Results 71 to 80 of about 133,095 (262)

Dynamic Operations in Macromolecular Data Storage

Angewandte Chemie, EarlyView.
With the exponential growth of digital data and the limitations of conventional silicon‐based storage and computing technologies, macromolecular data storage, the practice of encoding digital information within large, complex molecules like DNA or synthetic polymers, has emerged as a scalable and sustainable alternative to traditional digital ...
Jakub Ossowski, Oskar Affenberger, Jean‐François Lutz, Maria Nerantzaki   +3 more
wiley   +2 more sources

Continuous Evolution System Based on Toxin–Antitoxin System Combined with Base Deaminase Accelerates the In Vivo Modification of Target Proteins

Advanced Science, EarlyView.
The promoted Escherichia coli‐assisted continuous evolution (PEACE) system operates through a stringent three step genetic circuit: target gene mutation via a deaminase–T7 RNA polymerase (T7 RNAP) fusion, coupling variant function to antitoxin expression, and growth based selection of surviving cells.
Xinyu Zhang, Zhanzhi Liu, Ying Xu, Mingjing Sun, Guangzhen Zhou, Deming Rao, Xiaoqian Chen, Sheng Wang, Zhigang Li, Yan Huang, Jing Wu   +10 more
wiley   +1 more source

Biosynthesis and Genetic Tuning of Guanine Crystals via Metabolic Engineering of Yeast

Angewandte Chemie, EarlyView.
We demonstrate how yeast cells can be harnessed as cellular factories for the synthesis of optically functional guanine crystals, where genetic editing is used to tune crystal size, order and optical properties. This demonstrates a powerful new biosynthetic approach for the development of sustainable and biocompatible optical pigments, which utilizes ...
Jerome N. Janssen, Yahel Fishman, Yi Li, Avital Wagner, Mariela J. Pavan, Zohar Paleiov, Tali Lemcoff, Rotem Lichtenstein‐Wolfheim, Alexander Upcher, Einat Nativ‐Roth, Amir Aharoni, Benjamin A. Palmer   +11 more
wiley   +2 more sources

Compact 9dBEs Enable Efficient and Precise Genome Editing in Mammalian Cells and In Vivo

Advanced Science, EarlyView.
As a compact type II‐D system, the Cas9d‐based platform holds great potential for in vivo applications. Through rational engineering, its derived base editors (9dBEs) enable efficient disease modeling while facilitating single‐vector AAV delivery for in vivo genome editing. These miniature tools offer a robust strategy for basic research and biomedical
Qingquan Xiao, Zhijin Tian, Luqi Weng, Kun Xu, Dingyi Han, Zhaowei Wu, Junnan Li, Shangpu Li, Qijing Zhang, Xiaohui Zhao, Guoling Li, Yinghui Wei, Zhen Gu   +12 more
wiley   +1 more source

Biotin‐ and Chloroalkane‐Modified Nucleotides for Dual Display of Proteins on DNA Through Streptavidin and Halotag Binding

Angewandte Chemie, EarlyView.
Modified 2’‐deoxy‐7‐deazaadenosine bearing a chlorohexyl moiety and 2’‐deoxycytidine nucleotides bearing biotin were used in the enzymatic synthesis of dually modified dsDNAs that served as scaffolds for display of halotag and monomeric streptavidin proteins.
Giammarco Liuni, Lenka Poštová Slavětínská, Miroslav Hájek, Veronika Raindlová, Michal Hocek   +4 more
wiley   +2 more sources

NICE: A Two‐Step Non‐Invasive Framework for Embryo cfDNA Read Enrichment and Quality Assessment

Advanced Science, EarlyView.
The non‐invasive NICE framework, built on an ensemble stacking machine learning model, prioritizes embryos by analyzing cell‐free DNA from spent culture medium. By integrating multimodal signals, including genomic and epigenetic profiles, this automated approach standardizes morphological assessment without human bias, paving the way for more precise ...
Xueya Zhou, Shu Ding, Zhenyi Zhang, Qiaoling Shangguan, Jie Qiao, Peijie Zhou, Yidong Chen   +6 more
wiley   +1 more source

An Enzymatic Platform for Late‐Stage (Radio)isotope Labelling of Oligonucleotides With Methyltransferases

Angewandte Chemie, EarlyView.
State‐of‐the‐art synthesis of radiolabelled oligonucleotides involves multi‐step synthesis with high costs, extended timelines and significant radioactive waste generation. Herein, a dual‐methyltransferase platform enables late‐stage, site‐specific labelling, overcoming these limitations through reduced costs, faster turnaround and minimal radioactive ...
Christopher R. B. Swanson, Ulrich Kauhl, Martin R. Edelmann, Florian P. Seebeck   +3 more
wiley   +2 more sources

Density Functional Theory Investigation on Muon Hyperfine Interaction in Methylated Guanine-Cytosine Double-Strand DNA

Journal of Mathematical and Fundamental Sciences
The aims of this study were to determine the most likely Mu trapping sites based on total energy consideration as well as identifying the associated muon hyperfine coupling constant of 1, 2, and 3 methylated guanine-cytosine base pair double-strand DNA.
Ammaina Jamaludin, Shukri Sulaiman, Wan Nuradhilah Zaharim, Ang Lee Sin   +3 more
doaj   +3 more sources

p300‐Mediated H3K18 Lactylation Drives Radiation‐Induced Pulmonary Fibrosis via VIRMA‐Dependent m6A Modification of GATA3

Advanced Science, EarlyView.
Radiation triggers glycolytic reprogramming in alveolar epithelial type II cells, causing pathological lactate accumulation. This metabolic stress drives p300‐mediated H3K18 lactylation at the VIRMA promoter, activating transcription. Elevated VIRMA enriches m6A on GATA3 mRNA, which YTHDF1 stabilizes, ultimately driving epithelial–mesenchymal ...
Junxuan Yi, Mingwei Wang, Duo Yu, Mingqi Zhao, Xinyan Wang, Shiqi Li, Xiaowen Han, Wei Wei, Shunzi Jin   +8 more
wiley   +1 more source

Cytosine base editor-DNA binding domain fusions for editing window modulation in the RNP format

BMC Biotechnology
Summary Base editing technologies allow for the precise and efficient installation of defined nucleotide substitutions into a target genome without the introduction of double strand breaks or DNA templates.
Erin Brettmann, Fuqiang Chen, Stephen Beishir, Graeme Garvey   +3 more
doaj   +1 more source