Results 91 to 100 of about 233,386 (297)

Activity on non-methylated DNA limits the use of endonuclease MspJI for epigenetic analyses

All Results Journals: Biol, 2018
Cytosine methylation of DNA in mammals has been associated with both physiological and pathological changes in gene-expression. DNA treatment with methylation sensitive and/or dependent restriction enzymes, followed by PCR amplification is a widely used ...
María Belén Jerez, Maximiliano Juri Ayub   +1 more
doaj  

{BiQ} Analyzer {HiMod}: An Interactive Software Tool for High-throughput Locus-specific Analysis of 5-Methylcytosine and its Oxidized Derivatives [PDF]

, 2014
Recent data suggest important biological roles for oxidative modifications of methylated cytosines, specifically hydroxymethylation, formylation and carboxylation. Several assays are now available for profiling these DNA modifications genome-wide as well
Becker, D., Bock, C., Ebert, P., Lengauer, T., Lutsik, P., Walter, J.   +5 more
core   +2 more sources

Therapy for Myhre Syndrome: Goals, Misconceptions, and Current Agents

American Journal of Medical Genetics Part C: Seminars in Medical Genetics, EarlyView.
ABSTRACT Myhre Syndrome (MYHRS, MIM #139210) is a rare, multisystem connective tissue disorder caused by recurrent heterozygous gain‐of‐function pathogenic variants in the SMAD4 gene, a key player in TGF‐β signaling and a regulator of extracellular matrix homeostasis.
Alessandro De Falco, Alfonso Manuel D'Alessio, Nicola Brunetti‐Pierri   +2 more
wiley   +1 more source

One‐step generation of heritable mitochondrial DNA multiplex‐engineered rats using DddA‐derived cytosine base editor

Animal Models and Experimental Medicine, EarlyView.
We established that mixed DdCBE microinjection is an efficient, heritable, and precise strategy for generating multiplex mtDNA mutant rats. This advancement significantly expands the utility of DdCBEs for mitochondrial disease modeling, providing a robust platform for exploring the pathogenic mechanisms of complex mtDNA mutations and developing ...
Xu Zhang, Keru Li, Lei Tan, Wei Chen, Shan Gao, Chenyang Liu, Shuo Pan, Jiayue He, Ning Liu, Gefan Wan, Wei Dong, Weining Kong, Bin Shen, Xiaolong Qi, Yuanwu Ma   +14 more
wiley   +1 more source

The amniote-conserved DNA-binding domain of CGGBP1 restricts cytosine methylation of transcription factor binding sites in proximal promoters to regulate gene expression

BMC Genomic Data
CGGBP1 is a GC-rich DNA-binding protein which is important for genomic integrity, gene expression and epigenome maintenance through regulation of CTCF occupancy and cytosine methylation.
Ishani Morbia, Praveen Kumar, Aditi Lakshmi Satish, Akanksha Mudgal, Subhamoy Datta, Umashankar Singh   +5 more
doaj   +1 more source

Enzymatic approaches and bisulfite sequencing cannot distinguish between 5-methylcytosine and 5-hydroxymethylcytosine in DNA

BioTechniques, 2010
DNA cytosine methylation (5mC) is highly abundant in mammalian cells and is associated with transcriptional repression. Recently, hydroxymethylcytosine (hmC) has been detected at high levels in certain human cell types; however, its roles are unknown ...
Colm Nestor, Alexey Ruzov, Richard R. Meehan, Donncha S. Dunican   +3 more
doaj   +1 more source

Recognition of abasic sites and single base bulges in DNA by a metalloinsertor [PDF]

, 2009
Abasic sites and single base bulges are thermodynamically destabilizing DNA defects that can lead to cancerous transformations if left unrepaired by the cell.
Barton, Jacqueline K., Boland, Jennifer A., Zeglis, Brian M.   +2 more
core   +1 more source

Ribozyme für die RNA‐katalysierte Methylierung und Markierung von RNA

Angewandte Chemie, EarlyView.
Ribozyme sind vielseitige Werkzeuge für die präzise Modifikation von RNA. Das Reaktionsspektrum in‐vitro‐selektierter Ribozyme reicht vom Einbau funktioneller Gruppen und Fluorophoren bis zu natürlichen RNA‐Methylierungen. Dieser Übersichtsartikel präsentiert Cofaktor‐abhängige Ribozyme für die positionsgenaue Markierung und Modifikation von RNA ...
Carolin P. M. Scheitl, Claudia Höbartner   +1 more
wiley   +1 more source

Purine and pyrimidine bases as growth substances for lactic acid bacteria [PDF]

, 1941
In 1936 Richardson (1) showed that uracil was essential for the anaerobic growth of Staphylococcus aureus, but not for aerobic growth of the same organism.
Mitchell, Herschel K., Snell, Esmond E.
core  

Tight-binding parameters for charge transfer along DNA

, 2009
We systematically examine all the tight-binding parameters pertinent to charge transfer along DNA. The $\pi$ molecular structure of the four DNA bases (adenine, thymine, cytosine, and guanine) is investigated by using the linear combination of atomic ...
A. Holmen, A. Migliore, A. Rakitin, A.A. Voityuk, A.A. Voityuk, A.F. Fucaloro, A.J. Storm, A.L. Sobolewski, A.O. Colson, B. Giese, B. Mennucci, C. Simserides, C.A. Sprecher, C.J. Burrows, Ch. Adesi, Ch. Adesi, D. Dougherty, D. Hennig, D. Hennig, D. Porath, D. Varsano, D. Voet, D.B. Hall, D.W. Miles, E. Diaz, E. Meggers, F. Zaloudek, F.C. Grozema, G. Kalosakas, G. Kalosakas, G. Kalosakas, G. Kalosakas, G. Lauer, G.P. Triberis, G.P. Triberis, H. Mehrez, H. Sugiyama, H. Zhang, H.W. Fink, J. Lin, J. Lin, J. Lorentzon, J.B. MacNaughton, J.C. Slater, K. Keren, K. Raksanyi, K. Senthilkumar, K.-H. Yoo, L. Blancafort, L. Cai, L. G. D. Hawke, L.B. Clark, L.B. Clark, L.G.D. Hawke, L.G.D. Hawke, M. Fuentes-Cabrera, M. Fuentes-Cabrera, M. Fuentes-Cabrera, M. Hutter, M. Menon, M. Menon, M.K. Shukla, M.K. Shukla, M.K. Shukla, M.K. Shukla, M.P. Fülscher, M.P. Fülscher, M.R. Arkin, M.R. Singh, N. Ismail, N. Lathiotakis, N. Rosch, N.C. Seeman, N.S. Hush, P. Maniadis, P. Maniadis, P. Maragakis, P. Tran, P.B. Woiczikowski, P.J. Dandliker, P.J. de Pablo, P.T. Henderson, R. Bruinsma, R. Di Felice, R.G. Endres, S. Komineas, S. Urano, S.R. Rajski, S.S. Mallajosyula, S.V. Rakhmanova, T. Cramer, T. Fleig, T. Kubar, T. Yamada, W. Voelter, W. Zhang, W.C. Brunner, X. Li, X. Li, Y. Matsuoka, Y. Zhang, Y.-J. Ye, Y.A. Berlin, Y.A. Berlin, Z. Kutnjak, Z.G. Yu   +105 more
core   +1 more source