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International Journal of Biological Macromolecules, 2022
A novel D-allulose 3-epimerase (DAEase) from Arthrobacter psychrolactophilus (Ap DAEase) was first characterized in this study. The enzyme catalyzes the epimerization of d-fructose into a functional rare sugar, D-allulose. Ap DAEase was the first record of DAEase identified as a homotrimer with the molecular weight of its subunit at approximately 34 ...
Fina Amreta Laksmi +2 more
exaly +3 more sources
A novel D-allulose 3-epimerase (DAEase) from Arthrobacter psychrolactophilus (Ap DAEase) was first characterized in this study. The enzyme catalyzes the epimerization of d-fructose into a functional rare sugar, D-allulose. Ap DAEase was the first record of DAEase identified as a homotrimer with the molecular weight of its subunit at approximately 34 ...
Fina Amreta Laksmi +2 more
exaly +3 more sources
Critical Reviews in Biotechnology
D-allulose, an epimer of D-fructose at C-3 position, is a low-calorie rare sugar with favorable physiochemical properties and special physiological functions, which displays promising perspectives in the food and pharmaceutical industries. Currently, D-allulose is extremely sparse in nature and is predominantly biosynthesized through the isomerization ...
Caiming Li +2 more
exaly +3 more sources
D-allulose, an epimer of D-fructose at C-3 position, is a low-calorie rare sugar with favorable physiochemical properties and special physiological functions, which displays promising perspectives in the food and pharmaceutical industries. Currently, D-allulose is extremely sparse in nature and is predominantly biosynthesized through the isomerization ...
Caiming Li +2 more
exaly +3 more sources
Structural and Functional Features of Ketoso-3-Epimerases and Their Use in Production of D-Allulose
Биоорганическая химия, 2023Rare sugars attract more and more attention as safe, low-calorie sweeteners and functional compounds in the food, pharmaceutical and medical industries. The potential of the rare sugar D-allulose has been proven in a large number of theoretical and applied works but the high cost of its production is a limitation factor for its large-scall production ...
N. S. Ivanova +2 more
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Journal of Agricultural and Food Chemistryd-Allulose, a functional bulk sweetener, has recently attracted increasing attention because of its low-caloric-ness properties and diverse health effects.
Wenli Zhang, Hu Ren, Dawei Ni
exaly +2 more sources
Journal of Bioscience and Bioengineering, 2017
An enzyme that catalyzes C-3 epimerization between d-fructose and d-allulose was found in Arthrobacter globiformis strain M30. Arthrobacter species have long been used in the food industry and are well-known for their high degree of safety. The enzyme was purified by ion exchange and hydrophobic interaction chromatographies and characterized as a d ...
Tomoya Shintani +2 more
exaly +3 more sources
An enzyme that catalyzes C-3 epimerization between d-fructose and d-allulose was found in Arthrobacter globiformis strain M30. Arthrobacter species have long been used in the food industry and are well-known for their high degree of safety. The enzyme was purified by ion exchange and hydrophobic interaction chromatographies and characterized as a d ...
Tomoya Shintani +2 more
exaly +3 more sources
Enzyme and Microbial Technology, 2021
The rare sugar d-allulose is an attractive sucrose substitute due to its sweetness and ultra-low caloric value. It can be produced from D-fructose using d-allulose 3-epimerase (DAE) as the biocatalyst. However, most of the reported DAEs show low catalytic efficiency and poor thermostability, which limited their further use in food industrial.
Zhangliang Zhu +2 more
exaly +3 more sources
The rare sugar d-allulose is an attractive sucrose substitute due to its sweetness and ultra-low caloric value. It can be produced from D-fructose using d-allulose 3-epimerase (DAE) as the biocatalyst. However, most of the reported DAEs show low catalytic efficiency and poor thermostability, which limited their further use in food industrial.
Zhangliang Zhu +2 more
exaly +3 more sources
International Journal of Biological Macromolecules, 2021
The combined catalysis of glucose isomerase (GI) and D-psicose 3-epimerase (DPEase) provided a convenient route for the direct synthesis of D-allulose from d-glucose, whose cost is lower than d-fructose. In the present research, the weak activity of DPEase was the key rate-limiting step and resulted in the accumulation of d-fructose in engineered ...
Jingyi, Zhao +5 more
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The combined catalysis of glucose isomerase (GI) and D-psicose 3-epimerase (DPEase) provided a convenient route for the direct synthesis of D-allulose from d-glucose, whose cost is lower than d-fructose. In the present research, the weak activity of DPEase was the key rate-limiting step and resulted in the accumulation of d-fructose in engineered ...
Jingyi, Zhao +5 more
openaire +2 more sources
International Journal of Biological Macromolecules, 2021
Currently, low sugar and low energy have become an important trend in the food industries. Therefore, the bioconversion of the functional low-calorie rare sugars attracts more and more attention. l-Ribulose 3-epimerase (LREase) belongs to the ketose 3-epimerase (KEase) family, which could not only efficiently catalyze the reversible C-3 epimerization ...
Cuie Guang, Wenli Zhang, Wanmeng Mu
exaly +3 more sources
Currently, low sugar and low energy have become an important trend in the food industries. Therefore, the bioconversion of the functional low-calorie rare sugars attracts more and more attention. l-Ribulose 3-epimerase (LREase) belongs to the ketose 3-epimerase (KEase) family, which could not only efficiently catalyze the reversible C-3 epimerization ...
Cuie Guang, Wenli Zhang, Wanmeng Mu
exaly +3 more sources
Recent Advances in Ketose 3-Epimerase and Its Application for D-Allulose Production
2021In the past few decades, the number of people dealing with obesity, diabetes, hyperlipidemia, and hypertension has grown dramatically throughout the world. The main reason leading to this case is the high-sugar and high-fat diets (Van Laar et al. 2020). With the continuous development of human’s living standards, the health awareness of the publics has
Wenli Zhang, Jiajun Chen, Wanmeng Mu
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Enzyme and Microbial Technology, 2022
D-Allulose 3-epimerase (DAE) is promising to be used for the production of the rare sugar D-allulose in industry. However, the poor thermostability and low catalytic efficiency limited its large-scale industrial applications. A dual-enzyme screening method was developed to measure the activity of the D-allulose 3-epimerase from Clostridium ...
Zhongji Pu, Mianbin Wu, Lirong Yang
exaly +3 more sources
D-Allulose 3-epimerase (DAE) is promising to be used for the production of the rare sugar D-allulose in industry. However, the poor thermostability and low catalytic efficiency limited its large-scale industrial applications. A dual-enzyme screening method was developed to measure the activity of the D-allulose 3-epimerase from Clostridium ...
Zhongji Pu, Mianbin Wu, Lirong Yang
exaly +3 more sources