The food enzyme d‐psicose 3‐epimerase (EC 5.1.3.30) is produced with the genetically modified Escherichia coli strain K‐12 W3110 (pWKLP) by Matsutani Chemical Industry Co., Ltd.
EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP) +24 more
doaj +2 more sources
Recent Advances Regarding the Physiological Functions and Biosynthesis of D-Allulose [PDF]
D-Allulose, a generally regarded as safe (GRAS) sugar, is rare in nature. It is among the most promising sweeteners for future use due to its low caloric content, sucrose-like taste, and unique functions.
Zhou Chen, Xiao-Dong Gao, Zijie Li
doaj +2 more sources
Safety of D-allulose as a novel food pursuant to Regulation (EU) 2015/2283. [PDF]
Abstract Following a request from the European Commission, the EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA) was asked to deliver an opinion on D‐allulose as a novel food (NF) pursuant to Regulation (EU) 2015/2283. During the risk assessment, the Panel identified a number of data gaps, which needed to be addressed by the applicant ...
EFSA Panel on Nutrition +29 more
europepmc +2 more sources
Gut microbial utilization of the alternative sweetener, D-allulose, via AlsE [PDF]
D-allulose, a rare sugar with emerging potential as a low-calorie sweetener, has garnered attention as an alternative to other commercially available alternative sweeteners, such as sugar alcohols, which often cause severe gastrointestinal discomfort.
Glory Minabou Ndjite +5 more
doaj +2 more sources
An Update: Enzymatic Synthesis for Industrial Applications. [PDF]
Biocatalysis has become a sustainable and cost‐competitive alternative to established chemical synthesis, enabling the enzyme‐based production of not only commodity chemicals but (non‐natural) amino acids, (rare) sugars, as well as synthetic nucleotides.
Bayer T +4 more
europepmc +2 more sources
Comprehensive Analysis of Allulose Production: A Review and Update [PDF]
Advancements in D-allulose production have seen significant strides in recent years, focusing on enzymatic conversion methods. Key developments include traditional immobilization techniques, the discovery of novel enzymes, directed evolution studies, and
Lei Wang +3 more
doaj +2 more sources
Sustainable Natural Product Glycosylation: A Critical Evaluation of Biocatalytic and Chemical Approaches. [PDF]
Developing a process to produce glycosylated natural products involves many decisions, and given the current climate crisis, the environmental impact of the various processes must be a major consideration. This review provides an overview of the various processes and a framework for which metrics should be quantified to select processes that are both ...
Mejia-Otalvaro F +3 more
europepmc +2 more sources
Engineering of Escherichia coli for D-allose fermentative synthesis from D-glucose through izumoring cascade epimerization [PDF]
D-Allose is a potential alternative to sucrose in the food industries and a useful additive for the healthcare products in the future. At present, the methods for large-scale production of D-allose are still under investigation, most of which are based ...
Ling-Jie Zheng +8 more
doaj +2 more sources
Research Advances of d-allulose: An Overview of Physiological Functions, Enzymatic Biotransformation Technologies, and Production Processes [PDF]
d-allulose has a significant application value as a sugar substitute, not only as a food ingredient and dietary supplement, but also with various physiological functions, such as improving insulin resistance, anti-obesity, and regulating glucolipid ...
Yu Xia +7 more
doaj +2 more sources
The DAEase gene sequence derived from Clostridium bolteae ATCCBAA-613 was synthesized by codon optimization. Using pCold TF as the expression vector, the cold-shock promoter CspA induced the expression of the DAEase gene in Escherichia coli BL21(DE3) at ...
Qiufeng LI +5 more
doaj +2 more sources

