine monophosphate with ribonuclease A

Biochemistry, 1975
The apparent free energy (deltaGapp) and enthalpy changes (deltaHB) associated with the interaction of 3'-cytosine monophosphate (3'-CMP) and ribonuclease A (RNase) are reported for the pH range 4--9, T = 25 degrees, mu = 0.05. The pH dependence of deltaGapp and deltaHB has been interpreted in terms of coupled ionization of histidine residues 12, 48 ...
M, Flogel, R L, Biltonen
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Investigations on the Occurrence of Cytidine 3′,5′ Monophosphate (cCMP) in Tissues

Acta Pharmacologica et Toxicologica, 1981
Abstract: A specific and sensitive radioimmunoassay (RIA) for the detection of cCMP was developed. Antibodies were prepared by immunization of rabbits with succinyl cCMP conjugated to human albumin. In the RIA commercially available 3H‐cCMP was used as labeled antigen.
J E, Wikberg, G B, Wingren
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In vivo incorporation of cytidine-monophosphate-ciliatine into rat liver lipids

Biochimie, 1975
1. In vivo this investigation was carried out in order to compare the incorporation into rat lipids of free [1,2-minus 14C]-ciliatine and CMP-[1,2-minus 14C]-ciliatine which is the precursor in phosphonolipid biosynthesis. 2. The incorporation of the radioactivity from CMP-[1,2-minus 14C]-ciliatine took place more rapidly than that from free [1,2-minus
M, Tamari, A, Cassaigne, A M, Lacoste, E, Neuzil   +3 more
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Interaction of cytidine 3'-monophosphate and uridine 3'-monophosphate with ribonuclease A at the denaturation temperature

Biochemistry, 1988
Differential scanning calorimetry (DSC) measurements were performed on the thermal denaturation of ribonuclease a and ribonuclease a complexed with an inhibitor, cytidine or uridine 3'-monophosphate, in sodium acetate buffered solutions. Thermal denaturation of the complex results in dissociation of the complex into denatured ribonuclease a and free ...
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Cytidine monophosphate as substrate for the electron microscopic visualization of alkaline phosphatase activity

Histochemie, 1967
Since in histochemical reactions the liver alkaline phosphatase discloses only low activity with respect to beta-glycerophosphate, the authors have replaced the latter by cytidine monophosphate. With this substrate they obtained much better results: greater sensibility permitting to reduce the incubation time, and much more precise localization of the ...
H, Oledzka-Slotwińska, J, Creemers, V, Desmet   +2 more
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Enzyme immunoassay for cytidine 3′,5′-cyclic monophosphate (cyclic CMP)

Immunopharmacology, 1982
An enzyme immunoassay for cytidine 3',5'-cyclic monophosphate (cyclic CMP) is presented. This assay is based upon the principles of competitive reaction and the double antibody solid phase method. Succinyl cyclic CMP-human serum albumin conjugate was injected into rabbits.
I, Yamamoto, T, Takai, J, Tsuji
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Cytidine 3′,5′-Monophosphate (Cyclic CMP) Formation in Mammalian Tissues

Science, 1977
Mammalian tissues possess the capacity to synthesize cytidine 3′,5′-monophosphate (cyclic CMP) via the enzymatic conversion of cytidine 5′-triphosphate to cyclic CMP by cytidylate cyclase. Cyclic CMP formation occurs best in the presence of manganese or iron, at neutral p H, at 37°C, in the absence of detergents ...
S Y, Cech, L J, Ignarro
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The effects of cytidine monophosphate on the regeneration of sialoproteins on the surface of cultured lymphoma cells

Biochemical and Biophysical Research Communications, 1977
Abstract A new approach was used to evaluate the role of surface sialyl transferase activity in the regeneration of surface sialic acid (SSA) on cultured lymphoma cells (Raji). Cells which were made deficient in SSA by neuraminidase treatment were incubated for 18 hours in medium containing CMP, a potent inhibitor of surface sialyl transferase ...
R D, Maca, A, Hakes
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Radioimmunological identification and measurement of cytidine 3′, 5′-monophosphate in rat tissues

Biochemical and Biophysical Research Communications, 1978
Abstract A radioimmunoassay for cyclic CMP3 is presented. Separation of cyclic CMP from other cyclic nucleotides and conversion to its 2′ O-acyl derivative was found necessary to achieve the specificity and sensitivity required. Low but easily measurable concentrations of cyclic CMP were found in rat liver spleen and kidney.
H L, Cailla, D, Roux, M, Delaage, C, Goridis   +3 more
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Radioimmunoassay of cytidine 3′,5′ monophosphate (cCMP) I. Development of the assay

Biochemical and Biophysical Research Communications, 1979
Abstract A method is presented for the production of reagents for a radioimmunoassay for cCMP. cCMP was succinylated at the 2′0 position with [1,4 14C] succinic anhydride, and the monosuccinyl cCMP coupled to Keyhole limpet hemocyanin and injected into rabbits.
B E, Murphy, J E, Stone
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