Racemases and epimerases operating through a 1,1-proton transfer mechanism: reactivity, mechanism and inhibition [PDF]
Chemical Society Reviews, 2021 Racemases and epimerases using a deprotonation/reprotonation mechanism are important drug targets and have important biotechnological applications. This review focuses on the reactivity, mechanism, and inhibition of these versatile enzymes.
Matthew D Lloyd +2 more
exaly +6 more sources
Uncovering a superfamily of nickel-dependent hydroxyacid racemases and epimerases [PDF]
Scientific Reports, 2020 AbstractIsomerization reactions are fundamental in biology. Lactate racemase, which isomerizes L- and D-lactate, is composed of the LarA protein and a nickel-containing cofactor, the nickel-pincer nucleotide (NPN). In this study, we show that LarA is part of a superfamily containing many different enzymes.
Benoit Desguin +2 more
exaly +7 more sources
Molecular and Mechanistic Characterization of PddB, the First PLP-Independent 2,4-Diaminobutyric Acid Racemase Discovered in an Actinobacterial D-Amino Acid Homopolymer Biosynthesis [PDF]
Frontiers in Microbiology, 2021 We recently disclosed that the biosynthesis of antiviral γ-poly-D-2,4-diaminobutyric acid (poly-D-Dab) in Streptoalloteichus hindustanus involves an unprecedented cofactor independent stereoinversion of Dab catalyzed by PddB, which shows weak homology to
Kazuya Yamanaka +5 more
doaj +2 more sources
Overcoming barriers for investigating nickel-pincer nucleotide cofactor-related enzymes [PDF]
mBioThe nickel-pincer nucleotide (NPN) cofactor is a modified pyridinium mononucleotide that tri-coordinates nickel and is crucial for the activity of certain racemases and epimerases. LarB, LarC, and LarE are responsible for NPN synthesis, with the cofactor
Jorge L. Nevarez +5 more
doaj +2 more sources
Unveiling 14 novel 2-hydroxy acid racemization and epimerization reactions in the lactate racemase superfamily [PDF]
Journal of Biological ChemistryBenoit Desguin, Julian Urdiain-Arraiza
exaly +2 more sources
Prevalence of proline racemase/ hydroxyproline epimerase gene in human brucella isolates in Iran [PDF]
Medical Journal of the Islamic Republic of Iran, 2017 Background: Human brucellosis is a zoonotic disease caused by Brucella melitensis, Brucella abortus, and Brucella suis. Brucella causes a chronic disease, which subverts the immune defense system of their hosts. In this study, the prevalence of an important Brucella virulence determinant, prpA, which can modulate immune response, was determined in ...
Hashemifar, Iman +3 more
openaire +2 more sources
Identification, Purification, and Characterization of a Novel Amino Acid Racemase, Isoleucine 2-Epimerase, from Lactobacillus Species [PDF]
Journal of Bacteriology, 2013 ABSTRACT Accumulation of d -leucine, d - allo -isoleucine, and d -valine was observed in the growth medium of a lactic acid bacterium, Lactobacillus otakiensis JCM 15040, and the racemase responsible was ...
Yuta, Mutaguchi +4 more
openaire +2 more sources
Infection and Immunity, 2020 The Chlamydia trachomatis genome encodes multiple bifunctional enzymes, such as DapF, which is capable of both diaminopimelic acid (DAP) epimerase and glutamate racemase activity. Our previous work demonstrated the bifunctional activity of chlamydial DapF in vitro and in a heterologous system
Raghuveer Singh +5 more
openaire +3 more sources
PLoS ONE, 2007 The first eukaryotic proline racemase (PRAC), isolated from the human Trypanosoma cruzi pathogen, is a validated therapeutic target against Chagas' disease. This essential enzyme is implicated in parasite life cycle and infectivity and its ability to trigger host B-cell nonspecific hypergammaglobulinemia contributes to parasite evasion and persistence.
Goytia, Maira +6 more
openaire +4 more sources
PLOS ONE, 2015 Proline racemase (ProR) is a member of the pyridoxal 5'-phosphate-independent racemase family, and is involved in the Stickland reaction (fermentation) in certain clostridia as well as the mechanisms underlying the escape of parasites from host immunity in eukaryotic Trypanosoma.
Seiya Watanabe +3 more
openaire +4 more sources