Results 51 to 60 of about 1,864,588 (148)

An Engineered Multi-Enzyme Cascade with Low-Cost ATP Regeneration for Efficient D-Allulose Production from D-Fructose

Fermentation
D-Allulose is a promising low-calorie rare sugar with significant health benefits. However, its industrial production is hindered by the low catalytic efficiency (≤33% conversion) and unfavorable equilibrium of the key enzyme, D-allulose 3-epimerase (DAE)
Yutong Lu, Huayang Tang, Dexun Fan, Qingzhu Wang, Shuangyan Han   +4 more
doaj   +1 more source

Research Progress Based on SpyTag/SpyCatcher Self‐Assembling Peptides and Their Applications in the Food Field

Food Bioengineering, Volume 4, Issue 3, Page 279-291, September 2025.
The formation of isopeptide bonds in the SpyTag/SpyCatcher system and its application in the food field ABSTRACT The SpyTag/SpyCatcher system is a modular protein assembly tool based on isopeptide bond‐mediated covalent conjugation, distinguished by mild reaction conditions, rapid ligation rate, and independence from exogenous enzymes or chemical ...
Meng‐meng Cai, Zi‐fu Ni, Zhong‐ke Sun, Xiao‐long Li, Zi‐hao Wang, Min Zhang, Cheng‐wei Li   +6 more
wiley   +1 more source

The crystal structure of the Y140F mutant of ADP-L-glycero-D-manno-heptose 6-epimerase bound to ADP-beta-D-mannose suggests a one base mechanism

, 2010
Supported by Wellcome Trust grant 081862/Z/06/ZBacteria synthesize a wide array of unusual carbohydrate molecules, which they use in a variety of ways.
Naismith, Jim, Kowatz, Thomas, Tanner, Martin E., Morrison, James P.   +3 more
core   +1 more source

Update: Enzymatische Synthese für industrielle Anwendungen

Angewandte Chemie, Volume 137, Issue 27, July 1, 2025.
Die Biokatalyse hat sich zu einer nachhaltigen und wettbewerbsfähigen Alternative zur herkömmlichen chemischen Synthese entwickelt und ermöglicht die enzymbasierte Herstellung nicht nur von chemischen Grundstoffen, sondern auch von (nicht natürlichen) Aminosäuren, (seltenen) Zuckern und synthetischen Nukleotiden.
Thomas Bayer, Shuke Wu, Radka Snajdrova, Kai Baldenius, Uwe T. Bornscheuer   +4 more
wiley   +1 more source

Improvement of D-Allulose Biocatalysis from D-Glucose in Engineered Escherichia coli by Enhancing Glucose Isomerase Expression and Substrate Supply

Fermentation
D-allulose is a rare low-calorie sugar with considerable health benefits and industrial potential. Compared with chemical synthesis and free enzyme catalysis, microbial production using engineered cells offers a low-cost and highly stable solution ...
Sheng Gao, Yinuo Li, Quan Cui, Chuanzhuang Guo, Jianbin Wang, Junlin Li, Ting Wang, Piwu Li, Jing Su, Ruiming Wang, Nan Li, Junqing Wang, Han Fan   +12 more
doaj   +1 more source

One pot cascade biosynthesis of D-allulose from D-glucose and its kinetic modelling

, 2022
One pot cascade of enzymatic catalysis was applied for u-allulose synthesis from ID-glucose by the combination of ID-glucose isomerase (GI) from Escherichia coli MG1655 and u-allulose 3-epimerase (DAE) from Agrobacterium tumefaciens C58.
Chen, Xiaoyan, Zhang, Yu, Wang, Zhongming, Wang, Wen, Yu, Qiang, Wang, Fan, Liu, Shijie, Yuan, Zhenhong   +7 more
core   +1 more source

Learning from Protein Engineering by Deconvolution of Multi‐Mutational Variants

Angewandte Chemie International Edition, Volume 63, Issue 36, September 2, 2024.
First derivative of a typical experimental enzyme fitness pathway landscape mapped on the basis of ΔG values, in this case of 24 trajectories in the deconvolution of a 4‐mutational variant. Green areas indicate negative slopes and define trajectories involved in energetically favored stages, while red areas indicate positive slopes representing ...
Frank Hollmann, Joaquin Sanchis, Manfred T. Reetz   +2 more
wiley   +1 more source

Study on Immobilization of Hyperthermophilic D-Allulose 3-Epimerase and Properties of Immobilized Enzyme(超嗜热D-阿洛酮糖3-差向异构酶的固定化及固定化酶性能研究) [PDF]

Shipin kexue jishu xuebao
D-allulose, a promising low-calorie natural sweetener and a key member of the rare sugar family, exhibits physiological benefits in mitigating multiple diseases.
SHEN Jidong(沈骥冬), XU Baoping(徐宝萍), HUANG Lianggang(黄良刚), LIU Zhiqiang(柳志强), ZHENG Yuguo(郑裕国)   +4 more
doaj   +1 more source

Thermostability Improvement of the d‑Allulose 3‑Epimerase from Dorea sp. CAG317 by Site-Directed Mutagenesis at the Interface Regions

, 2018
d-Allulose is a low-calorie sweetener and has broad applications in the food, cosmetics, and pharmaceutical industries. Recently, most studies focus on d-allulose production from d-fructose by d-allulose 3-epimerase (DAEase).
Ziwei Chen (626412), Cuie Guang (5307335), Tao Zhang (43681), Yanmin Zhang (391964), Jiawei Huang (4253152), Wanmeng Mu (408651), Wenli Zhang (146408)   +6 more
core   +1 more source

Enhanced Thermostability of D-Psicose 3-Epimerase from Clostridium bolteae through Rational Design and Engineering of New Disulfide Bridges

, 2021
D-psicose 3-epimerase (DPEase) catalyzes the isomerization of D-fructose to D-psicose (aka D-allulose, a low-calorie sweetener), but its industrial application has been restricted by the poor thermostability of the naturally available enzymes ...
Jing Chen, Huiyi Wang, Kai Li, Yan Guo, Jingyi Zhao, Jidong Liu   +5 more
core   +1 more source