Results 41 to 50 of about 270 (119)

Update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA 12: suitability of taxonomic units notified to EFSA until March 2020

EFSA Journal, Volume 18, Issue 7, July 2020., 2020
Abstract The qualified presumption of safety (QPS) was developed to provide a generic safety evaluation for biological agents to support EFSA's Scientific Panels. It is based on an assessment of the taxonomic identity, the body of knowledge, safety concerns and antimicrobial resistance.
EFSA Panel on Biological Hazards (BIOHAZ), Kostas Koutsoumanis, Ana Allende, Avelino Alvarez‐Ordóñez, Declan Bolton, Sara Bover‐Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Luisa Peixe, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Pier Sandro Cocconcelli, Pablo Salvador Fernández Escámez, Miguel Prieto Maradona, Amparo Querol, Juan Evaristo Suarez, Ingvar Sundh, Just Vlak, Fulvio Barizzone, Michaela Hempen, Lieve Herman   +26 more
wiley   +1 more source

Enzyme Immobilization on Synthesized Nanoporous Silica Particles and their Application in a Bi‐enzymatic Reaction

ChemCatChem, Volume 12, Issue 8, Page 2245-2252, April 20, 2020., 2020
Different immobilization methods were evaluated for application of two biocatalysts in a cofactor dependent reaction. Promising immobilization and activity yields were obtained by covalent immobilization on synthesized hydrophilic silica particles. High reaction stabilities for the two immobilized enzymes resulted in a 10‐fold increase of the turnover ...
Claudia Engelmann, Narendhiran Ekambaram, Jens Johannsen, Oliver Fellechner, Thomas Waluga, Georg Fieg, Andreas Liese, Paul Bubenheim   +7 more
wiley   +1 more source

Study on Synthesis of D-Allulose from D-Glucose Catalyzed by Fusion Proteins(融合蛋白催化D-葡萄糖合成D-阿洛酮糖的研究) [PDF]

Shipin kexue jishu xuebao
D-allulose is a novel functional sugar substitute primarily produced from the high-cost substrate D-fructose, using D-allulose 3-epimerase to achieve the conversion.
ZHANG Jiahe(张家赫), FENG Jingjie(冯敬杰), LIU Weiwei(刘微微), CHANG Chuting(常楚婷), GUO Qingbin(郭庆彬), DING Wentao(丁文涛), WANG Changlu(王昌禄)   +6 more
doaj   +1 more source

An Engineered Multi-Enzyme Cascade with Low-Cost ATP Regeneration for Efficient D-Allulose Production from D-Fructose

Fermentation
D-Allulose is a promising low-calorie rare sugar with significant health benefits. However, its industrial production is hindered by the low catalytic efficiency (≤33% conversion) and unfavorable equilibrium of the key enzyme, D-allulose 3-epimerase (DAE)
Yutong Lu, Huayang Tang, Dexun Fan, Qingzhu Wang, Shuangyan Han   +4 more
doaj   +1 more source

Research Progress Based on SpyTag/SpyCatcher Self‐Assembling Peptides and Their Applications in the Food Field

Food Bioengineering, Volume 4, Issue 3, Page 279-291, September 2025.
The formation of isopeptide bonds in the SpyTag/SpyCatcher system and its application in the food field ABSTRACT The SpyTag/SpyCatcher system is a modular protein assembly tool based on isopeptide bond‐mediated covalent conjugation, distinguished by mild reaction conditions, rapid ligation rate, and independence from exogenous enzymes or chemical ...
Meng‐meng Cai, Zi‐fu Ni, Zhong‐ke Sun, Xiao‐long Li, Zi‐hao Wang, Min Zhang, Cheng‐wei Li   +6 more
wiley   +1 more source

Improvement of D-Allulose Biocatalysis from D-Glucose in Engineered Escherichia coli by Enhancing Glucose Isomerase Expression and Substrate Supply

Fermentation
D-allulose is a rare low-calorie sugar with considerable health benefits and industrial potential. Compared with chemical synthesis and free enzyme catalysis, microbial production using engineered cells offers a low-cost and highly stable solution ...
Sheng Gao, Yinuo Li, Quan Cui, Chuanzhuang Guo, Jianbin Wang, Junlin Li, Ting Wang, Piwu Li, Jing Su, Ruiming Wang, Nan Li, Junqing Wang, Han Fan   +12 more
doaj   +1 more source

Update: Enzymatische Synthese für industrielle Anwendungen

Angewandte Chemie, Volume 137, Issue 27, July 1, 2025.
Die Biokatalyse hat sich zu einer nachhaltigen und wettbewerbsfähigen Alternative zur herkömmlichen chemischen Synthese entwickelt und ermöglicht die enzymbasierte Herstellung nicht nur von chemischen Grundstoffen, sondern auch von (nicht natürlichen) Aminosäuren, (seltenen) Zuckern und synthetischen Nukleotiden.
Thomas Bayer, Shuke Wu, Radka Snajdrova, Kai Baldenius, Uwe T. Bornscheuer   +4 more
wiley   +1 more source

Study on Immobilization of Hyperthermophilic D-Allulose 3-Epimerase and Properties of Immobilized Enzyme(超嗜热D-阿洛酮糖3-差向异构酶的固定化及固定化酶性能研究) [PDF]

Shipin kexue jishu xuebao
D-allulose, a promising low-calorie natural sweetener and a key member of the rare sugar family, exhibits physiological benefits in mitigating multiple diseases.
SHEN Jidong(沈骥冬), XU Baoping(徐宝萍), HUANG Lianggang(黄良刚), LIU Zhiqiang(柳志强), ZHENG Yuguo(郑裕国)   +4 more
doaj   +1 more source

Learning from Protein Engineering by Deconvolution of Multi‐Mutational Variants

Angewandte Chemie International Edition, Volume 63, Issue 36, September 2, 2024.
First derivative of a typical experimental enzyme fitness pathway landscape mapped on the basis of ΔG values, in this case of 24 trajectories in the deconvolution of a 4‐mutational variant. Green areas indicate negative slopes and define trajectories involved in energetically favored stages, while red areas indicate positive slopes representing ...
Frank Hollmann, Joaquin Sanchis, Manfred T. Reetz   +2 more
wiley   +1 more source

MOESM1 of Redesign of a novel d-allulose 3-epimerase from Staphylococcus aureus for thermostability and efficient biocatalytic production of d-allulose

, 2019
Additional file 1: Table S1. Primers used for the construction of recombinant SaDAE. Figure S1. Multiple sequence alignment of amino acid sequence for the SaDAE with D-allulose 3-epimerase, L-ribulose 3-epimerase, and D-tagatose 3-epimerase from various strains. Figure S2. Purification of SaDAE by anion-exchange (a) and size-exclusion chromatography (b)
Zhu, Zhangliang, Gao, Dengke, Li, Chao, Chen, Ying, Zhu, Menglu, Liu, Xin, Tanokura, Masaru, Qin, Hui-Min, Lu, Fuping   +8 more
openaire   +1 more source