Results 11 to 20 of about 270 (119)

Efficient D-allulose synthesis under acidic conditions by auto-inducing expression of the tandem D-allulose 3-epimerase genes in Bacillus subtilis [PDF]

Microbial Cell Factories, 2022
Background D-allulose, a hexulose monosaccharide with low calorie content and high sweetness, is commonly used as a functional sugar in food and nutrition.
Mengkai Hu, Yuxia Wei, Rongzhen Zhang, Minglong Shao, Taowei Yang, Meijuan Xu, Xian Zhang, Zhiming Rao   +7 more
doaj   +4 more sources

Biochemical characterization, structure-guided mutagenesis, and application of a recombinant D-allulose 3-epimerase from Christensenellaceae bacterium for the biocatalytic production of D-allulose [PDF]

Frontiers in Bioengineering and Biotechnology
D-Allulose has become a promising alternative sweetener due to its unique properties of low caloric content, moderate sweetness, and physiological effects. D-Allulose 3-epimerase (DAEase) is a promising enzyme for D-Allulose production.
Lijun Guan, Lijun Guan, Ling Zhu, Ling Zhu, Kunlun Wang, Kunlun Wang, Yang Gao, Yang Gao, Jialei Li, Jialei Li, Song Yan, Song Yan, Xindi Zhang, Xindi Zhang, Nina Ji, Jing Fan, Jing Fan, Ye Zhou, Ye Zhou, Xinmiao Yao, Xinmiao Yao, Bo Li, Bo Li   +22 more
doaj   +4 more sources

D-Allulose Production from D-Fructose by Permeabilized Recombinant Cells of Corynebacterium glutamicum Cells Expressing D-Allulose 3-Epimerase Flavonifractor plautii. [PDF]

PLoS ONE, 2016
A d-allulose 3-epimerase from Flavonifractor plautii was cloned and expressed in Escherichia coli and Corynebacterium glutamicum. The maximum activity of the enzyme purified from recombinant E.
Chul-Soon Park, Taeyong Kim, Seung-Hye Hong, Kyung-Chul Shin, Kyoung-Rok Kim, Deok-Kun Oh   +5 more
doaj   +6 more sources

Production of recombinant D-allulose 3-epimerase utilizing an auto-induction approach in fermentor cultures suitable for industrial application. [PDF]

PLoS ONE
D-Allulose 3-epimerase (DAEase) is the key enzyme catalyzing D-fructose to catalyze into D-allulose, a rare sugar in foods, which has lately drawn increasing worldwide attention owing to its possible health advantages and application as a substitute ...
Kenny Lischer, Fina Amreta Laksmi, Yudhi Nugraha, Helbert, Fauziah Az-Zahra, David Herawan, Ario Betha Juanssilfero, Des Saputro Wibowo, Kharisma Panji Ramadhan, Isa Nuryana, Mohd Shukuri Mohamad Ali   +10 more
doaj   +5 more sources

Biocatalytic Synthesis of D-Allulose Using Novel D-Tagatose 3-Epimerase From Christensenella minuta [PDF]

Frontiers in Chemistry, 2020
D-allulose, which is one of the important rare sugars, has gained significant attention in the food and pharmaceutical industries as a potential alternative to sucrose and fructose.
Wenjing Sun, Junhua Yun, Xianghui Qi
exaly   +5 more sources

Optimization of Ultrahigh-Throughput Screening Assay for Protein Engineering of d-Allulose 3-Epimerase [PDF]

Biomolecules, 2022
d-Allulose is the corresponding epimer of d-fructose at the C-3 position, which exhibits a similar taste and sweetness to sucrose. As a low-calorie sweetener, d-allulose has broad application prospects in the fields of medicine, food, and so on ...
Zhanzhi Liu, Shuhan Liu, Jingyi Jia, Liuxin Wang, Feng Wang, Xiaoyue Pan, Jing Wu, Sheng Chen   +7 more
doaj   +2 more sources

Safety evaluation of the food enzyme d‐psicose 3‐epimerase from the genetically modified Corynebacterium glutamicum strain FIS002

EFSA Journal, 2021
This assessment addresses a food enzyme preparation consisting of the immobilised intact but non‐viable cells of the genetically modified Corynebacterium glutamicum strain FIS002 by CJ‐Tereos Sweeteners Europe SAS. The production strain produces the food
EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP), Claude Lambré, José Manuel Barat Baviera, Claudia Bolognesi, Pier Sandro Cocconcelli, Riccardo Crebelli, David Michael Gott, Konrad Grob, Evgenia Lampi, Marcel Mengelers, Alicja Mortensen, Gilles Rivière, Inger‐Lise Steffensen, Christina Tlustos, Henk Van Loveren, Laurence Vernis, Holger Zorn, Boet Glandorf, Lieve Herman, Yi Liu, Joaquim Maia, Elsa Nielsen, Andrew Chesson   +22 more
doaj   +2 more sources

Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol [PDF]

Foods, 2022
Currently, the demand for low-calorie sweeteners has grown dramatically because consumers are more mindful of their health than they used to be. Therefore, bioproduction of low-calorie sweeteners from low-cost raw materials becomes a hot spot.
Jin Li, Jiajun Chen, Wei Xu, Wenli Zhang, Yeming Chen, Wanmeng Mu   +5 more
doaj   +2 more sources

Toxicological Assessment of D-Allulose From a Novel One-Step Fermentation Process Using Genetically Modified <i>Escherichia coli</i>: A 90-Day Dietary Toxicity Study in Rats. [PDF]

J Toxicol
D‐Allulose is a rare monosaccharide structurally similar to D‐fructose, characterized by low caloric content and relatively high sweetness. This study aimed to conduct a 90‐day oral toxicity test to systemically evaluate the potential toxicological effects of D‐allulose produced via a novel one‐step fermentation process using genetically engineered ...
Li Z, Nie Y, Du H, Zhang W, Zheng S, Cong W, Feng Y, Li G, Jing H, Ning J, Gao S.   +10 more
europepmc   +2 more sources

Optimization of fermentation conditions for whole cell catalytic synthesis of D-allulose by engineering Escherichia coli [PDF]

Scientific Reports
D-allulose/D-psicose is a significant rare sugar with broad applications in the pharmaceutical, food, and other industries. In this study, we cloned the D-allulose 3-epimerase (DPEase) gene from Arthrobacter globiformis M30, using pET22b as the vector ...
Haoran Liu, Kang Xu, ShuQi Sun, Yinbiao Wan, Bojia Zhang, Yang Song, Chuanzhuang Guo, Songsen Sui, Ruiming Wang, Piwu Li, Junqing Wang, Zhenshang Xu, Ting Wang   +12 more
doaj   +2 more sources