Results 101 to 110 of about 52,861 (267)

Beyond Structure: Methylation Fine‐Tunes Stability and Folding Kinetics of bcl2Mid G‐Quadruplex

Angewandte Chemie, EarlyView.
The introduction of a single 5‐methylcytosine into a G‐rich sequence originating from the B‐cell lymphoma 2 (BCL2) gene promoter affects both the folding kinetics and thermodynamics of the two G4 structures and thus plays a crucial role in regulating G4 folding pathways, which has significant implications for the control of gene expression.
Nataša Medved, Mirko Cevec, Uroš Javornik, Jurij Lah, San Hadži, Janez Plavec   +5 more
wiley   +2 more sources

Hidden complexities in the unfolding mechanism of a cytosine-rich DNA strand [PDF]

arXiv, 2011
We investigate the unfolding pathway of a cytosine-rich DNA hairpin structure via Molecular Dynamics simulations. Our results indicate a hidden complexity present in the unfolding process. W e show that this complex behavior arises due to non-stochastic contributions which have an important impact on the description of the dynamics in terms of ...
arxiv  

Transcriptional Similarity in Couples Reveals the Impact of Shared Environment and Lifestyle on Gene Regulation through Modified Cytosines [PDF]

, 2016
Gene expression is a complex and quantitative trait that is influenced by both genetic and non-genetic regulators including environmental factors. Evaluating the contribution of environment to gene expression regulation and identifying which genes are more likely to be influenced by environmental factors are important for understanding human complex ...
arxiv   +1 more source

Paternal Obesity‐Induced H3K27me3 Elevation Leads to MANF‐Mediated Transgenerational Metabolic Dysfunction in Female Offspring

Advanced Science, EarlyView.
Paternal lifestyle and environmental exposures play a critical role in the offspring's health. The present study established a paternal obesity model and demonstrated that elevated H3K27me3 levels in sperm persist through the 8‐cell embryo stage. This epigenetic modification triggers transgenerational decrease of MANF, inducing ER stress and activating
Yajun Shi, Weisheng Li, Xi Yu, Yan Zhao, Dan Zhu, Yueyang Song, Zejun Zhao, Yannan Gu, Bin Wei, Lingjun Li, Dongyi Yu, Pengjie Zhang, Qinqin Gao, Miao Sun   +13 more
wiley   +1 more source

Enzymatic approaches and bisulfite sequencing cannot distinguish between 5-methylcytosine and 5-hydroxymethylcytosine in DNA

BioTechniques, 2010
DNA cytosine methylation (5mC) is highly abundant in mammalian cells and is associated with transcriptional repression. Recently, hydroxymethylcytosine (hmC) has been detected at high levels in certain human cell types; however, its roles are unknown ...
Colm Nestor, Alexey Ruzov, Richard R. Meehan, Donncha S. Dunican   +3 more
doaj   +1 more source

Mathematical framework of epigenetic DNA methylation in gene body Arabidopsis [PDF]

arXiv, 2012
In aiming to explain the establishment, maintenance and stability of methylation pattern in gene body of Arabidopsis we propose here a theoretical framework for understanding how the methylated and unmethylated states of cytosine residues are maintained and transmitted during DNA replication.
arxiv  

Cytosine deamination and the precipitous decline of spontaneous mutation during Earth's history

Proceedings of the National Academy of Sciences of the United States of America, 2016
Significance Cytosine deamination appears to be largely responsible for spontaneous mutations in the modern world. Because of its sensitivity to temperature (Q10 = 4), that reaction would have furnished a mechanism for rapid evolution on a warm earth. As
C. A. Lewis, Jesse Crayle, Shuntai Zhou, R. Swanstrom, R. Wolfenden   +4 more
semanticscholar   +1 more source

Enhancing Prime Editing Efficiency Through Modulation of Methylation on the Newly Synthesized DNA Strand and Prolonged Expression

Advanced Science, EarlyView.
Prime editors (PEs) enhance gene editing precision but face repair mechanism limitations. DNA methylation (CpG sites) and de novo methyltransferases (DNMT3A/DNMT3B) boost PE efficiency. We developed an episomal PE system (epiPE) using EBNA1/oriP to sustain expression and methylation.
Xiaosong Han, Xianghua Xu, Youcai Xiong, Guangxing Zhao, Ruigao He, Yinyu Su, Sheng Li, Changzhi Zhao, Xiaoning Xi, Yunxia Zhao, Xuewen Xu, Shengsong Xie, Heng Wang, Xinyun Li, Shuhong Zhao, Jinxue Ruan   +15 more
wiley   +1 more source

Metal‐free phthalimide‐labeled peptide nucleic acids for electrochemical biosensing applications

Electrochemical Science Advances, Volume 2, Issue 6, December 2022., 2022
Abstract Peptide nucleic acids (PNAs) are neutral mimics of natural DNA and RNA biopolymers that have caught the attention of researchers working on the identification of specific sequences of nucleobases in DNA/RNA strands. For this purpose, specific analytical protocols need to be developed to optimize the nucleic acid recognition ability of PNAs ...
Mirko Magni, Sergio Dall'Angelo, Clara Baldoli, Emanuela Licandro, Luigi Falciola, Patrizia R. Mussini   +5 more
wiley   +1 more source

Epigenetic effects of cytosine derivatives are caused by their tautomers in Hoogsteen base pairs [PDF]

arXiv, 2014
Deoxycitidine in solution exists as two tautomers one of which is an uncanonical imino one. The latter can dominate with such derivatives as 5-methyl, 5-hydroxymethyl- and 5-formylcytosine. The imino tautomer potentially is able to form a hoosteen GC base pair. To detect such pair, it is suggested to use 1H15N NMR.
arxiv