A mouse model for improving cell survival of bisected cattle embryos
Morula and blastocyst stage embryos recovered from B6D2F 1 mice were bisected with a metal microlabde in M2 medium with or without sucrose and/or cytochalasin B supplementation.
P. BREDBACKA
doaj
The Outcome of Intracellular Selenomonas sputigena and its Impact on Gingival Keratinocytes
ABSTRACT Selenomonas sputigena is an understudied oral pathobiont associated with periodontitis and dental caries. We recently demonstrated that S. sputigena binds to gingival epithelial cells (GECs), where afterwards the bacterium causes robust in vitro pro‐inflammatory cytokine production and migration of monocytes and neutrophils.
Colin G. Hawkes +6 more
wiley +1 more source
A mouse model for improving cell survival of bisected cattle embryos
Morula and blastocyst stage embryos recovered from B6D2F_1 mice were bisected with a metal microblade in M2 medium with or without sucrose and/or cytochalasin B supplementation.
Peter Bredbacka
doaj
Outer Membrane Vesicles Mediate the Secretion and Nuclear Trafficking of a Bacterial Nucleomodulin
(A) We propose that Helicobacter pylori secretes a nucleomodulin, Tumour Necrosis Factor‐α‐inducing protein (Tipα), predominantly via extracellular vesicles (EVs), also known as outer membrane vesicles, rather than as free protein. (B) EVs carrying Tipα enter eukaryotic cells by endocytosis and independently of surface‐expressed nucleolin, then traffic
Jack K. Emery +14 more
wiley +1 more source
Molecular basis for a pore block of Tentonin 3 expressed in HEK293 cells by a conopeptide, NMB‐1
Background and Purpose Tentonin 3 (TTN3/TMEM150C) is a mechanosensitive ion channel that plays critical roles in mechanotransduction processes. TTN3 forms a tetramer with a predicted rectangular shape and a central pore. A conotoxin ρ‐TIA and its synthetic analog, noxious mechanosensation blocker 1 (NMB‐1), were initially developed to inhibit slowly ...
Sujin Lim +10 more
wiley +1 more source
Mechanical Force Promotes Mitochondrial Transfer From Macrophages to BMSCs to Enhance Bone Formation
Mechanical tension activates Drp1‐mediated mitochondrial fission in macrophages, facilitating the release of Mito‐EVs that are subsequently transferred to BMSCs. Additionally, the CD200R‐CD200 interaction enhances the uptake of these mechanically stimulated macrophage‐derived Mito‐EVs by BMSCs, ultimately promoting osteogenic differentiation.
Yingyi Li +8 more
wiley +1 more source
Artificial Extracellular Vesicles Generated from T Cells Using Different Induction Techniques
Cell therapy is at the forefront of biomedicine in oncology and regenerative medicine. However, there are still significant challenges to their wider clinical application such as limited efficacy, side effects, and logistical difficulties.
Ekaterina A. Zmievskaya +8 more
doaj +1 more source
Deciphering the biosynthetic pathways of lichen acids
Summary Depsides and depsidones are polyketide‐derived lichen acids widely distributed in lichen thalli, yet the biosynthetic gene clusters (BGCs) responsible for their production remain poorly understood. To address this gap, we investigated the diversity and evolutionary relationships of polyketide BGCs in lichens.
Wonyong Kim +11 more
wiley +1 more source
Binary specification of nerve cord and notochord cell fates in ascidian embryos [PDF]
In the ascidian embryo, the nerve cord and notochord of the tail of tadpole larvae originate from the precursor blastomeres for both tissues in the 32-cell-stage embryo. Each fate is separated into two daughter blastomeres at the next cleavage.
Makabe, Kazuhiro W. +3 more
core
Degradation of connective tissue matrices by macrophages. III. Morphological and biochemical studies on extracellular, pericellular, and intracellular events in matrix proteolysis by macrophages in culture. [PDF]
We have shown that macrophages in culture degrade the glycoproteins and amorphous elastin of insoluble extracellular matrices. Ultrastructural observation of the macrophage-matrix interaction revealed that connective tissue macromolecules were ...
Bainton, DF, Jones, PA, Werb, Z
core

