Results 31 to 40 of about 632 (113)

Uncovering a superfamily of nickel-dependent hydroxyacid racemases and epimerases

, 2021
Isomerization reactions are fundamental in biology. Lactate racemase, which isomerizes L- and D-lactate, is composed of the LarA protein and a nickel-containing cofactor, the nickel-pincer nucleotide (NPN). In this study, we show that LarA is part of a superfamily containing many different enzymes.
Urdiain-Arraiza, Julian, World Microbe Forum   +1 more
openaire   +2 more sources

A food-grade expression system for d-psicose 3-epimerase production in Bacillus subtilis using an alanine racemase-encoding selection marker

Bioresources and Bioprocessing, 2017
Food-grade expression systems require that the resultant strains should only contain materials from food-safe microorganisms, and no antibiotic resistance marker can be utilized. To develop a food-grade expression system for d-psicose 3-epimerase production, we use an alanine racemase-encoding gene as selection marker in Bacillus subtilis.In this study,
Chen, Jingqi, Jin, Zhaoxia, Gai, Yuanming, Sun, Jibin, Zhang, Dawei   +4 more
openaire   +2 more sources

Structures of two LarA‐like nickel‐pincer nucleotide cofactor‐utilizing enzymes with a single catalytic histidine residue

Protein Science, Volume 34, Issue 12, December 2025.
Abstract The nickel‐pincer nucleotide (NPN) cofactor catalyzes the racemization/epimerization of α‐hydroxy acids in enzymes of the LarA family. The established proton‐coupled hydride transfer mechanism requires two catalytic histidine residues that alternately act as general acids and general bases. Notably, however, a fraction of LarA homologs (LarAHs)
Santhosh Gatreddi, Sundharraman Subramanian, Dexin Sui, Tianqi Wang, Julian Urdiain‐Arraiza, Benoît Desguin, Robert P. Hausinger, Kristin N. Parent, Jian Hu   +8 more
wiley   +1 more source

New Insights into the Reaction Mechanism Catalyzed by the Glutamate Racemase Enzyme:  pH Titration Curves and Classical Molecular Dynamics Simulations

, 2016
The mechanism of the reactions catalyzed by the pyridoxal-phosphate-independent amino acid racemases and epimerases faces the difficult task of deprotonating a relatively low acidicity proton, the amino acid's α-hydrogen, with a relatively poor base, a ...
Àngels González-Lafont (2458606), José M. Lluch (2458609), Eduard Puig (2386030), Martin J. Field (1317396), Mireia Garcia-Viloca (1554649)   +4 more
core   +1 more source

Intramolecular epistasis correlates with divergence of specificity in promiscuous and bifunctional NSAR/OSBS enzymes

Protein Science, Volume 34, Issue 5, May 2025.
Abstract Understanding the functions and evolution of specificity‐determining residues is essential for improving strategies to predict and design enzyme functions. Whether the function of an amino acid residue is retained during evolution depends on intramolecular epistasis, which occurs when the same residue contributes to different phenotypes in ...
Dat P. Truong, Roopa Dharmatti, Dylan Suriadinata, Jamison Huddleston, Rebecca Skouby, Gladys Owusu Addo, Mingzhao Zhu, Anjana Delpe Acharige, Reethu Sankari Bayana, Cristian Davila, Susan C. Fults, Frank M. Raushel, Kenneth G. Hull, Daniel Romo, Margaret E. Glasner   +14 more
wiley   +1 more source

Comparative Transcriptomic Analysis of AtBBX29 Transgenic and Wild Type Sugarcane Exposed to Drought Stress

Physiologia Plantarum, Volume 177, Issue 3, May/June 2025.
ABSTRACT Previously, we produced drought‐tolerant transgenic sugarcane plants that overexpressed the AtBBX29 gene, which encodes a transcription factor (TF) B‐box protein. These plants displayed delayed senescence, were able to maintain photosynthesis, and accumulated high levels of antioxidants and osmolytes when exposed to water deficit stress ...
Christell van der Vyver, Preetom Regon, Kristen Fulton, Sanjib K. Panda   +3 more
wiley   +1 more source

Liquid Chromatographic and Capillary Electromigration Techniques for the Analysis of Azole Antifungals

Journal of Separation Science, Volume 48, Issue 4, April 2025.
ABSTRACT Since the introduction of azoles as antifungals in 1979, they gradually gained a spotlight in both medicinal and industrial fields. Other than being the leading antifungal medicinal drug agents, azoles have also been a major pesticide in agricultural industry.
Tuuliina Tuominen, Dumidu Perera, Giacomo Russo, Susanne K. Wiedmer   +3 more
wiley   +1 more source

Proteomic analysis across aged tissues reveals distinct signatures and the crucial involvement of midgut barrier function in the regulation of aging

Aging Cell, Volume 24, Issue 1, January 2025.
Proteomic analysis reveals that instead of specific proteins, different tissues exhibit common dysregulated functional networks. Specifically, the integrity of the midgut barrier function is compromised during aging, which can be restored through the downregulation of the insulin receptor (InR).
Congying Zhang, Jinlong Wang, Tianzhao Yao, Jiaxin Hu, Feifei Sun, Chunlu Feng, Zhendong Sun, Yuzhuo Shao, Zhu Wang, Jiarui Wu, Yunpeng Huang   +10 more
wiley   +1 more source

Identification of the Cirratiomycin Biosynthesis Gene Cluster in Streptomyces Cirratus: Elucidation of the Biosynthetic Pathways for 2,3‐Diaminobutyric Acid and Hydroxymethylserine

Chemistry – A European Journal, Volume 30, Issue 28, May 17, 2024.
The cirratiomycin biosynthetic gene cluster was identified in Streptomyces cirratus by genome sequencing and gene inactivation. The biosynthesis of two nonproteinogenic amino acids was elucidated: (2S,3S)‐2,3‐diaminobutyric acid (DABA) is synthesized from threonine by four enzymes, CirQ, CirR, CirS and CirB, and α‐(hydroxymethyl)serine (HMSer) is ...
Shunki Sakata, Jiafeng Li, Yoko Yasuno, Tetsuro Shinada, Kazuo Shin‐ya, Yohei Katsuyama, Yasuo Ohnishi   +6 more
wiley   +1 more source

Experimental evidence of d‐glutamate racemase activity in the uncultivated bacterium Candidatus Saccharimonas aalborgensis

Environmental Microbiology, Volume 26, Issue 4, April 2024.
Bacteria from the uncultivated Candidate Phyla Radiation (CPR) dominate various habitats, despite possessing limited metabolic capacities. These bacteria have incomplete sets of enzymes, which include pathways for producing structures that are essential for life. This supports the hypothesis of moonlighting activities in the remaining enzymes.
Marcos Peñalver, Alberto Paradela, César Palacios‐Cuéllar, M. Graciela Pucciarelli, Francisco García‐del Portillo   +4 more
wiley   +1 more source