Results 11 to 20 of about 440 (100)

Safety evaluation of the food enzyme d‐tagatose 3‐epimerase from the genetically modified Escherichia coli strain PS‐Sav‐001 [PDF]

EFSA Journal, 2023
The food enzyme d‐tagatose 3‐epimerase (EC 5.1.3.31) is produced with the genetically modified Escherichia coli strain PS‐Sav‐001 by SAVANNA Ingredients GmbH. The genetic modifications do not give rise to safety concerns.
EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP), Claude Lambré, José Manuel Barat Baviera, Claudia Bolognesi, Pier Sandro Cocconcelli, Riccardo Crebelli, David Michael Gott, Konrad Grob, Evgenia Lampi, Marcel Mengelers, Alicja Mortensen, Gilles Rivière, Inger‐Lise Steffensen, Christina Tlustos, Henk Van Loveren, Laurence Vernis, Holger Zorn, Boet Glandorf, Lieve Herman, Magdalena Andryszkiewicz, Ana Gomes, Yi Liu, Silvia Peluso, Andrew Chesson   +23 more
doaj   +3 more sources

Production, purification, characterization, and safety evaluation of constructed recombinant D-psicose 3-epimerase [PDF]

Microbial Cell Factories
Background D-psicose 3-epimerase (DPEase) is a potential catalytic enzyme for D-psicose production. D-psicose, also known as D-allulose, is a low-calorie sweetener that has gained considerable attention as a healthy alternative sweetener due to its ...
Nisit Watthanasakphuban, Pimsiriya Srila, Phitsanu Pinmanee, Charatda Punvittayagul, Nopphon Petchyam, Boontiwa Ninchan   +5 more
doaj   +2 more sources

Optimization of fermentation conditions for whole cell catalytic synthesis of D-allulose by engineering Escherichia coli [PDF]

Scientific Reports
D-allulose/D-psicose is a significant rare sugar with broad applications in the pharmaceutical, food, and other industries. In this study, we cloned the D-allulose 3-epimerase (DPEase) gene from Arthrobacter globiformis M30, using pET22b as the vector ...
Haoran Liu, Kang Xu, ShuQi Sun, Yinbiao Wan, Bojia Zhang, Yang Song, Chuanzhuang Guo, Songsen Sui, Ruiming Wang, Piwu Li, Junqing Wang, Zhenshang Xu, Ting Wang   +12 more
doaj   +2 more sources

A food-grade expression system for d-psicose 3-epimerase production in Bacillus subtilis using an alanine racemase-encoding selection marker. [PDF]

Bioresour Bioprocess, 2017
Chen J, Jin Z, Gai Y, Sun J, Zhang D.
europepmc   +2 more sources

Production of recombinant D-allulose 3-epimerase utilizing an auto-induction approach in fermentor cultures suitable for industrial application. [PDF]

PLoS One
D-Allulose 3-epimerase (DAEase) is the key enzyme catalyzing D-fructose to catalyze into D-allulose, a rare sugar in foods, which has lately drawn increasing worldwide attention owing to its possible health advantages and application as a substitute ...
Lischer K, Laksmi FA, Nugraha Y, Helbert, Az-Zahra F, Herawan D, Juanssilfero AB, Wibowo DS, Ramadhan KP, Nuryana I, Ali MSM.   +10 more
europepmc   +3 more sources

Assessment of the potential risks in SD rats gavaged with genetically modified yeast containing the cp4-epsps gene. [PDF]

Front Vet Sci
IntroductionDespite the absence of definitive evidence indicating that the cp4-epsps gene and its resultant recombinant proteins have significant harmful effects on either human or animal health, the safety assessment of genetically modified (GM) crops ...
Bi B, Fu X, Jian X, Zhang Y, Jiang Y, Zhou W, Zhao H.   +6 more
europepmc   +3 more sources

Construction of hyperthermostable d-allulose 3-epimerase from Arthrobacter globiformis M30 using the sequence information from Arthrobacter psychrolactophilus. [PDF]

FEBS Open Bio
d‐Allulose can be produced from d‐fructose by d‐allulose 3‐epimerase. Based on sequence homology information, we successfully engineered thermostable mutants with the protein engineering method. By integrating positive mutations, we constructed an enzyme that exhibits hyperthermostability without a loss in the activity.
Shimada K, Ohtani K, Gullapalli PK, Ishikawa K.   +3 more
europepmc   +2 more sources

Safety of D-allulose as a novel food pursuant to Regulation (EU) 2015/2283. [PDF]

EFSA J
Abstract Following a request from the European Commission, the EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA) was asked to deliver an opinion on D‐allulose as a novel food (NF) pursuant to Regulation (EU) 2015/2283. During the risk assessment, the Panel identified a number of data gaps, which needed to be addressed by the applicant ...
EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA), Turck D, Cámara M, Bohn T, Castenmiller J, De Henauw S, Jos Á, Maciuk A, Mangelsdorf I, McNulty B, Naska A, Pentieva K, Siani A, Thies F, Aguilera-Gómez M, Cubadda F, Frenzel T, Heinonen M, Knutsen HK, McArdle HJ, Neuhäuser-Berthold M, Poulsen M, Maradona MP, Schlatter JR, Siskos A, van Loveren H, Multari S, Rossi A, Hirsch-Ernst KI.   +29 more
europepmc   +2 more sources

Non/Low-Caloric Artificial Sweeteners and Gut Microbiome: From Perturbed Species to Mechanisms. [PDF]

Metabolites
Background: Non/low-caloric artificial sweeteners (NAS) are recognized as chemical additives substituting sugars to avoid caloric intake and subsequent sugar-derived diseases such as diabetes and hyperglycemia.
Feng J, Peng J, Hsiao YC, Liu CW, Yang Y, Zhao H, Teitelbaum T, Wang X, Lu K.   +8 more
europepmc   +3 more sources

An Update: Enzymatic Synthesis for Industrial Applications. [PDF]

Angew Chem Int Ed Engl
Biocatalysis has become a sustainable and cost‐competitive alternative to established chemical synthesis, enabling the enzyme‐based production of not only commodity chemicals but (non‐natural) amino acids, (rare) sugars, as well as synthetic nucleotides.
Bayer T, Wu S, Snajdrova R, Baldenius K, Bornscheuer UT.   +4 more
europepmc   +2 more sources