, 2019 Additional file 1: Table S1. Primers used for the construction of recombinant SaDAE. Figure S1. Multiple sequence alignment of amino acid sequence for the SaDAE with D-allulose 3-epimerase, L-ribulose 3-epimerase, and D-tagatose 3-epimerase from various strains. Figure S2. Purification of SaDAE by anion-exchange (a) and size-exclusion chromatography (b)
Zhu, Zhangliang +8 more
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Colorimetric method for rare sugars to distinguish aldoses and ketoses and its application [PDF]
, 2019 ケトース発色試薬の1つであるSi(IV)-Mo(VI)溶液による希少糖の発色特性(SiMo法)について調べた。本試薬はD-プシコース以外のケトースに対して速やかに発色を示した。これまで問題となっていたD-フルクトースとD-プシコースの比色法による区別を可能にする方法であることがわかった。すなわち，D-プシコースを基質としてケトース3-エピメラーゼ反応により生じたD-フルクトースをSiMo法によって他検体を同時にかつ容易に分析することが可能となった ...
Morimoto, Kenji +5 more
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食後脂質代謝に対する希少糖プシコースの効果に関する研究 [PDF]
, 2018 研究成果の概要（和文）：本研究は、果糖や果糖ブドウ糖液糖（HFS）による食後高脂血を防止する方策を明らかにすることを目的とした。果糖、HFSによる食後高脂血の増悪・遅延を、これらの甘味料を希少糖アルロースで置き換えて検討した。糖質代謝については脂肪と同時にアルロースを摂取した場合には、果糖やHFSと比べて優れていたが、脂質代謝については、これらと同様に食後代謝の遅延・増悪をきたした。市販の希少糖含有シロップ（RSS）では、HFSと比べて食後糖・脂質代謝に差は見られなかった。さらに ...
内藤 通孝
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Growing South Dakota (Spring/Summer 2020) [PDF]
, 2020 This issue contains the South Dakota Agricultural Experiment Station 2019 Annual Report. [Page] 2 South Dakota Agricultural Experiment Station Update[Page] 4 Featured Research[Page] 14 Graduate and Undergraduate Research: In Our Own Words[Page] 20 ...
Environmental Sciences, College of Agriculture, Food and
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Overexpression of luxS Promotes Stress Resistance and Biofilm Formation of Lactobacillus paraplantarum L-ZS9 by Regulating the Expression of Multiple Genes [PDF]
, 2018 Probiotics have evoked great interest in the past years for their beneficial effects. The aim of this study was to investigate whether luxS overexpression promotes the stress resistance of Lactobacillus paraplantarum L-ZS9.
Jinlan Zhang +7 more
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Research on heterologous expression of D-psicose-3-epimerase in Pichia pastoris [PDF]
D-allulose, a low-calorie and high-sweetness functional rare sugar, is regarded as an ideal alternative to traditional sweeteners in the health food industry due to its physiological benefits, such as regulating blood glucose metabolism and inhibiting ...
Fan, Qisheng
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Assessment of the potential risks in SD rats gavaged with genetically modified yeast containing the cp4-epsps gene [PDF]
IntroductionDespite the absence of definitive evidence indicating that the cp4-epsps gene and its resultant recombinant proteins have significant harmful effects on either human or animal health, the safety assessment of genetically modified (GM) crops ...
Bo Bi +7 more
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A new chemo-enzymatic approach to synthesize rare sugars using an engineered glycoside-3-oxidase [PDF]
Funding Information: We thank Diana Santos for the preliminary data and Teresa Silva and Cristina Tim\u00F3teo (Research Facilities, ITQB-NOVA) for their technical assistance.
Martins, Lígia O. +3 more
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Preparation and Properties Analysis of D-Allulose-3-Epimerase Cross-linked Enzyme Aggregates
Shipin gongye ke-jiThis study aimed to immobilize D-allulose 3-epimerase from Ruminococcus sp. (RDAE) using cross-linked enzyme aggregates (CLEAs) technology. The immobilized enzyme (RDAE-CLEA) was prepared by optimizing the precipitant polyethylene glycol (PEG4000 ...
Ronghui QIU +5 more
doaj +1 more source
Study on the fusion expression of L-rhamnose-isomerase and D-psicose-3-epimerase [PDF]
D-Allose, a rare cis-aldohexose with extensive physiological functions, has significant applications in food, healthcare, and pharmaceutical fields.
Li, Xiao
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