Results 51 to 60 of about 270 (119)

Preparation and Properties Analysis of D-Allulose-3-Epimerase Cross-linked Enzyme Aggregates

Shipin gongye ke-ji
This study aimed to immobilize D-allulose 3-epimerase from Ruminococcus sp. (RDAE) using cross-linked enzyme aggregates (CLEAs) technology. The immobilized enzyme (RDAE-CLEA) was prepared by optimizing the precipitant polyethylene glycol (PEG4000 ...
Ronghui QIU, Ziyi LI, Jiao LI, Depei WANG, Yan ZENG, Yuanxia SUN   +5 more
doaj   +1 more source

One-site polarity switch enhances catalytic efficiency and stability of d-allulose 3-epimerase via flexibility–rigidity rebalancing

Scientific Reports
The structural foundation of enzyme efficiency frequently resides in the subtle equilibrium between localized flexibility and overarching rigidity.
Fina Amreta Laksmi, Yudhi Nugraha, Kenny Lischer, Khaerunnisa, David Herawan, Ario Betha Juanssilfero, Des Saputro Wibowo, Isa Nuryana, Syahputra Wibowo, Keni Vidilaseris, Mulyorini Rahayuningsih   +10 more
doaj   +1 more source

Construction of the Thermostable D-Allulose 3-Epimerase from Arthrobacter globiformis M30 by Protein Engineering Method.

Journal of applied glycoscience
D-Allulose 3-epimerase catalyzes C-3 epimerization between D-fructose and D-allulose was found in Arthrobacter globiformis strain M30. The enzyme gene was cloned, and its recombinant enzyme and the mutant variants were expressed in E. coli. Using the information of the sequence and model structure, we succeed in the improvement of melting temperature ...
Ohtani, Kouhei, Shimada, Kensaku, Gullapalli, Pushpa Kiran, Ishikawa, Kazuhiko   +3 more
openaire   +2 more sources

Literatur Review: D-Allulose 3-Epimerase from Microbial Sources and its Potential Uses

Jurnal Biologi Tropis
D-allulose, or psicose, is a rare, low-calorie sugar with various health benefits, such as managing blood sugar levels and lowering the risk of obesity, making it an ideal alternative to sucrose. In the food and pharmaceutical sectors, this study aims to review the characteristics and potential of D-allulose 3-epimerase (DAEase) enzymes from different ...
Fauziah Az-Zahra, Dwi Hilda Putri, Irdawati Irdawati, Dezi Handayani   +3 more
openaire   +1 more source

The Engineering, Expression, and Immobilization of Epimerases for D-allulose Production. [PDF]

Int J Mol Sci, 2023
Tan JH, Chen A, Bi J, Lim YH, Wong FT, Ow DS.   +5 more
europepmc   +1 more source

Establishment of the Biotransformation of D-Allulose and D-Allose Systems in Full-Red Jujube Monosaccharides. [PDF]

Plants (Basel), 2023
Liu F, Chen S, Pan F, Zhao Z, Liu M, Wang L.   +5 more
europepmc   +1 more source

Produce D-allulose from non-food biomass by integrating corn stalk hydrolysis with whole-cell catalysis. [PDF]

Front Bioeng Biotechnol, 2023
Jia Q, Zhang H, Zhao A, Qu L, Xiong W, Alam MA, Miao J, Wang W, Li F, Xu J, Lv Y.   +10 more
europepmc   +1 more source

Construction of the Thermostable D-Allulose 3-Epimerase from Arthrobacter globiformis M30 by Protein Engineering Method. [PDF]

J Appl Glycosci (1999)
Ohtani K, Shimada K, Gullapalli PK, Ishikawa K.   +3 more
europepmc   +1 more source

The Formation of D-Allulose 3-Epimerase Hybrid Nanoflowers and Co-Immobilization on Resins for Improved Enzyme Activity, Stability, and Processability. [PDF]

Int J Mol Sci
Ding W, Liu C, Huang C, Zhang X, Chi X, Wang T, Guo Q, Wang C.   +7 more
europepmc   +1 more source

Enhancing the thermostability of d-allulose 3-epimerase from Clostridium cellulolyticum H10 via directed evolution

Systems Microbiology and Biomanufacturing, 2022
Shuhan Liu, Yifan Wang, Demin Kong, Jing Wu, Zhanzhi Liu   +4 more
openaire   +1 more source