Frontiers in Immunology, 2022 Mass cytometry has revolutionized immunophenotyping, particularly in exploratory settings where simultaneous breadth and depth of characterization of immune populations is needed with limited samples such as in preclinical and clinical tumor ...
Akshay Iyer +8 more
doaj +3 more sources
Immune profiling in orthopaedic diseases using mass cytometry: a narrative review of applications and future directions [PDF]
Bone & Joint ResearchAims: Musculoskeletal (MSK) diseases involve complex immune mechanisms. Conventional flow cytometry is restricted by spectral overlap, limiting multidimensional immune characterization.
Yosuke Susuki +10 more
doaj +2 more sources
Validation of CyTOF Against Flow Cytometry for Immunological Studies and Monitoring of Human Cancer Clinical Trials [PDF]
Frontiers in Oncology, 2019 Flow cytometry is a widely applied approach for exploratory immune profiling and biomarker discovery in cancer and other diseases. However, flow cytometry is limited by the number of parameters that can be simultaneously analyzed, severely restricting ...
Ramy Gadalla +21 more
doaj +2 more sources
Rhenium‐Based β‐Galactosidase Probe Allows Senescence Profiling by Mass Cytometry [PDF]
Angewandte Chemie, Volume 138, Issue 42, 12 October 2026.Senescence‐associated β‐galactosidase (SA‐β‐Gal) activity is a widely accepted biomarker of cellular senescence, yet its integration with other senescence markers has been limited by available detection methods. Here, we introduce ReGal, a mass cytometry‐compatible SA‐β‐Gal activity probe that can be multiplexed with established markers of senescence ...
Adam Tam +4 more
wiley +3 more sources
CyTOF-Enabled Analysis Identifies Class-Switched B Cells as the Main Lymphocyte Subset Associated With Disease Relapse in Children With Idiopathic Nephrotic Syndrome [PDF]
Frontiers in Immunology, 2021 B cell depleting therapies permit immunosuppressive drug withdrawal and maintain remission in patients with frequently relapsing nephrotic syndrome (FRNS) or steroid–dependent nephrotic syndrome (SDNS), but lack of biomarkers for treatment failure.
Miguel Fribourg +13 more
doaj +2 more sources
Parameter optimization for stable clustering using FlowSOM: a case study from CyTOF [PDF]
Frontiers in ImmunologyHigh-dimensional cell phenotyping is a powerful tool to study molecular and cellular changes in health and diseases. CyTOF enables high-dimensional cell phenotyping using tens of surface and intra-cellular markers. To utilize the full potential of CyTOF,
Weiyang Tao +3 more
doaj +2 more sources
The CyTOF dataset from aGVHD patients receiving liver transplantation
, 2022 Mass cytometry (CyTOF) was adopted for systematic and deep phenotyping of the PBMCs from the aGVHD (N=3) and control (N=10) patients.
Li Yiyang
core +3 more sources
Journal of Translational Medicine, 2018 Background Mass cytometry, or CyTOF (Cytometry by Time-of-Flight), permits the simultaneous detection of over 40 phenotypic and functional immune markers in individual cells without the issues of spectral overlap seen in traditional flow cytometry ...
Christine M. D. Lingblom +4 more
doaj +2 more sources
An R-based reproducible and user-friendly preprocessing pipeline for CyTOF data [PDF]
F1000Research, 2020 Mass cytometry (CyTOF) has become a method of choice for in-depth characterization of tissue heterogeneity in health and disease, and is currently implemented in multiple clinical trials, where higher quality standards must be met.
Chevrier, Stéphane +7 more
core +5 more sources
The use of mass cytometry (CyTOF) to evaluate the cellular uptake of stable radiopharmaceutical surrogates in single cells: a proof-of-concept study [PDF]
EJNMMI Radiopharmacy and ChemistryBackground Current methods for assessing radiopharmaceutical uptake are based on scintillation counting (e.g., γ-counting) or imaging techniques, such as positron emission tomography (PET) and single photon emission computed tomography (SPECT), which ...
Miguel Gómez-Sánchez +5 more
doaj +2 more sources