Streamlined production of immobilized D-psicose 3-epimerase via secretion in Pichia pastoris: a new paradigm for industrial D-psicose production. [PDF]
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Co-immobilization of whole cells and enzymes by covalent organic framework for biocatalysis process intensification. [PDF]
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Synthetic Biology Approaches to Enzymology in Food and Agriculture Systems. [PDF]
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Impact of artificial sweeteners and rare sugars on the gut microbiome. [PDF]
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Current Progress and Future Directions of Enzyme Technology in Food Nutrition: A Comprehensive Review of Processing, Nutrition, and Functional Innovation. [PDF]
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D-Allulose, as low-calorie rare sugar, possessed several notable biological activities and was biosynthesized by D-allulose 3-epimerase (DAEase). Here, CcDAE from Clostridium cellulolyticum was successfully immobilization via covalent attachment (RI-CcDAE), and Resin-SpyCatcher/SpyTag-CcDAE modular (DI-CcDAE).
Fuping Lu, Hui-Min Qin
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Abstract • Allulose is an epimer of d -fructose with multiple nutritional functions, which can be biologically synthesized from d -fructose employing d -allulose 3-epimerase (DAE). In this study, NCBI database mining approach was applied to screen novel DAEs, and Novibacillus thermophilus DAE (NtDAE) exhibiting a high activity of 273.9 U/(g ...
Dong-Xu Jia +2 more
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In recent years, with the increasing public health awareness, low-calorie rare sugars have received more attention on a global scale. D-Allulose, the C-3 epimer of D-fructose, is a representative rare sugar. It displays high sweetness and excellent physiological functions, but only provides a caloric value of 0.4 kcal/g. D-Allulose 3-epimerase (DAEase)
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