Ancestral Sequence Reconstruction Enhances Thermal Stability of D-Allulose 3-Epimerase
Shipin gongye ke-jiTo solve the problem of poor thermal stability of the current D-allulose 3-epimerase (DAEase), the ancestor sequences of DAEase with different catalytic domains were reconstructed by big data mining, reasonable modification and ancestor sequence ...
Lijun GUAN +9 more
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Thermostable D-Allulose 3-Epimerase for Long-Term Food-Compatible Continuous Production Systems
Applied SciencesD-allulose is a rare sugar with promising applications in food and health industries, owing to its low caloric value and multiple health benefits.
Jiawei Cui, Yan Li, Ming Yan
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A Novel d -Allulose 3-Epimerase Gene from the Metagenome of a Thermal Aquatic Habitat and d -Allulose Production by Bacillus subtilis Whole-Cell Catalysis [PDF]
Applied and Environmental Microbiology, 2020 Sudhir P Singh, Satya Narayan Patel
exaly +2 more sources
Production, purification, characterization, and safety evaluation of constructed recombinant D-psicose 3-epimerase [PDF]
Microbial Cell FactoriesBackground D-psicose 3-epimerase (DPEase) is a potential catalytic enzyme for D-psicose production. D-psicose, also known as D-allulose, is a low-calorie sweetener that has gained considerable attention as a healthy alternative sweetener due to its ...
Nisit Watthanasakphuban +5 more
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Immobilization of d-allulose 3-epimerase into magnetic metal–organic framework nanoparticles for efficient biocatalysis [PDF]
World Journal of Microbiology and Biotechnology, 2021 Abstract D-allulose is a rare low-calorie sugar that has many fundamental biological functions. D-allulose 3-epimerase from Agrobacterium tumefaciens (AT-DAEase) catalyzes the conversion of D-fructose to D-allulose. The enzyme has attracted considerable attention because of its mild catalytic properties.
Kai Xue +5 more
openaire +2 more sources
Frontiers in Bioengineering and Biotechnology, 2020 Rare sugar D-allulose as a substitute sweetener is produced through the isomerization of D-fructose by D-tagatose 3-epimerases (DTEases) or D-allulose 3-epimerases (DAEases).
Suwei Jiang +9 more
doaj +1 more source
Reaction Engineering and Comparison of Electroenzymatic and Enzymatic ATP Regeneration Systems
ChemElectroChem, Volume 10, Issue 22, November 14, 2023., 2023 An electrochemically coupled ATP regeneration by pyruvate oxidase and acetate kinase for the phosphorylation of mevalonate was established and expanded by a polyphosphate kinase. The reaction was characterized and compared with other ATP regenerating systems in terms of the phosphate donor properties and biocatalytic metrics.
Regine Siedentop +4 more
wiley +1 more source
Food manufacturing processes and technical data used in the exposure assessment of food enzymes
EFSA Journal, Volume 21, Issue 7, July 2023., 2023 Abstract Food enzymes are used for technical purposes in the production of food ingredients or foods‐as‐consumed. In the European Union, the safety of a food enzyme is evaluated by EFSA on the basis of a technical dossier provided by an applicant. Dietary exposure is an integral part of the risk assessment of food enzymes.
EFSA Panel on Food Contact Materials +25 more
wiley +1 more source
Foods, 2021 D-allulose is an epimer of D-fructose at the C-3 position. With similar sweetness to sucrose and a low-calorie profile, D-allulose has been considered a promising functional sweetener.
Yifan Bu +3 more
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Research Progress on Efficient Biocatalytic Synthesis of D-Allulose(D-阿洛酮糖的高效生物催化合成研究进展) [PDF]
Shipin kexue jishu xuebaoD-allulose is a novel low-calorie rare sugar with special physiological functions, including blood sugar regulation and antioxidant effects. In present, the biological synthesis of D-allulose mainly utilized D-allulose 3-epimerase to catalyze the ...
QIN Huimin(秦慧民) +4 more
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